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Previous work has shown that Suc1/Cks proteins can promote the hyperphosphorylation of primed Cdk1 substrates through the formation of ternary Cdk1-Cks-phosphosubstrate complexes. This raises the possibility that Cks proteins might be able to both facilitate and interfere with hyperphosphorylation through a mechanism analogous to the prozone effect in antigen-antibody interactions, with substoichiometric Cks promoting the formation of Cdk1-Cks-phosphosubstrate complexes and suprastoichiometric Cks instead promoting the formation of Cdk1-Cks and Cks-phosphosubstrate complexes. We tested this hypothesis through a combination of theory, proof-of-principle experiments with oligonucleotide annealing, and experiments on the interaction of Xenopus cyclin B1-Cdk1-Cks2 with Wee1A in vitro and in Xenopus extracts. Our findings help explain why both Cks under-expression and overexpression interfere with cell-cycle progression and provide insight into the regulation of the Cdk1 system.
Arvai,
Crystallization and preliminary crystallographic study of human CksHs1: a cell cycle regulatory protein.
1995, Pubmed
Arvai,
Crystallization and preliminary crystallographic study of human CksHs1: a cell cycle regulatory protein.
1995,
Pubmed
Basi,
p13suc1 of Schizosaccharomyces pombe regulates two distinct forms of the mitotic cdc2 kinase.
1995,
Pubmed
Bourne,
Crystal structure and mutational analysis of the Saccharomyces cerevisiae cell cycle regulatory protein Cks1: implications for domain swapping, anion binding and protein interactions.
2000,
Pubmed
Bourne,
Crystal structure and mutational analysis of the human CDK2 kinase complex with cell cycle-regulatory protein CksHs1.
1996,
Pubmed
Bourne,
Crystal structure of the cell cycle-regulatory protein suc1 reveals a beta-hinge conformational switch.
1995,
Pubmed
Bray,
Computer-based analysis of the binding steps in protein complex formation.
1997,
Pubmed
Cacace,
Identification of constitutive and ras-inducible phosphorylation sites of KSR: implications for 14-3-3 binding, mitogen-activated protein kinase binding, and KSR overexpression.
1999,
Pubmed
,
Xenbase
Cahill,
Regulatory squelching.
1994,
Pubmed
Dickens,
A cytoplasmic inhibitor of the JNK signal transduction pathway.
1997,
Pubmed
Dunphy,
Fission yeast p13 blocks mitotic activation and tyrosine dephosphorylation of the Xenopus cdc2 protein kinase.
1989,
Pubmed
,
Xenbase
Endicott,
The crystal structure of p13suc1, a p34cdc2-interacting cell cycle control protein.
1995,
Pubmed
Ferrell,
Ultrasensitivity part II: multisite phosphorylation, stoichiometric inhibitors, and positive feedback.
2014,
Pubmed
Guertin,
Transient estrogen receptor binding and p300 redistribution support a squelching mechanism for estradiol-repressed genes.
2014,
Pubmed
Gunawardena,
Multisite protein phosphorylation makes a good threshold but can be a poor switch.
2005,
Pubmed
Hayles,
suc1 is an essential gene involved in both the cell cycle and growth in fission yeast.
1986,
Pubmed
Heidelberger,
A QUANTITATIVE STUDY OF THE PRECIPITIN REACTION BETWEEN TYPE III PNEUMOCOCCUS POLYSACCHARIDE AND PURIFIED HOMOLOGOUS ANTIBODY.
1929,
Pubmed
Huang,
Ultrasensitivity in the mitogen-activated protein kinase cascade.
1996,
Pubmed
,
Xenbase
Jullien,
LUEGO: a cost and time saving gel shift procedure.
2011,
Pubmed
Kim,
Substrate competition as a source of ultrasensitivity in the inactivation of Wee1.
2007,
Pubmed
,
Xenbase
Kim,
Multisite M-phase phosphorylation of Xenopus Wee1A.
2005,
Pubmed
,
Xenbase
Kõivomägi,
Multisite phosphorylation networks as signal processors for Cdk1.
2013,
Pubmed
Kumagai,
Control of the Cdc2/cyclin B complex in Xenopus egg extracts arrested at a G2/M checkpoint with DNA synthesis inhibitors.
1995,
Pubmed
,
Xenbase
Lee,
Positive regulation of Wee1 by Chk1 and 14-3-3 proteins.
2001,
Pubmed
,
Xenbase
Levchenko,
Scaffold proteins may biphasically affect the levels of mitogen-activated protein kinase signaling and reduce its threshold properties.
2000,
Pubmed
Markevich,
Signaling switches and bistability arising from multisite phosphorylation in protein kinase cascades.
2004,
Pubmed
Martinsson-Ahlzén,
Cyclin-dependent kinase-associated proteins Cks1 and Cks2 are essential during early embryogenesis and for cell cycle progression in somatic cells.
2008,
Pubmed
McGrath,
Cks confers specificity to phosphorylation-dependent CDK signaling pathways.
2013,
Pubmed
Moreno,
Regulation of p34cdc2 protein kinase during mitosis.
1989,
Pubmed
Mueller,
Cell cycle regulation of a Xenopus Wee1-like kinase.
1995,
Pubmed
,
Xenbase
Murray,
Cell cycle extracts.
1991,
Pubmed
Natesan,
Transcriptional squelching re-examined.
1997,
Pubmed
Owczarzy,
Stability and mismatch discrimination of locked nucleic acid-DNA duplexes.
2011,
Pubmed
Parge,
Human CksHs2 atomic structure: a role for its hexameric assembly in cell cycle control.
1993,
Pubmed
Patra,
Xe-p9, a Xenopus Suc1/Cks homolog, has multiple essential roles in cell cycle control.
1996,
Pubmed
,
Xenbase
Patra,
Xe-p9, a Xenopus Suc1/Cks protein, is essential for the Cdc2-dependent phosphorylation of the anaphase- promoting complex at mitosis.
1998,
Pubmed
,
Xenbase
Patra,
The xenopus Suc1/Cks protein promotes the phosphorylation of G(2)/M regulators.
1999,
Pubmed
,
Xenbase
Pettersen,
UCSF Chimera--a visualization system for exploratory research and analysis.
2004,
Pubmed
Pines,
Cell cycle: reaching for a role for the Cks proteins.
1996,
Pubmed
Pomerening,
Systems-level dissection of the cell-cycle oscillator: bypassing positive feedback produces damped oscillations.
2005,
Pubmed
,
Xenbase
Prywes,
In vitro squelching of activated transcription by serum response factor: evidence for a common coactivator used by multiple transcriptional activators.
1992,
Pubmed
Roy,
KSR is a scaffold required for activation of the ERK/MAPK module.
2002,
Pubmed
Sadaie,
Quantitative in vivo fluorescence cross-correlation analyses highlight the importance of competitive effects in the regulation of protein-protein interactions.
2014,
Pubmed
Trunnell,
Ultrasensitivity in the Regulation of Cdc25C by Cdk1.
2011,
Pubmed
,
Xenbase
Walter,
Activation of Wee1 by p42 MAPK in vitro and in cycling xenopus egg extracts.
2000,
Pubmed
,
Xenbase
Wang,
Nonessential sites improve phosphorylation switch.
2010,
Pubmed
,
Xenbase
Wühr,
Deep proteomics of the Xenopus laevis egg using an mRNA-derived reference database.
2014,
Pubmed
,
Xenbase
Yasuda,
The JIP group of mitogen-activated protein kinase scaffold proteins.
1999,
Pubmed
Yu,
Cks1 is dispensable for survival in Saccharomyces cerevisiae.
2004,
Pubmed
