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XB-ART-51987
Nanomedicine (Lond) 2016 Mar 01;116:643-56. doi: 10.2217/nnm.15.219.
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An early developmental vertebrate model for nanomaterial safety: bridging cell-based and mammalian toxicity assessment.

Webster CA , Di Silvio D , Devarajan A , Bigini P , Micotti E , Giudice C , Salmona M , Wheeler GN , Sherwood V , Bombelli FB .


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AIM: With the rise in production of nanoparticles (NPs) for an ever-increasing number of applications, there is an urgent need to efficiently assess their potential toxicity. We propose a NP hazard assessment protocol that combines mammalian cytotoxicity data with embryonic vertebrate abnormality scoring to determine an overall toxicity index. RESULTS: We observed that, after exposure to a range of NPs, Xenopus phenotypic scoring showed a strong correlation with cell based in vitro assays. Magnetite-cored NPs, negative for toxicity in vitro and Xenopus, were further confirmed as nontoxic in mice. CONCLUSION: The results highlight the potential of Xenopus embryo analysis as a fast screening approach for toxicity assessment of NPs, which could be introduced for the routine testing of nanomaterials.

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Species referenced: Xenopus laevis
Genes referenced: nps