Wlizla M et al. (2017), Luteinizing Hormone is an effective replacement...

XB-ART-52253

Dev Biol 2017 Jun 15;4262:442-448. doi: 10.1016/j.ydbio.2016.05.028.

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Luteinizing Hormone is an effective replacement for hCG to induce ovulation in Xenopus.

Wlizla M , Falco R , Peshkin L , Parlow AF , Horb ME .

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Injection of human Chorionic Gonadotropin (hCG) directly into the dorsal lymph sac of Xenopus is a commonly used protocol for induction of ovulation, but recent shortages in the stocks of commercially available hCG as well as lack of a well tested alternative have resulted in frustrating experimental delays in laboratories that predominantly use Xenopus in their research. Mammalian Luteinizing Hormones (LH) share structural similarity, functional equivalency, and bind the same receptor as hCG; this suggests that LH may serve as a good alternative to hCG for promoting ovulation in Xenopus. LH has been found to induce maturation of Xenopus oocytes in vitro, but whether it can be used to induce ovulation in vivo has not been examined. Here we compared the ability of four mammalian LH proteins, bovine (bLH), human (hLH), ovine (oLH), porcine (pLH), to induce ovulation in Xenopus when injected into the dorsal lymph sac of sexually mature females. We find that both ovine and human LH, but not bovine or porcine, are good substitutes for hCG for induction of ovulation in WT and J strain Xenopus laevis and Xenopus tropicalis.

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Species referenced: Xenopus tropicalis Xenopus laevis
Genes referenced: rhcg

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	Fig. 1. Ovine Luteinizing Hormone efficiently induces spawning in both J strain and WT X. laevis, independent of egg laying history. (A) J strain o+, (B) WT o+, (C) J strain virgin, and (D) WT virgin X. laevis females were boosted with either hCG or oLH. The height of each bar indicates the average number of eggs laid per female with the error bars showing the standard error of the mean. The ratios within each bar represent the corresponding number of females that laid eggs per number of females boosted. Only females that laid were included in the calculations of average number of eggs laid and the standard error of the mean. The amount and type of hormone used are indicated on the bottom of each graph.
	Fig. 2. Efficiency of spawning in J strain and WT o+ females boosted with either bLH, hLH, or pLH. (A) J strain o+, and (B) WT o+ X. laevis females were boosted with either bLH, hLH, or pLH. The height of each bar indicates the average number of eggs laid per female with the error bars showing the standard error of the mean. The ratios within or above each bar represent the corresponding number of females that laid eggs per number of females boosted. Only females that laid were included in the calculations of average number of eggs laid and the standard error of the mean. The amount and type of hormone used are indicated beneath the bars.
	Fig. 3. Spawning efficiency in X. tropicalis females first primed with either hCG or PMSG and then boosted with hCG, rhCG, or oLH. X. tropicalis females were primed with either 10 IU of hCG (green bars) or 15 IU of PMSG (blue bars). The height of each bar indicates the average number of eggs laid per female with the error bars showing the standard error of the mean. The ratios within each bar represent the number of females that laid eggs per number of females boosted. Only females that laid were included in the calculations of average number of eggs laid and the standard error of the mean. The amount and type of hormone used are indicated on the bottom of the graph.

References [+] :

BERGERS, Amphibian ovulation in vitro induced by mammalian pituitary hormones and progesterone. 1960, Pubmed