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Pflugers Arch
2016 Aug 01;4688:1363-74. doi: 10.1007/s00424-016-1842-5.
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Amino acid transporter B(0)AT1 (slc6a19) and ancillary protein: impact on function.
Margheritis E
,
Imperiali FG
,
Cinquetti R
,
Vollero A
,
Terova G
,
Rimoldi S
,
Girardello R
,
Bossi E
.
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Amino acids play an important role in the metabolism of all organisms. Their epithelial re-absorption is due to specific transport proteins, such as B(0)AT1, a Na(+)-coupled neutral amino acid symporter belonging to the solute carrier 6 family. Here, a recently cloned fish orthologue, from the intestine of Salmo salar, was electrophysiologically characterized with the two-electrode voltage clamp technique, in Xenopus laevis oocytes heterologously expressing the transporter. Substrate specificity, apparent affinities and the ionic dependence of the transport mechanism were determined in the presence of specific collectrin. Results demonstrated that like the human, but differently from sea bass (Dicentrarchus labrax) orthologue, salmon B(0)AT1 needs to be associated with partner proteins to be correctly expressed at the oocyteplasma membrane. Cloning of sea bass collectrin and comparison of membrane expression and functionality of the B(0)AT1 orthologue transporters allowed a deeper investigation on the role of their interactions. The parameters acquired by electrophysiological and immunolocalization experiments in the mammalian and fish transporters contributed to highlight the dynamic of relations and impacts on transport function of the ancillary proteins. The comparative characterization of the physiological parameters of amino acid transporters with auxiliary proteins can help the comprehension of the regulatory mechanism of essential nutrient absorption.
Böhmer,
Characterization of mouse amino acid transporter B0AT1 (slc6a19).
2005,
Pubmed
,
Xenbase
Bossi,
Ionic selectivity of the coupled and uncoupled currents carried by the amino acid transporter KAAT1.
1999,
Pubmed
,
Xenbase
Bossi,
Temperature effects on the kinetic properties of the rabbit intestinal oligopeptide cotransporter PepT1.
2012,
Pubmed
,
Xenbase
Bossi,
Residues R282 and D341 act as electrostatic gates in the proton-dependent oligopeptide transporter PepT1.
2011,
Pubmed
,
Xenbase
Bossi,
Ion binding and permeation through the lepidopteran amino acid transporter KAAT1 expressed in Xenopus oocytes.
1999,
Pubmed
,
Xenbase
Bröer,
Molecular cloning of mouse amino acid transport system B0, a neutral amino acid transporter related to Hartnup disorder.
2004,
Pubmed
Bröer,
The molecular basis of neutral aminoacidurias.
2006,
Pubmed
Camargo,
Steady-state kinetic characterization of the mouse B(0)AT1 sodium-dependent neutral amino acid transporter.
2005,
Pubmed
,
Xenbase
Camargo,
Tissue-specific amino acid transporter partners ACE2 and collectrin differentially interact with hartnup mutations.
2009,
Pubmed
,
Xenbase
Cheon,
Novel mutation in SLC6A19 causing late-onset seizures in Hartnup disorder.
2010,
Pubmed
Daniel,
The proton oligopeptide cotransporter family SLC15 in physiology and pharmacology.
2004,
Pubmed
Danilczyk,
Essential role for collectrin in renal amino acid transport.
2006,
Pubmed
,
Xenbase
Fairweather,
Molecular basis for the interaction of the mammalian amino acid transporters B0AT1 and B0AT3 with their ancillary protein collectrin.
2015,
Pubmed
,
Xenbase
Forrest,
The rocking bundle: a mechanism for ion-coupled solute flux by symmetrical transporters.
2009,
Pubmed
Hashimoto,
ACE2 links amino acid malnutrition to microbial ecology and intestinal inflammation.
2012,
Pubmed
Imai,
Angiotensin-converting enzyme 2 (ACE2) in disease pathogenesis.
2010,
Pubmed
Kleta,
Mutations in SLC6A19, encoding B0AT1, cause Hartnup disorder.
2004,
Pubmed
Kowalczuk,
A protein complex in the brush-border membrane explains a Hartnup disorder allele.
2008,
Pubmed
,
Xenbase
Margheritis,
Functional properties of a newly cloned fish ortholog of the neutral amino acid transporter B0AT1 (SLC6A19).
2013,
Pubmed
,
Xenbase
McCoy,
Collectrin/tmem27 is expressed at high levels in all segments of the developing Xenopus pronephric nephron and in the Wolffian duct.
2008,
Pubmed
,
Xenbase
Mertl,
Substrate-induced changes in the density of peptide transporter PEPT1 expressed in Xenopus oocytes.
2008,
Pubmed
,
Xenbase
Nelson,
The significance of molecular slips in transport systems.
2002,
Pubmed
Peres,
Electrophysiological insights into the mechanism of ion-coupled cotransporters.
2004,
Pubmed
Renna,
Functional and structural determinants of reverse operation in the pH-dependent oligopeptide transporter PepT1.
2011,
Pubmed
,
Xenbase
Renna,
Unified modeling of the mammalian and fish proton-dependent oligopeptide transporter PepT1.
2011,
Pubmed
,
Xenbase
Rimoldi,
Intestinal B(0)AT1 (SLC6A19) and PEPT1 (SLC15A1) mRNA levels in European sea bass (Dicentrarchus labrax) reared in fresh water and fed fish and plant protein sources.
2015,
Pubmed
Romano,
Teleost fish models in membrane transport research: the PEPT1(SLC15A1) H+-oligopeptide transporter as a case study.
2014,
Pubmed
Rudnick,
The SLC6 transporters: perspectives on structure, functions, regulation, and models for transporter dysfunction.
2014,
Pubmed
Sangaletti,
Functional expression of the oligopeptide transporter PepT1 from the sea bass (Dicentrarchus labrax).
2009,
Pubmed
,
Xenbase
Seow,
Hartnup disorder is caused by mutations in the gene encoding the neutral amino acid transporter SLC6A19.
2004,
Pubmed
Singer,
Orphan transporter SLC6A18 is renal neutral amino acid transporter B0AT3.
2009,
Pubmed
,
Xenbase
Singer,
Collectrin and ACE2 in renal and intestinal amino acid transport.
2011,
Pubmed
Vollero,
The D-amino acid transport by the invertebrate SLC6 transporters KAAT1 and CAATCH1 from Manduca sexta.
2016,
Pubmed
,
Xenbase
Yamashita,
Crystal structure of a bacterial homologue of Na+/Cl--dependent neurotransmitter transporters.
2005,
Pubmed
