Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Am J Physiol Cell Physiol
2016 Dec 01;3116:C945-C959. doi: 10.1152/ajpcell.00259.2016.
Show Gene links
Show Anatomy links
Mouse Slc4a11 expressed in Xenopus oocytes is an ideally selective H+/OH- conductance pathway that is stimulated by rises in intracellular and extracellular pH.
???displayArticle.abstract???
The SLC4A11 gene encodes the bicarbonate-transporter-related protein BTR1, which is mutated in syndromes characterized by vision and hearing loss. Signs of these diseases [congenital hereditary endothelial dystrophy (CHED) and Harboyan syndrome] are evident in mouse models of Slc4a11 disruption. However, the intrinsic activity of Slc4a11 remains controversial, complicating assignment of its (patho)physiological role. Most studies concur that Slc4a11 transports H+ (or the thermodynamically equivalent species OH-) rather than HCO3-, but disparities have arisen as to whether the transport is coupled to another species such as Na+ or NH3/NH4+ Here for the first time, we examine the action of mouse Slc4a11 in Xenopus oocytes. We simultaneously monitor changes in intracellular pH, membrane potential, and conductance as we alter extracellular pH, revealing the electrical and chemical driving forces that underlie the observed ion fluxes. We find that mSlc4a11 is an ideally selective H+/OH- conductive pathway, the action of which is uncoupled from the cotransport of any other ion. We also find that the activity of mSlc4a11 is independently enhanced by both extracellular and intracellular alkalinization, suggesting OH- as the most likely substrate and providing a novel explanation for the apparent NH3-dependence of Slc4a11-mediated currents reported by others. We suggest that the unique properties of Slc4a11 action underlie its value as a pH regulator in corneal endothelial cells.
Allert,
Ammonia-induced depolarization of cultured rat cortical astrocytes.
1998, Pubmed
Allert,
Ammonia-induced depolarization of cultured rat cortical astrocytes.
1998,
Pubmed
Becker,
Facilitated lactate transport by MCT1 when coexpressed with the sodium bicarbonate cotransporter (NBC) in Xenopus oocytes.
2004,
Pubmed
,
Xenbase
Bonanno,
Intracellular pH regulation in fresh and cultured bovine corneal endothelium. I. Na+/H+ exchange in the absence and presence of HCO3-.
1992,
Pubmed
Bonanno,
Molecular mechanisms underlying the corneal endothelial pump.
2012,
Pubmed
Burckhardt,
Pathways of NH3/NH4+ permeation across Xenopus laevis oocyte cell membrane.
1992,
Pubmed
,
Xenbase
Damkier,
Molecular expression of SLC4-derived Na+-dependent anion transporters in selected human tissues.
2007,
Pubmed
Decoursey,
Voltage-gated proton channels and other proton transfer pathways.
2003,
Pubmed
DeCoursey,
The Voltage-Gated Proton Channel: A Riddle, Wrapped in a Mystery, inside an Enigma.
2015,
Pubmed
De-la-Rosa,
Currents through Hv1 channels deplete protons in their vicinity.
2016,
Pubmed
,
Xenbase
Desir,
Borate transporter SLC4A11 mutations cause both Harboyan syndrome and non-syndromic corneal endothelial dystrophy.
2007,
Pubmed
Diarra,
In situ calibration and [H+] sensitivity of the fluorescent Na+ indicator SBFI.
2001,
Pubmed
Donowitz,
SLC9/NHE gene family, a plasma membrane and organellar family of Na⁺/H⁺ exchangers.
2013,
Pubmed
Endeward,
Evidence that aquaporin 1 is a major pathway for CO2 transport across the human erythrocyte membrane.
2006,
Pubmed
,
Xenbase
Friedman,
CO2-stimulated NaCl absorption in the mouse renal cortical thick ascending limb of Henle. Evidence for synchronous Na +/H+ and Cl-/HCO3- exchange in apical plasma membranes.
1982,
Pubmed
Frommer,
Plant biology: Ping-pong with boron.
2002,
Pubmed
Gröger,
SLC4A11 prevents osmotic imbalance leading to corneal endothelial dystrophy, deafness, and polyuria.
2010,
Pubmed
Han,
Mice with a targeted disruption of Slc4a11 model the progressive corneal changes of congenital hereditary endothelial dystrophy.
2013,
Pubmed
Harper,
Diurnal variations in human corneal thickness.
1996,
Pubmed
Hebert,
Hypertonic cell volume regulation in mouse thick limbs. II. Na+-H+ and Cl(-)-HCO3- exchange in basolateral membranes.
1986,
Pubmed
Hemadevi,
Identification of mutations in the SLC4A11 gene in patients with recessive congenital hereditary endothelial dystrophy.
2008,
Pubmed
Hodson,
A near-zero membrane potential in transporting corneal endothelial cells of rabbit.
1989,
Pubmed
Hull,
Intracellular pH and glutathione levels in rabbit corneal endothelium following storage in moist chamber and MK medium.
1983,
Pubmed
Jacobs,
THE INFLUENCE OF AMMONIUM SALTS ON CELL REACTION.
1922,
Pubmed
Jalimarada,
Ion transport function of SLC4A11 in corneal endothelium.
2013,
Pubmed
Jiang,
Secondary regulatory volume increase conferred on Xenopus oocytes by expression of AE2 anion exchanger.
1997,
Pubmed
,
Xenbase
Kao,
Human SLC4A11-C functions as a DIDS-stimulatable H⁺(OH⁻) permeation pathway: partial correction of R109H mutant transport.
2015,
Pubmed
Li,
Transient formation of water-conducting states in membrane transporters.
2013,
Pubmed
Licsandru,
Salt-Excluding Artificial Water Channels Exhibiting Enhanced Dipolar Water and Proton Translocation.
2016,
Pubmed
Liskova,
Detailed assessment of renal function in a proband with Harboyan syndrome caused by a novel homozygous SLC4A11 nonsense mutation.
2015,
Pubmed
Loganathan,
Functional assessment of SLC4A11, an integral membrane protein mutated in corneal dystrophies.
2016,
Pubmed
,
Xenbase
Lopez,
Slc4a11 gene disruption in mice: cellular targets of sensorineuronal abnormalities.
2009,
Pubmed
Makani,
Endogenous alkaline transients boost postsynaptic NMDA receptor responses in hippocampal CA1 pyramidal neurons.
2007,
Pubmed
McAlear,
Electrogenic Na/HCO3 cotransporter (NBCe1) variants expressed in Xenopus oocytes: functional comparison and roles of the amino and carboxy termini.
2006,
Pubmed
,
Xenbase
Musa-Aziz,
Using fluorometry and ion-sensitive microelectrodes to study the functional expression of heterologously-expressed ion channels and transporters in Xenopus oocytes.
2010,
Pubmed
,
Xenbase
Musa-Aziz,
Concentration-dependent effects on intracellular and surface pH of exposing Xenopus oocytes to solutions containing NH3/NH4(+).
2009,
Pubmed
,
Xenbase
Myers,
Mouse Slc4a11 expressed in Xenopus oocytes is an ideally selective H+/OH- conductance pathway that is stimulated by rises in intracellular and extracellular pH.
2016,
Pubmed
,
Xenbase
Nakhoul,
Transport of NH(3)/NH in oocytes expressing aquaporin-1.
2001,
Pubmed
,
Xenbase
Nehrke,
H(OH), H(OH), H(OH): a holiday perspective. Focus on "Mouse Slc4a11 expressed in Xenopus oocytes is an ideally selective H+/OH- conductance pathway that is stimulated by rises in intracellular and extracellular pH".
2018,
Pubmed
,
Xenbase
Nguyen,
Bicarbonate, NBCe1, NHE, and carbonic anhydrase activity enhance lactate-H+ transport in bovine corneal endothelium.
2011,
Pubmed
Ogando,
SLC4A11 is an EIPA-sensitive Na(+) permeable pHi regulator.
2013,
Pubmed
Park,
NaBC1 is a ubiquitous electrogenic Na+ -coupled borate transporter essential for cellular boron homeostasis and cell growth and proliferation.
2004,
Pubmed
,
Xenbase
Parker,
Human BTR1, a new bicarbonate transporter superfamily member and human AE4 from kidney.
2001,
Pubmed
Parker,
The divergence, actions, roles, and relatives of sodium-coupled bicarbonate transporters.
2013,
Pubmed
Parker,
Cloning and characterization of novel human SLC4A8 gene products encoding Na+-driven Cl-/HCO3(-) exchanger variants NDCBE-A, -C, and -D.
2008,
Pubmed
,
Xenbase
Peña-Münzenmayer,
Ae4 (Slc4a9) is an electroneutral monovalent cation-dependent Cl-/HCO3- exchanger.
2016,
Pubmed
Romero,
The SLC4 family of bicarbonate (HCO₃⁻) transporters.
2013,
Pubmed
Swan,
Rabbit corneal hydration and the bicarbonate pump.
2004,
Pubmed
Thomas,
Hydrogen ion currents and intracellular pH in depolarized voltage-clamped snail neurones.
1982,
Pubmed
Vilas,
A biochemical framework for SLC4A11, the plasma membrane protein defective in corneal dystrophies.
2011,
Pubmed
Vilas,
Transmembrane water-flux through SLC4A11: a route defective in genetic corneal diseases.
2013,
Pubmed
,
Xenbase
Vithana,
Mutations in sodium-borate cotransporter SLC4A11 cause recessive congenital hereditary endothelial dystrophy (CHED2).
2006,
Pubmed
Wang,
Effects of Nt-truncation and coexpression of isolated Nt domains on the membrane trafficking of electroneutral Na+/HCO3- cotransporters.
2015,
Pubmed
,
Xenbase
Watsky,
Resting voltage measurements of the rabbit corneal endothelium using patch-current clamp techniques.
1991,
Pubmed
Weber,
Ion currents of Xenopus laevis oocytes: state of the art.
1999,
Pubmed
,
Xenbase
Zeuthen,
Water-transporting proteins.
2010,
Pubmed
Zhang,
Human SLC4A11 Is a Novel NH3/H+ Co-transporter.
2015,
Pubmed
