Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Methods Mol Biol
2016 Jan 01;1481:101-9. doi: 10.1007/978-1-4939-6393-5_11.
Show Gene links
Show Anatomy links
Reconstitution of the Cytoplasmic Regulation of the Wnt Signaling Pathway Using Xenopus Egg Extracts.
Hyde AS
,
Hang BI
.
???displayArticle.abstract???
The regulation of β-catenin turnover is the central mechanism governing activation of the Wnt signaling pathway. All components of the pathway are present in the early embryo of Xenopus laevis, and Xenopus egg extracts have been used to recapitulate complex biological reactions such as microtubule dynamics, DNA replication, chromatin assembly, and phases of the cell cycle. Herein, we describe a biochemical method for analyzing β-catenin degradation using radiolabeled and luciferase-fusion proteins in Xenopus egg extracts. We show that in such a biochemical system, cytoplasmic β-catenin degradation is regulated by soluble components of the Wnt pathway as well as small molecules.
Cselenyi,
LRP6 transduces a canonical Wnt signal independently of Axin degradation by inhibiting GSK3's phosphorylation of beta-catenin.
2008, Pubmed,
Xenbase
Cselenyi,
LRP6 transduces a canonical Wnt signal independently of Axin degradation by inhibiting GSK3's phosphorylation of beta-catenin.
2008,
Pubmed
,
Xenbase
Dupré,
A forward chemical genetic screen reveals an inhibitor of the Mre11-Rad50-Nbs1 complex.
2008,
Pubmed
,
Xenbase
Gao,
Casein kinase I phosphorylates and destabilizes the beta-catenin degradation complex.
2002,
Pubmed
,
Xenbase
Hang,
Screening for small molecule inhibitors of embryonic pathways: sometimes you gotta crack a few eggs.
2012,
Pubmed
,
Xenbase
Jernigan,
Gbetagamma activates GSK3 to promote LRP6-mediated beta-catenin transcriptional activity.
2010,
Pubmed
,
Xenbase
Kinzler,
Lessons from hereditary colorectal cancer.
1996,
Pubmed
Lee,
Physiological regulation of [beta]-catenin stability by Tcf3 and CK1epsilon.
2001,
Pubmed
,
Xenbase
Lee,
The roles of APC and Axin derived from experimental and theoretical analysis of the Wnt pathway.
2003,
Pubmed
,
Xenbase
Li,
Protein phosphatase 2A and its B56 regulatory subunit inhibit Wnt signaling in Xenopus.
2001,
Pubmed
,
Xenbase
MacDonald,
Wnt/beta-catenin signaling: components, mechanisms, and diseases.
2009,
Pubmed
,
Xenbase
Major,
Wilms tumor suppressor WTX negatively regulates WNT/beta-catenin signaling.
2007,
Pubmed
,
Xenbase
Saito-Diaz,
The way Wnt works: components and mechanism.
2013,
Pubmed
Salic,
Control of beta-catenin stability: reconstitution of the cytoplasmic steps of the wnt pathway in Xenopus egg extracts.
2000,
Pubmed
,
Xenbase
Salic,
Identifying small molecule inhibitors of the ubiquitin-proteasome pathway in Xenopus egg extracts.
2005,
Pubmed
,
Xenbase
Thorne,
A biochemical screen for identification of small-molecule regulators of the Wnt pathway using Xenopus egg extracts.
2011,
Pubmed
,
Xenbase
Thorne,
Small-molecule inhibition of Wnt signaling through activation of casein kinase 1α.
2010,
Pubmed
,
Xenbase
