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Structure, localization and transcriptional properties of two classes of retinoic acid receptor alpha fusion proteins in acute promyelocytic leukemia (APL): structural similarities with a new family of oncoproteins.
Kastner P
,
Perez A
,
Lutz Y
,
Rochette-Egly C
,
Gaub MP
,
Durand B
,
Lanotte M
,
Berger R
,
Chambon P
.
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Acute promyelocytic leukemia (APL) is due to a chromosomal t(15;17) translocation which involves a novel human gene, Myl, (also named PML) and the retinoic acid (RA) receptor alpha (RAR-alpha) gene. We report here the characterization of Myl and of the reciprocal MylRAR (PMLRAR) and RARMyl (RARPML) fusion transcripts which are found in two classes of APL patients. Myl displays similarities with a new family of proteins of which some members are fused to protooncogenes in the transforming proteins RFP-ret and T18. The speckled nuclear localization of Myl, as well as its sequence homology with the 52 kDa component of the RO/SSA ribonucleoprotein particle, suggest that Myl may be present in a ribonucleoprotein complex. In contrast to both Myl and RAR-alpha whose localization is essentially nuclear in the presence or absence of RA, MylRAR which is largely cytoplasmic in the absence of RA appears to be translocated to the nucleus in the presence of RA. Myl and MylRAR can associate in vitro and this association is mediated by a coiled coil in the Myl sequence. In vivo this association results in a colocalization of Myl and MylRAR which is identical to that of MylRAR alone. Studies of activation of transcription from the promoters of several RA target genes indicate that MylRARs have altered transcription activation properties when compared with RAR-alpha. Most notably, MylRAR represses markedly the activity of some RA target promoters in the absence of RA. Western blot analyses of patient samples show that MylRAR is expressed to a much higher level than wild type RAR-alpha originating from the normal allele. Taken together, these results suggest that MylRAR may interfere in a dominant manner with both Myl and RAR functions.
Alcalay,
Translocation breakpoint of acute promyelocytic leukemia lies within the retinoic acid receptor alpha locus.
1991, Pubmed
Alcalay,
Translocation breakpoint of acute promyelocytic leukemia lies within the retinoic acid receptor alpha locus.
1991,
Pubmed
Ben-Chetrit,
A 52-kD protein is a novel component of the SS-A/Ro antigenic particle.
1988,
Pubmed
Berg,
Zinc fingers and other metal-binding domains. Elements for interactions between macromolecules.
1990,
Pubmed
Borrow,
Molecular analysis of acute promyelocytic leukemia breakpoint cluster region on chromosome 17.
1990,
Pubmed
Brand,
Identification of a second human retinoic acid receptor.
1988,
Pubmed
Brand,
Characterization of a functional promoter for the human retinoic acid receptor-alpha (hRAR-alpha).
1990,
Pubmed
Breitman,
Induction of differentiation of the human promyelocytic leukemia cell line (HL-60) by retinoic acid.
1980,
Pubmed
Brockes,
Retinoids, homeobox genes, and limb morphogenesis.
1989,
Pubmed
Brunk,
Drosophila genes Posterior Sex Combs and Suppressor two of zeste encode proteins with homology to the murine bmi-1 oncogene.
1991,
Pubmed
Carmo-Fonseca,
In vivo detection of snRNP-rich organelles in the nuclei of mammalian cells.
1991,
Pubmed
Castaigne,
All-trans retinoic acid as a differentiation therapy for acute promyelocytic leukemia. I. Clinical results.
1990,
Pubmed
Cavener,
Eukaryotic start and stop translation sites.
1991,
Pubmed
Chan,
Molecular definition and sequence motifs of the 52-kD component of human SS-A/Ro autoantigen.
1991,
Pubmed
Chen,
The retinoic acid alpha receptor gene is frequently disrupted in its 5' part in Chinese patients with acute promyelocytic leukemia.
1991,
Pubmed
Chomienne,
Retinoic acid therapy for promyelocytic leukaemia.
1989,
Pubmed
Clarkson,
Retinoic acid in acute promyelocytic leukemia: the promise and the paradox.
1991,
Pubmed
Cleary,
Oncogenic conversion of transcription factors by chromosomal translocations.
1991,
Pubmed
de Thé,
The t(15;17) translocation of acute promyelocytic leukaemia fuses the retinoic acid receptor alpha gene to a novel transcribed locus.
1990,
Pubmed
de Thé,
The PML-RAR alpha fusion mRNA generated by the t(15;17) translocation in acute promyelocytic leukemia encodes a functionally altered RAR.
1991,
Pubmed
Evans,
The steroid and thyroid hormone receptor superfamily.
1988,
Pubmed
Forman,
Interactions among a subfamily of nuclear hormone receptors: the regulatory zipper model.
1990,
Pubmed
Freemont,
A novel cysteine-rich sequence motif.
1991,
Pubmed
Fu,
Factor required for mammalian spliceosome assembly is localized to discrete regions in the nucleus.
1990,
Pubmed
Gall,
Spliceosomes and snurposomes.
1991,
Pubmed
Garnier,
Analysis of the accuracy and implications of simple methods for predicting the secondary structure of globular proteins.
1978,
Pubmed
Gascuel,
A simple method for predicting the secondary structure of globular proteins: implications and accuracy.
1988,
Pubmed
Gaub,
Antibodies specific to the retinoic acid human nuclear receptors alpha and beta.
1989,
Pubmed
Gelman,
Herpes simplex virus immediate-early promoters are responsive to virus and cell trans-acting factors.
1987,
Pubmed
Giguere,
Identification of a receptor for the morphogen retinoic acid.
,
Pubmed
Glass,
Multiple cell type-specific proteins differentially regulate target sequence recognition by the alpha retinoic acid receptor.
1990,
Pubmed
Green,
A versatile in vivo and in vitro eukaryotic expression vector for protein engineering.
1988,
Pubmed
Green,
Nuclear receptors enhance our understanding of transcription regulation.
1988,
Pubmed
Green,
Human oestrogen receptor cDNA: sequence, expression and homology to v-erb-A.
,
Pubmed
Haupt,
Novel zinc finger gene implicated as myc collaborator by retrovirally accelerated lymphomagenesis in E mu-myc transgenic mice.
1991,
Pubmed
Huang,
Use of all-trans retinoic acid in the treatment of acute promyelocytic leukemia.
1988,
Pubmed
Itoh,
Protein heterogeneity in the human Ro/SSA ribonucleoproteins. The 52- and 60-kD Ro/SSA autoantigens are encoded by separate genes.
1991,
Pubmed
Jones,
The Saccharomyces cerevisiae RAD18 gene encodes a protein that contains potential zinc finger domains for nucleic acid binding and a putative nucleotide binding sequence.
1988,
Pubmed
Kakizuka,
Chromosomal translocation t(15;17) in human acute promyelocytic leukemia fuses RAR alpha with a novel putative transcription factor, PML.
1991,
Pubmed
Kastner,
Murine isoforms of retinoic acid receptor gamma with specific patterns of expression.
1990,
Pubmed
Lanotte,
NB4, a maturation inducible cell line with t(15;17) marker isolated from a human acute promyelocytic leukemia (M3).
1991,
Pubmed
Leroy,
Multiple isoforms of the mouse retinoic acid receptor alpha are generated by alternative splicing and differential induction by retinoic acid.
1991,
Pubmed
Leroy,
Mouse retinoic acid receptor alpha 2 isoform is transcribed from a promoter that contains a retinoic acid response element.
1991,
Pubmed
Loh,
Polymerase chain reaction with single-sided specificity: analysis of T cell receptor delta chain.
1989,
Pubmed
Longo,
Rearrangements and aberrant expression of the retinoic acid receptor alpha gene in acute promyelocytic leukemias.
1990,
Pubmed
Lutz,
The distribution of two hnRNP-associated proteins defined by a monoclonal antibody is altered in heat-shocked HeLa cells.
1988,
Pubmed
Mattei,
Mapping of the human retinoic acid receptor to the q21 band of chromosome 17.
1988,
Pubmed
Mendelsohn,
Developmental analysis of the retinoic acid-inducible RAR-beta 2 promoter in transgenic animals.
1991,
Pubmed
Miki,
Development of a highly efficient expression cDNA cloning system: application to oncogene isolation.
1991,
Pubmed
Mitchell,
Transcriptional regulation in mammalian cells by sequence-specific DNA binding proteins.
1989,
Pubmed
Nagpal,
Characterization of a potent varicella-zoster virus-encoded trans-repressor.
1991,
Pubmed
Pandolfi,
Structure and origin of the acute promyelocytic leukemia myl/RAR alpha cDNA and characterization of its retinoid-binding and transactivation properties.
1991,
Pubmed
Patarca,
rpt-1, an intracellular protein from helper/inducer T cells that regulates gene expression of interleukin 2 receptor and human immunodeficiency virus type 1.
1988,
Pubmed
Rochette-Egly,
Retinoic acid receptor gamma: specific immunodetection and phosphorylation.
1991,
Pubmed
Rowley,
15/17 translocation, a consistent chromosomal change in acute promyelocytic leukaemia.
1977,
Pubmed
Sawyers,
Leukemia and the disruption of normal hematopoiesis.
1991,
Pubmed
Schatz,
The V(D)J recombination activating gene, RAG-1.
1989,
Pubmed
Silver,
How proteins enter the nucleus.
1991,
Pubmed
Smith,
A retinoic acid response element is present in the mouse cellular retinol binding protein I (mCRBPI) promoter.
1991,
Pubmed
Tagawa,
Expression of novel DNA-binding protein with zinc finger structure in various tumor cells.
1990,
Pubmed
Takahashi,
Developmentally regulated expression of a human "finger"-containing gene encoded by the 5' half of the ret transforming gene.
1988,
Pubmed
Tasset,
Distinct classes of transcriptional activating domains function by different mechanisms.
1990,
Pubmed
Tora,
The human estrogen receptor has two independent nonacidic transcriptional activation functions.
1989,
Pubmed
Vallee,
Zinc fingers, zinc clusters, and zinc twists in DNA-binding protein domains.
1991,
Pubmed
van Lohuizen,
Sequence similarity between the mammalian bmi-1 proto-oncogene and the Drosophila regulatory genes Psc and Su(z)2.
1991,
Pubmed
van Lohuizen,
Identification of cooperating oncogenes in E mu-myc transgenic mice by provirus tagging.
1991,
Pubmed
Warrell,
Differentiation therapy of acute promyelocytic leukemia with tretinoin (all-trans-retinoic acid).
1991,
Pubmed
Zelent,
Differentially expressed isoforms of the mouse retinoic acid receptor beta generated by usage of two promoters and alternative splicing.
1991,
Pubmed
Zelent,
Cloning of murine alpha and beta retinoic acid receptors and a novel receptor gamma predominantly expressed in skin.
1989,
Pubmed
