Wang SK , Su HF , Gu YC , Lin SL , Tan JH , Huang ZS , Ou TM .

	Fig. 1. Structures of the G-quadruplex ligands used in this paper, the cationic porphyrin TMPyP4, the quindoline derivatives SYUIQ-05 and SYUIQ-FM05, and the isaindigotone derivative ISD-05.
	Fig. 4. The FRET melting curves of HTG-21 G-quadruplex in ionic buffer (A), dialysis buffer (B), cell-free system (C), control system (D), and Xenopus laevis egg extracts (E), with or without 0.2 μM or 0.4 μM TMPyP4, SYUIQ-05, SYUIQ-FM05, and ISD-05.
	Fig. 6. Effects of different compounds on the polymerase stop assay with HTG21 and its corresponding mutant sequence HTG21-mu in the cell free system, Xenopus laevis egg extract, and dialysis buffer mentioned before. All of the compounds were at 2.5 μM. Data represented means of six independent experiments with standard error.
	Fig. 2. (A), CD spectra of 5 μM HTG21 oligomer in non-ionic buffer (10 mM Tris–HCl buffer, pH 7.4), ionic buffer [10 mM Tris–HCl buffer (pH 7.4) containing 110 mM KCl], dialysis buffer, control system (dialysis buffer with 40% PEG), Xenopus laevis egg extract, and cell-free system (dialysis buffer with cell extract). (B), The FRET melting curves of HTG21G-quadruplex in ionic buffer, dialysis buffer, cell-free system, X. laevis egg extract, and control system.
	Fig. 3. Effects of different systems on the polymerase stop assay with HTG21 and its corresponding mutant sequence HTG21-mu in a normal PCR reaction buffer, the cell free system, Xenopus laevis egg extract, dialysis buffer, dialysis buffer A, and control system mentioned before. Data represented means of six independent experiments with standard error.
	Fig. 5. Fitted curves obtained by MST measurements of 5’-FAM-labeled HTG-21 with ligands diluted 1:2 from 20 μM for 14 times.
	Fig. 7. Gel electrophoresis of HTG21 in ionic buffer [10 mM Tris–HCl buffer (pH 7.4) containing 110 mM KCl], cell free system, Xenopus laevis egg extract, and control system (the dialysis buffer containing 40% PEG).

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