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XB-ART-54905
Cold Spring Harb Protoc 2019 Jun 03;20196:. doi: 10.1101/pdb.prot103481.
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Calculating the Degradation Rate of Individual Proteins Using Xenopus Extract Systems.



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The Xenopus extract system has been used extensively as a simple, quick, and robust method for assessing the stability of proteins against proteasomal degradation. In this protocol, methods are provided for assessing the half-life of in vitro translated radiolabeled proteins using Xenopus egg or embryo extracts.

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Species referenced: Xenopus laevis