Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Methods Mol Biol
2018 Jan 01;1801:247-263. doi: 10.1007/978-1-4939-7902-8_20.
Show Gene links
Show Anatomy links
Role of Thyroid Hormone Receptor in Amphibian Development.
Fu L
,
Wen L
.
???displayArticle.abstract???
The amphibian Xenopus laevis has long been used as a model for studying vertebrate cell and developmental biology largely due to the easiness to manipulate this system in vivo and in vitro. While most of the developmental studies have been on Xenopus embryogenesis, considerable efforts have been made to understand its metamorphosis, a process mimicking postembryonic development in mammals when many organs mature into their adult forms in the presence of high levels of thyroid hormone (T3). Amphibian metamorphosis is totally dependent on T3 and offers a number of advantages for experimental analyses compared to the late stage, uterus-enclosed mammalian embryos. Earlier studies on metamorphosis in Xenopus laevis have revealed dual functions of T3 receptors (TR) during premetamorphic development and metamorphosis as well as important roles of TR-interacting corepressors and coactivators during these two periods, respectively. The development of gene-editing technologies that functions in amphibians in recent years has made possible for the first time to study function of endogenous TRs, especially in the highly related diploid anuran species Xenopus tropicalis. Here, we first review the current mechanistic understanding of the regulation of metamorphosis by T3 and TR, and then describe a detailed method to use TALEN to knock out TRα for studying its role in gene regulation by T3 in vivo and Xenopus development.
Amaya,
A method for generating transgenic frog embryos.
1999, Pubmed,
Xenbase
Amaya,
A method for generating transgenic frog embryos.
1999,
Pubmed
,
Xenbase
Blitz,
Biallelic genome modification in F(0) Xenopus tropicalis embryos using the CRISPR/Cas system.
2013,
Pubmed
,
Xenbase
Buchholz,
A dominant-negative thyroid hormone receptor blocks amphibian metamorphosis by retaining corepressors at target genes.
2003,
Pubmed
,
Xenbase
Buchholz,
Molecular and developmental analyses of thyroid hormone receptor function in Xenopus laevis, the African clawed frog.
2006,
Pubmed
,
Xenbase
Buchholz,
Transgenic analysis reveals that thyroid hormone receptor is sufficient to mediate the thyroid hormone signal in frog metamorphosis.
2004,
Pubmed
,
Xenbase
Cermak,
Efficient design and assembly of custom TALEN and other TAL effector-based constructs for DNA targeting.
2011,
Pubmed
Choi,
Unliganded thyroid hormone receptor α regulates developmental timing via gene repression in Xenopus tropicalis.
2015,
Pubmed
,
Xenbase
Choi,
Growth, Development, and Intestinal Remodeling Occurs in the Absence of Thyroid Hormone Receptor α in Tadpoles of Xenopus tropicalis.
2017,
Pubmed
,
Xenbase
Dahlem,
Simple methods for generating and detecting locus-specific mutations induced with TALENs in the zebrafish genome.
2012,
Pubmed
Das,
Multiple thyroid hormone-induced muscle growth and death programs during metamorphosis in Xenopus laevis.
2002,
Pubmed
,
Xenbase
Davis,
Membrane receptors mediating thyroid hormone action.
2005,
Pubmed
Davis,
Nongenomic actions of thyroid hormone.
1996,
Pubmed
Doyon,
Enhancing zinc-finger-nuclease activity with improved obligate heterodimeric architectures.
2011,
Pubmed
Evans,
The steroid and thyroid hormone receptor superfamily.
1988,
Pubmed
Fu,
Novel double promoter approach for identification of transgenic animals: A tool for in vivo analysis of gene function and development of gene-based therapies.
2002,
Pubmed
,
Xenbase
Fu,
A simple and efficient method to visualize and quantify the efficiency of chromosomal mutations from genome editing.
2016,
Pubmed
,
Xenbase
Guigon,
Novel non-genomic signaling of thyroid hormone receptors in thyroid carcinogenesis.
2009,
Pubmed
Guo,
Efficient RNA/Cas9-mediated genome editing in Xenopus tropicalis.
2014,
Pubmed
,
Xenbase
Hasebe,
Epithelial-connective tissue interactions induced by thyroid hormone receptor are essential for adult stem cell development in the Xenopus laevis intestine.
2011,
Pubmed
,
Xenbase
Hiroi,
Rapid nongenomic actions of thyroid hormone.
2006,
Pubmed
Hisano,
Quantitative assay for TALEN activity at endogenous genomic loci.
2013,
Pubmed
Lazar,
Thyroid hormone receptors: multiple forms, multiple possibilities.
1993,
Pubmed
Lei,
Generation of gene disruptions by transcription activator-like effector nucleases (TALENs) in Xenopus tropicalis embryos.
2013,
Pubmed
,
Xenbase
Lei,
Efficient targeted gene disruption in Xenopus embryos using engineered transcription activator-like effector nucleases (TALENs).
2012,
Pubmed
,
Xenbase
Nakajima,
Dual mechanisms governing muscle cell death in tadpole tail during amphibian metamorphosis.
2003,
Pubmed
,
Xenbase
Nakayama,
Simple and efficient CRISPR/Cas9-mediated targeted mutagenesis in Xenopus tropicalis.
2013,
Pubmed
,
Xenbase
Puzianowska-Kuznicka,
Both thyroid hormone and 9-cis retinoic acid receptors are required to efficiently mediate the effects of thyroid hormone on embryonic development and specific gene regulation in Xenopus laevis.
1997,
Pubmed
,
Xenbase
Qiu,
Mutation detection using Surveyor nuclease.
2004,
Pubmed
Sachs,
Dual functions of thyroid hormone receptors during Xenopus development.
2000,
Pubmed
,
Xenbase
Sachs,
Unliganded thyroid hormone receptor function: amphibian metamorphosis got TALENs.
2015,
Pubmed
,
Xenbase
Schreiber,
Tadpole skin dies autonomously in response to thyroid hormone at metamorphosis.
2003,
Pubmed
,
Xenbase
Schreiber,
Diverse developmental programs of Xenopus laevis metamorphosis are inhibited by a dominant negative thyroid hormone receptor.
2001,
Pubmed
,
Xenbase
Sedlak,
Digital detection of endonuclease mediated gene disruption in the HIV provirus.
2016,
Pubmed
Shi,
Thyroid hormone receptor actions on transcription in amphibia: The roles of histone modification and chromatin disruption.
2012,
Pubmed
Shi,
Tadpole competence and tissue-specific temporal regulation of amphibian metamorphosis: roles of thyroid hormone and its receptors.
1996,
Pubmed
,
Xenbase
Tata,
Gene expression during metamorphosis: an ideal model for post-embryonic development.
1993,
Pubmed
Tsai,
Molecular mechanisms of action of steroid/thyroid receptor superfamily members.
1994,
Pubmed
Vize,
Assays for gene function in developing Xenopus embryos.
1991,
Pubmed
,
Xenbase
Vouillot,
Comparison of T7E1 and surveyor mismatch cleavage assays to detect mutations triggered by engineered nucleases.
2015,
Pubmed
,
Xenbase
Wang,
Developmental regulation and function of thyroid hormone receptors and 9-cis retinoic acid receptors during Xenopus tropicalis metamorphosis.
2008,
Pubmed
,
Xenbase
Wang,
Targeted gene disruption in Xenopus laevis using CRISPR/Cas9.
2015,
Pubmed
,
Xenbase
Wen,
Unliganded thyroid hormone receptor α controls developmental timing in Xenopus tropicalis.
2015,
Pubmed
,
Xenbase
Wen,
Histone methyltransferase Dot1L plays a role in postembryonic development in Xenopus tropicalis.
2015,
Pubmed
,
Xenbase
Wen,
Thyroid Hormone Receptor α Controls Developmental Timing and Regulates the Rate and Coordination of Tissue-Specific Metamorphosis in Xenopus tropicalis.
2017,
Pubmed
,
Xenbase
Wong,
Coordinated regulation of and transcriptional activation by Xenopus thyroid hormone and retinoid X receptors.
1995,
Pubmed
,
Xenbase
Wu,
Raising Xenopus in the laboratory.
1991,
Pubmed
,
Xenbase
Yaoita,
A correlation of thyroid hormone receptor gene expression with amphibian metamorphosis.
1990,
Pubmed
,
Xenbase
Yen,
Physiological and molecular basis of thyroid hormone action.
2001,
Pubmed
Yen,
Unliganded TRs regulate growth and developmental timing during early embryogenesis: evidence for a dual function mechanism of TR action.
2015,
Pubmed
,
Xenbase
