Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
???displayArticle.abstract???
When temperatures plummet below 0 °C, wood frogs (Rana sylvatica) can endure the freezing of up to ~ 65% of their body water in extracellular ice masses, displaying no measurable brain activity, no breathing, no movement, and a flat-lined heart. To aid survival, frogs retreat into a state of suspended animation characterized by global suppression of metabolic functions and reprioritization of energy usage to essential survival processes that is elicited, in part, by the regulatory controls of microRNAs. The present study is the first to investigate miRNA biogenesis and regulation in the brain of a freeze tolerant vertebrate. Indeed, proper brain function and adaptations to environmental stimuli play a crucial role in coordinating stress responses. Immunoblotting of miRNA biogenesis factors illustrated an overall reduction in the majority of these processing proteins suggesting a potential suppression of miRNA maturation over the freeze-thaw cycle. This was coupled with a large-scale RT-qPCR analysis of relative expression levels of 113 microRNA species in the brains of control, 24 h frozen, and 8 h thawed R. sylvatica. Of the 41 microRNAs differentially regulated during freezing and thawing, only two were significantly upregulated. Bioinformatic target enrichment of the downregulated miRNAs, performed at the low temperatures experienced during freezing and thawing, predicted their involvement in the potential activation of various neuroprotective processes such as synaptic signaling, intracellular signal transduction, and anoxia/ischemia injury protection. The predominantly downregulated microRNA fingerprint identified herein suggests a microRNA-mediated cryoprotective mechanism responsible for maintaining neuronal functions and facilitating successful whole brain freezing and thawing.
Abe,
MicroRNAs and neurodegeneration: role and impact.
2013, Pubmed
Abe,
MicroRNAs and neurodegeneration: role and impact.
2013,
Pubmed
Aguilar,
Freeze-responsive regulation of MEF2 proteins and downstream gene networks in muscles of the wood frog, Rana sylvatica.
2017,
Pubmed
Aguilar,
Regulation of SMAD transcription factors during freezing in the freeze tolerant wood frog, Rana sylvatica.
2016,
Pubmed
Akhtar,
Bcl-2 family regulation of neuronal development and neurodegeneration.
2004,
Pubmed
Bansal,
MicroRNA regulation in heart and skeletal muscle over the freeze-thaw cycle in the freeze tolerant wood frog.
2016,
Pubmed
Bartel,
MicroRNAs: target recognition and regulatory functions.
2009,
Pubmed
Biggar,
High-throughput amplification of mature microRNAs in uncharacterized animal models using polyadenylated RNA and stem-loop reverse transcription polymerase chain reaction.
2014,
Pubmed
Biggar,
MicroRNA regulation in extreme environments: differential expression of microRNAs in the intertidal snail Littorina littorea during extended periods of freezing and anoxia.
2012,
Pubmed
Biggar,
The emerging roles of microRNAs in the molecular responses of metabolic rate depression.
2011,
Pubmed
Biggar,
MicroRNA regulation below zero: differential expression of miRNA-21 and miRNA-16 during freezing in wood frogs.
2009,
Pubmed
Biggar,
Low-temperature microRNA expression in the painted turtle, Chrysemys picta during freezing stress.
2015,
Pubmed
Biggar,
Insight into temperature-dependent microRNA function in mammalian hibernators: Perspectives on cold-influenced microRNA/target interaction.
2014,
Pubmed
Bohnsack,
Exportin 5 is a RanGTP-dependent dsRNA-binding protein that mediates nuclear export of pre-miRNAs.
2004,
Pubmed
,
Xenbase
Cai,
Upregulation of a novel gene by freezing exposure in the freeze-tolerant wood frog (Rana sylvatica).
1997,
Pubmed
Chen,
Large-scale identification and comparative analysis of miRNA expression profile in the respiratory tree of the sea cucumber Apostichopus japonicus during aestivation.
2014,
Pubmed
Chung,
miR-192 mediates TGF-beta/Smad3-driven renal fibrosis.
2010,
Pubmed
Costanzo,
Physiological responses of freeze-tolerant and -intolerant frogs: clues to evolution of anuran freeze tolerance.
1993,
Pubmed
Costanzo,
Physiological ecology of overwintering in hatchling turtles.
2008,
Pubmed
Dawson,
Free-radical first responders: the characterization of CuZnSOD and MnSOD regulation during freezing of the freeze-tolerant North American wood frog, Rana sylvatica.
2015,
Pubmed
Dieni,
Protein kinase C in the wood frog, Rana sylvatica: reassessing the tissue-specific regulation of PKC isozymes during freezing.
2014,
Pubmed
Ebert,
Roles for microRNAs in conferring robustness to biological processes.
2012,
Pubmed
Fukunaga,
Dicer partner proteins tune the length of mature miRNAs in flies and mammals.
2012,
Pubmed
Giniatullin,
SNAP25 is a pre-synaptic target for the depressant action of reactive oxygen species on transmitter release.
2006,
Pubmed
Godlewski,
microRNA-451: A conditional switch controlling glioma cell proliferation and migration.
2010,
Pubmed
Greenway,
Activation of mitogen-activated protein kinases during natural freezing and thawing in the wood frog.
2000,
Pubmed
Ha,
Regulation of microRNA biogenesis.
2014,
Pubmed
Hadj-Moussa,
The hibernating South American marsupial, Dromiciops gliroides, displays torpor-sensitive microRNA expression patterns.
2016,
Pubmed
Hartl,
Molecular chaperones in protein folding and proteostasis.
2011,
Pubmed
Holmstrup,
The ins and outs of water dynamics in cold tolerant soil invertebrates.
2014,
Pubmed
Hou,
Semaphorin3A elevates vascular permeability and contributes to cerebral ischemia-induced brain damage.
2015,
Pubmed
Huang,
Biological functions of microRNAs: a review.
2011,
Pubmed
Jaskiewicz,
Role of Dicer in posttranscriptional RNA silencing.
2008,
Pubmed
Jiang,
The complete mitochondrial genome of western painted turtle, Chrysemys picta bellii (Chrysemys, Emydidae).
2016,
Pubmed
Lee,
The role of PACT in the RNA silencing pathway.
2006,
Pubmed
Lee,
TRBP alters human precursor microRNA processing in vitro.
2012,
Pubmed
Leung,
MicroRNA functions in stress responses.
2010,
Pubmed
Luu,
Dehydration triggers differential microRNA expression in Xenopus laevis brain.
2015,
Pubmed
,
Xenbase
Lyons,
Identification and profiling of miRNAs in the freeze-avoiding gall moth Epiblema scudderiana via next-generation sequencing.
2015,
Pubmed
Lyons,
CryomiRs: towards the identification of a cold-associated family of microRNAs.
2013,
Pubmed
Ma,
Inhibition of microRNA-210 provides neuroprotection in hypoxic-ischemic brain injury in neonatal rats.
2016,
Pubmed
Macfarlane,
MicroRNA: Biogenesis, Function and Role in Cancer.
2010,
Pubmed
Moon,
Inhibition of microRNA-181 reduces forebrain ischemia-induced neuronal loss.
2013,
Pubmed
Naicker,
Identification of chaperones in freeze tolerance in Saccharomyces cerevisiae.
2012,
Pubmed
O'Carroll,
General principals of miRNA biogenesis and regulation in the brain.
2013,
Pubmed
Okada,
A high-resolution structure of the pre-microRNA nuclear export machinery.
2009,
Pubmed
Pei,
Extracellular HSP60 triggers tissue regeneration and wound healing by regulating inflammation and cell proliferation.
2016,
Pubmed
Ramaglia,
Time-dependent expression of heat shock proteins 70 and 90 in tissues of the anoxic western painted turtle.
2004,
Pubmed
Ryan,
Temporal profiling of gene networks associated with the late phase of long-term potentiation in vivo.
2012,
Pubmed
Sacconi,
miR-204 targets Bcl-2 expression and enhances responsiveness of gastric cancer.
2012,
Pubmed
Schmittgen,
Analyzing real-time PCR data by the comparative C(T) method.
2008,
Pubmed
Shannon,
Cytoscape: a software environment for integrated models of biomolecular interaction networks.
2003,
Pubmed
Shen,
EGFR modulates microRNA maturation in response to hypoxia through phosphorylation of AGO2.
2013,
Pubmed
Storey,
Tribute to P. L. Lutz: putting life on 'pause'--molecular regulation of hypometabolism.
2007,
Pubmed
Storey,
Molecular Physiology of Freeze Tolerance in Vertebrates.
2017,
Pubmed
Storey,
Life in a frozen state: adaptive strategies for natural freeze tolerance in amphibians and reptiles.
1990,
Pubmed
Storey,
Metabolic rate depression in animals: transcriptional and translational controls.
2004,
Pubmed
Storey,
Regulation of hypometabolism: insights into epigenetic controls.
2015,
Pubmed
Storey,
Reptile freeze tolerance: metabolism and gene expression.
2006,
Pubmed
Strimbeck,
Extreme low temperature tolerance in woody plants.
2015,
Pubmed
Sullivan,
Environmental stress responsive expression of the gene li16 in Rana sylvatica, the freeze tolerant wood frog.
2012,
Pubmed
Szklarczyk,
STRING v10: protein-protein interaction networks, integrated over the tree of life.
2015,
Pubmed
Tang,
Acetylation of drosha on the N-terminus inhibits its degradation by ubiquitination.
2013,
Pubmed
Tian,
MicroRNA miR-451 downregulates the PI3K/AKT pathway through CAB39 in human glioma.
2012,
Pubmed
Wang,
Quantitative functions of Argonaute proteins in mammalian development.
2012,
Pubmed
Winter,
Many roads to maturity: microRNA biogenesis pathways and their regulation.
2009,
Pubmed
Wu,
Up-regulation of acidic ribosomal phosphoprotein P0 in response to freezing or anoxia in the freeze tolerant wood frog, Rana sylvatica.
2005,
Pubmed
Wu,
Cold acclimation-induced up-regulation of the ribosomal protein L7 gene in the freeze tolerant wood frog, Rana sylvatica.
2008,
Pubmed
,
Xenbase
Wu,
Temperature dependence of thermodynamic properties for DNA/DNA and RNA/DNA duplex formation.
2002,
Pubmed
Xie,
MicroRNA‑451 protects against cardiomyocyte anoxia/reoxygenation injury by inhibiting high mobility group box 1 expression.
2016,
Pubmed
Xu,
Post-stroke treatment with miR-181 antagomir reduces injury and improves long-term behavioral recovery in mice after focal cerebral ischemia.
2015,
Pubmed
Yan-Chun,
MicroRNA-192-5p Promote the Proliferation and Metastasis of Hepatocellular Carcinoma Cell by Targeting SEMA3A.
2017,
Pubmed
Ye,
The effect of central loops in miRNA:MRE duplexes on the efficiency of miRNA-mediated gene regulation.
2008,
Pubmed
Yi,
Exportin-5 mediates the nuclear export of pre-microRNAs and short hairpin RNAs.
2003,
Pubmed
Yu,
miR-451 protects against erythroid oxidant stress by repressing 14-3-3zeta.
2010,
Pubmed
Zhang,
RBioplot: an easy-to-use R pipeline for automated statistical analysis and data visualization in molecular biology and biochemistry.
2016,
Pubmed
