Dong C , Paudel S , Amoh NY , Saha MS .

R15 HD077624 NICHD NIH HHS , R15 HD096415 NICHD NIH HHS

             gene expression

	Fig. 1. Whole mount (A–D) and histological (A′-D‴) expression of trpv1 transcripts in developing X. laevis embryos. (A–B) dorsal view, (C–D) lateral view, anterior to the right for all whole mount embryos; dorsal to the top for all histology images. (A, A′, A′) stage 20 (late neurula stage); (B, B′, B′) stage 25 (early tailbud stage); (C, C′, C′) stage 30 (late tailbud stage); (D, D′, D′, D‴) stage 35 (swimming tadpole stage). Arrows indicate regions of gene expression (nt, nural tube; sc, spinal cord; tg, trigeminal ganglia). Dashed lines represent positions of corresponding sections. Scale bars = 250 μm.
	Fig. 2. Whole mount (A–F) and histological (A′-F⁗) expression of trpv2 transcripts in X. laevis embryos. Lateral view for all whole mount embryos, animal pole to the top (A–C), anterior to the right (D–F); dorsal to the top for all histology images. (A, A′) unfertilized egg; (B, B′) stage 5 (16-cell stage); (C, C′) stage 8 (mid-blastula stage); (D, D′, D′) stage 25 (early tailbud stage); (E, E′, E′, E‴, E⁗;) stage 30 (late tailbud stage); (F, F′, F′, F‴, F⁗) stage 35 (swimming tadpole stage). Arrows indicate regions of gene expression (a, animal pole; cg, cement gland; nt, neural tube; op, optic vesicle; ot, otic vesicle; tg, trigeminal ganglia; sc, spinal cord). Dashed lines represent positions of corresponding sections. Scale bars = 250 μm.
	Fig. 3. Whole mount (A–G) and histological (A′-G′) expression of trpv4 mRNA expression in X. laevis embryos. Lateral view for all whole mount embryos, animal pole to the top (A–D), anterior to the right (E–G); dorsal to the top for all histology images. (A, A′) unfertilized egg; (B, B′) stage 5 (16-cell stage); (C, C′) stage 8 (mid-blastula stage); (D, C′) stage 10 (early gastrula stage); (E, E′) stage 20 (late neurula stage); (F, F′, F′) stage 30 (late tailbud stage); (G, G′, G′) stage 35 (swimming tadpole stage). Arrows indicate regions of gene expression (a, animal pole; nc, notochord; np, nasal placode; tb, tailbud). Dashed lines represent positions of corresponding sections. Scale bars = 250 μm.
	Fig. 4. Whole mount (A–E) and histological (A′-E′) expression of trpv5 transcripts in developing X. laevis embryos. (A) dorsal view, (B–E) lateral view, anterior to the right for all whole mount embryos; dorsal to the top for all histology images. (A, A′) stage 15 (mid-neurula stage); (B, B′, B′) stage 20 (late neurula stage); (C, C′, C′) stage 25 (early tailbud stage); (D, D′, D′) stage 30 (late tailbud stage); (E, E′) stage 35 (swimming tadpole stage). Arrows indicate regions of gene expression (ba, branchial arches; ep, epidermis; pd, proctodeum). Dashed lines represent positions of corresponding sections. Scale bars = 250 μm.
	Fig. 5. Whole mount (A–E) and histological (A′-E′) expression of trpv6 transcripts in X. laevis embryos. Lateral view for all whole mount embryos, anterior to the right; dorsal to the top for all histology images. (A, A′) stage 15 (mid-neurula stage); (B, B′) stage 20 (late neurula stage); (C, C′) stage 25 (early tailbud stage); (D, D′) stage 30 (late tailbud stage); (E, E′) stage 35 (swimming tadpole stage). Arrows indicate regions of gene expression (ep, epidermis). Dashed lines represent positions of corresponding sections. Scale bars = 250 μm.

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