Ratzan W et al. (2019), The voltage sensing phosphatase (VSP) localizes...

XB-ART-55845

PLoS One 2019 Jan 01;144:e0209056. doi: 10.1371/journal.pone.0209056.

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The voltage sensing phosphatase (VSP) localizes to the apical membrane of kidney tubule epithelial cells.

Ratzan W , Rayaprolu V , Killian SE , Bradley R , Kohout SC .

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Voltage-sensing phosphatases (VSPs) are transmembrane proteins that couple changes in membrane potential to hydrolysis of inositol signaling lipids. VSPs catalyze the dephosphorylation of phosphatidylinositol phosphates (PIPs) that regulate diverse aspects of cell membrane physiology including cell division, growth and migration. VSPs are highly conserved among chordates, and their RNA transcripts have been detected in the adult and embryonic stages of frogs, fish, chickens, mice and humans. However, the subcellular localization and biological function of VSP remains unknown. Using reverse transcriptase-PCR (RT-PCR), we show that both Xenopus laevis VSPs (Xl-VSP1 and Xl-VSP2) mRNAs are expressed in early embryos, suggesting that both Xl-VSPs are involved in early tadpole development. To understand which embryonic tissues express Xl-VSP mRNA, we used in situ hybridization (ISH) and found Xl-VSP mRNA in both the brain and kidney of NF stage 32-36 embryos. By Western blot analysis with a VSP antibody, we show increasing levels of Xl-VSP protein in the developing embryo, and by immunohistochemistry (IHC), we demonstrate that Xl-VSP protein is specifically localized to the apical membrane of both embryonic and adult kidney tubules. We further characterized the catalytic activity of both Xl-VSP homologs and found that while Xl-VSP1 catalyzes 3- and 5-phosphate removal, Xl-VSP2 is a less efficient 3-phosphatase with different substrate specificity. Our results suggest that Xl-VSP1 and Xl-VSP2 serve different functional roles and that VSPs are an integral component of voltage-dependent PIP signaling pathways during vertebrate kidney tubule development and function.

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Species referenced: Xenopus laevis
Genes referenced: cdh17 pax8
GO keywords: cell growth [+]

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	Fig 1. X. laevis embryonic stages show VSP mRNA transcripts expression.(A-B) Semi-quantitative RT-PCR (sqRT-PCR) of a panel of X. laevis embryos (NF stage 12â40) using PCR primers specific for Xl-VSP1 (A) and Xl-VSP2 (B). Both Xl-VSP1 and Xl-VSP2 transcripts appear to accumulate by stage 36. No bands were seen without reverse transcriptase (bottom). All cDNAs were made with equal amounts of total RNA as determined by spectrophotometry and confirmed by agarose gel electrophoresis to visualize ribosomal RNA bands (S1 Fig). sqRT-PCR was repeated at least two times with at least two different embryonic cohorts. The expected PCR amplicon sizes are 389 bp for Xl-VSP1 and 478 bp for Xl-VSP2. Shown are representative gels.
	Fig 2. VSP mRNA is located in the pronephros and brain of X. laevis embryos.(A) In situ hybridization (ISH) of whole-mount NF stage 32 embryos. An anti-sense probe against Xl-VSPs shows Xl-VSP transcript in the proximal pronephritic field (black arrowhead) and brain (white arrowhead) of the embryos. This probe does not distinguish between Xl-VSP1 and Xl-VSP2 mRNAs because of the similarity between the two transcripts at the nucleotide level (93%). (B) No staining was observed by ISH in a sibling embryo with a sense control probe. ISH was repeated four times with four different embryonic cohorts. Shown are representative embryos.
	Fig 3. X. laevis tissues and embryos show VSP protein expression.(A) Western blot validation of N432/21 anti-VSP in X. laevis oocytes injected with cRNA for Dr-VSP (Dr), FLAG-Ci-VSP (Ci), Xl-VSP1 (Xl1), Xl-VSP2 (Xl2), Xt-VSP (Xt), or left un-injected (U). All VSPs tested were recognized by the antibody. Un-injected oocytes (U) display no band. Dr-VSP, Xl-VSP1, Xl-VSP2 and Xt-VSP have a predicted MW of 58 kDa while FLAG-Ci-VSP has a predicted MW of 66 kDa. The slight difference in electrophoretic mobility between VSPs and their predicted MWs and the nature of the double band for Xl-VSP1 and Xl-VSP2 (as seen in panels A and C) has not been determined. These results show that anti-VSP N432/21 is specific for VSP and cross-reacts with VSPs from multiple species. (B) X. laevis zygotes were injected with either a control morpholino, an Xl-VSP2 morpholino or left un-injected. (top) Western blot analysis of equal amounts of lysates from embryos at stage 42 shows a band at the predicted MW for Xl-VSPs (58 kDa) for un-injected and control embryos and no band for the Xl2 morpholino-injected embryos. (bottom) Blots were stripped and re-probed with anti-actin for a loading control. (C) Western blot analysis of X. laevis tissues. Lysates from adult kidney (K, 3 Î¼g), testis (T, 30 Î¼g), and brain (B, 10 Î¼g) were run against lysates from oocytes injected with RNAs for Xl-VSP1 (Xl1) and Xl-VSP2 (Xl2) and analyzed by Western blot with anti-VSP. A single band of approximately the correct MW (58 kDa) was observed in the tissue lysates, indicating the presence of Xl-VSP protein in all tissues tested. (D) Western blot analysis of X. laevis embryos. Lysates from NF stage 12â40 embryos (30 Î¼g each) were run against a lysate from adult kidney (K, 5 Î¼g). Blots were probed either with anti-VSP (top) or anti-actin (bottom) as a loading control (predicted MW 42 kDa). A weak band (potentially corresponding to Xl-VSP1) is present only at early embryonic stages 12â20 (red arrowheads), whereas a slightly slower-migrating band (potentially corresponding to Xl-VSP2) accumulates at later embryonic stages 36â40. The difference between the kidney and embryo lysate MWs for both VSP and actin may reflect a different degree of post-translational modification in embryos versus adult X. laevis. Lysates, gels and blots were repeated three times with either three different adults or three different embryonic cohorts. Shown are representative gels for each.
	Fig 4. Xl-VSP protein is expressed on the lumenal surface of embryonic pronephroi.NF stage 42 Cdh17:GFP X. laevis embryos were sectioned and stained to test for VSP protein. Kidney tubules were identified by the presence of the Cdh17:GFP transgene (B, B'). Sections stained with anti-VSP (A) showed fluorescence on the lumenal surface (corresponding to the apical membrane) of the proximal kidney tubule cells (outlined in white). Anti-VSP staining was only observed in the presence of anti-VSP (A-F) and not in secondary antibody alone control sections (A'-F'). Panels (A, A') anti-VSP or no anti-VSP control; (B, B') GFP transgene; (C, C') fluorescence signal overlay of A, B and E; (D, D') bright field images; (E, E') Hoechst 33342 (to mark nuclei); (F, F') signal overlay of A, B, D and E. IHC was repeated three times with three different embryonic cohorts. Shown are representative sections. Scale bar = 25 Î¼m.
	Fig 5. Xl-VSP protein is expressed on the lumenal surface of adult kidney tubule epithelial cells.(A) Adult kidney sections were stained with anti-VSP and anti-mouse IgG-Alexa 488. Xl-VSP staining is observed on the lumenal surface (marked with arrowheads) of the kidney tubules (two outlined in white). This surface corresponds to the apical membrane and not the basolateral membrane of the epithelial cells. (B) Apical membrane staining was not seen on a consecutive section in the absence of anti-VSP primary antibody. IHC was repeated on sections from kidneys of three different adult males. Shown are representative sections. Scale bar = 25 Î¼m.
	Fig 6. Xl-VSP1 and Xl-VSP2 function as voltage-regulated 5- and 3-phosphatases.(A) Schematics of the known VSP reactions. (B) Cartoon representation of fTAPP binding and release with increasing and decreasing PI(3,4)P2 concentrations. The binding of fTAPP to PI(3,4)P2 results in a conformational change that increases the FRET signal. Similarly, a reduction of PI(3,4)P2 results in a decrease in the FRET signal. Similar binding and release occurs with the fPLC sensor when PI(4,5)P2 concentrations are changed. (C) Oocytes were injected with fTAPP and either Xl-VSP1, Xl-VSP2 or Xl-VSP2 C301S (Xl2-CS), a catalytically inactivated protein. (left) Averaged fTAPP FRET traces over time during a voltage step from a holding potential of -100 to +160 mV. The FRET signal increases and decreases in the same pulse for Xl-VSP1 while it only increases for Xl-VSP2. (right) FRET measurements were tested at several voltages and the ÎF/F fTAPP FRET ratio was plotted versus the voltage (RV). The FRET increase (net 5-phosphatase reaction, solid line, closed symbols) was plotted by subtracting the baseline from the peak ÎF/F FRET ratio. The FRET decrease (net 3-phosphatase reaction, dashed line, open symbols) was plotted by subtracting the peak ÎF/F FRET ratio from the ÎF/F FRET ratio at the end of the voltage step. Xl-VSP1 shows robust activity as both a 3- and 5- phosphatase while Xl-VSP2 functions as a 5-phosphatase. (D) Oocytes were injected with fPLC and either Xl-VSP1, Xl-VSP2 or Xl2-CS. (left) Averaged fPLC FRET traces over time during a voltage step from a holding potential of -100 to +160 mV for fPLC co-expressed with either Xl-VSP1 or Xl-VSP2. The kinetics of activation are significantly faster for Xl-VSP1 than for Xl-VSP2. (right) The ÎF/F fPLC FRET RV shows a FRET decrease (net 5-phosphatase reaction, solid line, closed symbols) for both Xl-VSP1 and 2 indicating both dephosphorylate PI(4,5)P2. (E) Oocytes were injected with gPLC and either Xl-VSP1, Xl-VSP2 or Xl2-CS. (left) Averaged gPLC fluorescence traces over time during a voltage step from a holding potential of -100 to +160 mV for gPLC co-expressed with either Xl-VSP1 or Xl-VSP2. The kinetics of activation are significantly faster for Xl-VSP1 than for Xl-VSP2. (right) GFP fluorescence measurements were tested at several voltages and the fluorescence voltage relationship plotted showing a fluorescence increase (net 3-phosphatase reaction, dashed line, open symbols). While Xl-VSP1 shows significant levels of 3-phosphatase activity against PI(3,4,5)P3, Xl-VSP2 is a much less efficient 3-phosphatase. All error bars are Â± SEM., n â¥ 9. Data fit with single Boltzmann equations. For each experiment, the oocyte was incubated in 8 Î¼M insulin to increase PI(3,4,5)P3 concentrations.

References [+] :

Balla, Phosphoinositides: tiny lipids with giant impact on cell regulation. 2013, Pubmed