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Insects
2020 Mar 18;113:. doi: 10.3390/insects11030193.
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Functional Characterization of Sex Pheromone Receptors in the Fall Armyworm (Spodoptera frugiperda).
Guo JM
,
Liu XL
,
Liu SR
,
Wei ZQ
,
Han WK
,
Guo Y
,
Dong SL
.
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Pheromone receptors (PRs) found in the antennae of male moths play a vital role in the recognition of sex pheromones released by females. The fall armyworm (FAW), Spodoptera frugiperda, is a notorious invasive pest, but its PRs have not been reported. In this report, six candidate PRs (SfruOR6, 11, 13, 16, 56 and 62) suggested by phylogenetic analysis were cloned, and their tissue-sex expression profiles were determined by quantitative real-time PCR (qPCR). All six genes except for SfruOR6 were highly and specifically expressed in the antennae, with SfruOR6, 13 and 62 being male-specific, while the other three (SfruOR11, 16 and 56) were male biased, suggesting their roles in sex pheromone perception. A functional analysis by the Xenopus oocyte system further demonstrated that SfruOR13 was highly sensitive to the major sex pheromone component Z9-14:OAc and the pheromone analog Z9,E12-14:OAc, but less sensitive to the minor pheromone component Z9-12:OAc; SfruOR16 responded weakly to pheromone component Z9-14:OAc, but strongly to pheromone analog Z9-14:OH; the other four candidate PRs did not respond to any of the four pheromone components and four pheromone analogs. This study contributes to clarifying the pheromone perception in the FAW, and provides potential gene targets for developing OR-based pest control techniques.
Figure 1. Alignment of the amino acid sequences of the six candidate PRs. The approximate positions of the seven predicted transmembrane domains (TM1âTM7) are indicated by black lines.
Figure 2. Phylogenetic analysis of ORs from four Spodoptera species. Sfru: S. frugiperda, Slitu: S. litura, Slitt: S. littoralis and Sexi: S. exigua. The six candidate PR genes in S. frugiperda are all clustered in the PR clade, and are denoted with black stars.
Figure 3. Tissue-sex expression profiles of six candidate SfruPR genes assessed by qPCR. An, antennae; P, proboscises; T, thoraxes; Ab, abdomens; L, legs; W, wings.
Figure 4. Responses of Xenopus oocytes, co-expressing SfruOR13/ORco, to sex pheromone components and analogs. (A) Inward current values of oocytes, injected with SfruOR13/ORco (upper) and with buffer (lower), induced by compounds at concentrations of 10â4 M. (B) Response profile of Xenopus oocytes expressing the SfruOR13/ORco complex. Error bars indicate SEM (n = 6). (C) Responses of Xenopus oocytes, co-expressing SfruOR13/ORco, to Z9-14:OAc at varying concentrations. (D) Dose-response curve of Xenopus oocytes, co-expressing SfruOR13/ORco, to Z9-14:OAc. EC50 value was calculated to be 6.078 Ã 10â6 M. Error bars indicate SEM (n = 5).
Figure 5. Responses of Xenopus oocytes, co-expressing SfruOR16/ORco, to sex pheromone components and analogs. (A) Inward current responses of Xenopus oocytes, injected with SfruOR16/ORco (upper) and with buffer (lower), induced compounds at concentrations of 10â4 M. (B) Response profile of Xenopus oocytes expressing SfruOR16/ORco. Error bars indicate SEM (n = 6). (C) Responses of Xenopus oocytes, co-expressing with SfruOR16/ORco, to Z9-14:OH at varying concentrations. (D) Dose-response curves of Xenopus oocytes, co-expressing SfruOR16/ORco, to Z9-14:OH. EC50 value was calculated to be 1.691 Ã 10â5 M. Error bars indicate SEM (n = 5).
Figure 6. Response profiles of PRs from four Spodoptera species. The left part is the phylogenetic tree of candidate PRs from the four species. The right is the response profiles of these candidate PRs (S. frugiperda (Figure 4 and Figure 5), S. exigua [18], S. littoralis [15,69], and S. litura [19]). The dots represent the responses obtained by the Xenopus oocytes system, and the stars represent responses by in vivo heterologous expression in Drosophila OSNs. The blanks indicate compounds that are not tested. The color of the pheromone component corresponds to the color of the insect species to which the sex pheromone belongs, and the black refers to the components shared by two or more species. Z9-14:OAc is shared by the four Spodoptera species; Z9,E12-14:OAc is shared by S. litura, S. littoralis and S. exigua; Z9,E11-14:OAc and E11-14:OAc are shared by S. litura and S. littoralis. Z11-16:OH and Z9-16:Ald in gray are not sex pheromones for the four Spodoptera species.
Bastin-Héline,
A novel lineage of candidate pheromone receptors for sex communication in moths.
2019, Pubmed,
Xenbase
Bastin-Héline,
A novel lineage of candidate pheromone receptors for sex communication in moths.
2019,
Pubmed
,
Xenbase
Batista-Pereira,
Isolation, identification, synthesis, and field evaluation of the sex pheromone of the Brazilian population of Spodoptera frugiperda.
2006,
Pubmed
Benton,
Variant ionotropic glutamate receptors as chemosensory receptors in Drosophila.
2009,
Pubmed
Buck,
A novel multigene family may encode odorant receptors: a molecular basis for odor recognition.
1991,
Pubmed
Butterwick,
Cryo-EM structure of the insect olfactory receptor Orco.
2018,
Pubmed
Chang,
Pheromone binding proteins enhance the sensitivity of olfactory receptors to sex pheromones in Chilo suppressalis.
2015,
Pubmed
,
Xenbase
Chang,
A Pheromone Antagonist Regulates Optimal Mating Time in the Moth Helicoverpa armigera.
2017,
Pubmed
Cheng,
Genomic adaptation to polyphagy and insecticides in a major East Asian noctuid pest.
2017,
Pubmed
Cock,
Molecular methods to detect Spodoptera frugiperda in Ghana, and implications for monitoring the spread of invasive species in developing countries.
2017,
Pubmed
Darriba,
ProtTest 3: fast selection of best-fit models of protein evolution.
2011,
Pubmed
de Fouchier,
Functional evolution of Lepidoptera olfactory receptors revealed by deorphanization of a moth repertoire.
2017,
Pubmed
Dumas,
Spodoptera frugiperda (Lepidoptera: Noctuidae) host-plant variants: two host strains or two distinct species?
2015,
Pubmed
Fleischer,
Access to the odor world: olfactory receptors and their role for signal transduction in insects.
2018,
Pubmed
Forstner,
A receptor and binding protein interplay in the detection of a distinct pheromone component in the silkmoth Antheraea polyphemus.
2009,
Pubmed
Goergen,
First Report of Outbreaks of the Fall Armyworm Spodoptera frugiperda (J E Smith) (Lepidoptera, Noctuidae), a New Alien Invasive Pest in West and Central Africa.
2016,
Pubmed
Gouin,
Two genomes of highly polyphagous lepidopteran pests (Spodoptera frugiperda, Noctuidae) with different host-plant ranges.
2017,
Pubmed
Groot,
Host strain specific sex pheromone variation in Spodoptera frugiperda.
2008,
Pubmed
Groot,
Experimental evidence for interspecific directional selection on moth pheromone communication.
2006,
Pubmed
Grosse-Wilde,
Candidate pheromone receptors provide the basis for the response of distinct antennal neurons to pheromonal compounds.
2007,
Pubmed
Große-Wilde,
Sex-specific odorant receptors of the tobacco hornworm manduca sexta.
2010,
Pubmed
Hallem,
The molecular basis of odor coding in the Drosophila antenna.
2004,
Pubmed
Hildebrand,
Mechanisms of olfactory discrimination: converging evidence for common principles across phyla.
1997,
Pubmed
Huot,
Spodoptera frugiperda transcriptional response to infestation by Steinernema carpocapsae.
2019,
Pubmed
Ishida,
Chiral discrimination of the Japanese beetle sex pheromone and a behavioral antagonist by a pheromone-degrading enzyme.
2008,
Pubmed
Katoh,
MAFFT multiple sequence alignment software version 7: improvements in performance and usability.
2013,
Pubmed
Kurtovic,
A single class of olfactory neurons mediates behavioural responses to a Drosophila sex pheromone.
2007,
Pubmed
Leal,
Odorant reception in insects: roles of receptors, binding proteins, and degrading enzymes.
2013,
Pubmed
Li,
Requirement for Drosophila SNMP1 for rapid activation and termination of pheromone-induced activity.
2014,
Pubmed
,
Xenbase
Liu,
Identification and functional characterization of sex pheromone receptors in beet armyworm Spodoptera exigua (Hübner).
2013,
Pubmed
,
Xenbase
Liu,
Cloning and functional characterization of three new pheromone receptors from the diamondback moth, Plutella xylostella.
2018,
Pubmed
,
Xenbase
Liu,
Functional characterization of pheromone receptors in the moth Athetis dissimilis (Lepidoptera: Noctuidae).
2019,
Pubmed
,
Xenbase
Liu,
Functional Studies of Sex Pheromone Receptors in Asian Corn Borer Ostrinia furnacalis.
2018,
Pubmed
,
Xenbase
Liu,
Functional specificity of sex pheromone receptors in the cotton bollworm Helicoverpa armigera.
2013,
Pubmed
Livak,
Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method.
2001,
Pubmed
Montagné,
Functional characterization of a sex pheromone receptor in the pest moth Spodoptera littoralis by heterologous expression in Drosophila.
2012,
Pubmed
Pashley,
QUANTITATIVE GENETICS, DEVELOPMENT, AND PHYSIOLOGICAL ADAPTATION IN HOST STRAINS OF FALL ARMYWORM.
1988,
Pubmed
Pregitzer,
The sensory neurone membrane protein SNMP1 contributes to the sensitivity of a pheromone detection system.
2014,
Pubmed
Rogers,
Snmp-1, a novel membrane protein of olfactory neurons of the silk moth Antheraea polyphemus with homology to the CD36 family of membrane proteins.
1997,
Pubmed
Sakurai,
Identification and functional characterization of a sex pheromone receptor in the silkmoth Bombyx mori.
2004,
Pubmed
,
Xenbase
Stamatakis,
RAxML version 8: a tool for phylogenetic analysis and post-analysis of large phylogenies.
2014,
Pubmed
Sun,
Identification and characterization of pheromone receptors and interplay between receptors and pheromone binding proteins in the diamondback moth, Plutella xyllostella.
2013,
Pubmed
,
Xenbase
Tumlinson,
Sex pheromone of fall armyworm,Spodoptera frugiperda (J.E. Smith) : Identification of components critical to attraction in the field.
1986,
Pubmed
Vogt,
Pheromone binding and inactivation by moth antennae.
,
Pubmed
Vosshall,
A spatial map of olfactory receptor expression in the Drosophila antenna.
1999,
Pubmed
Walker,
Transcriptome Analysis of Gene Families Involved in Chemosensory Function in Spodoptera littoralis (Lepidoptera: Noctuidae).
2019,
Pubmed
Wang,
Functional characterization of pheromone receptors in the tobacco budworm Heliothis virescens.
2011,
Pubmed
Yan,
Two Sympatric Spodoptera Species Could Mutually Recognize Sex Pheromone Components for Behavioral Isolation.
2019,
Pubmed
Zhang,
Identification and functional characterization of sex pheromone receptors in the common cutworm (Spodoptera litura).
2015,
Pubmed
,
Xenbase
