XB-ART-57126
Mol Cell
2020 Jul 16;792:221-233.e5. doi: 10.1016/j.molcel.2020.06.014.
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The Histone Chaperone FACT Induces Cas9 Multi-turnover Behavior and Modifies Genome Manipulation in Human Cells.
Wang AS
,
Chen LC
,
Wu RA
,
Hao Y
,
McSwiggen DT
,
Heckert AB
,
Richardson CD
,
Gowen BG
,
Kazane KR
,
Vu JT
,
Wyman SK
,
Shin JJ
,
Darzacq X
,
Walter JC
,
Corn JE
.
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Cas9 is a prokaryotic RNA-guided DNA endonuclease that binds substrates tightly in vitro but turns over rapidly when used to manipulate genomes in eukaryotic cells. Little is known about the factors responsible for dislodging Cas9 or how they influence genome engineering. Unbiased detection through proximity labeling of transient protein interactions in cell-free Xenopus laevis egg extract identified the dimeric histone chaperone facilitates chromatin transcription (FACT) as an interactor of substrate-bound Cas9. FACT is both necessary and sufficient to displace dCas9, and FACT immunodepletion converts Cas9's activity from multi-turnover to single turnover. In human cells, FACT depletion extends dCas9 residence times, delays genome editing, and alters the balance between indel formation and homology-directed repair. FACT knockdown also increases epigenetic marking by dCas9-based transcriptional effectors with a concomitant enhancement of transcriptional modulation. FACT thus shapes the intrinsic cellular response to Cas9-based genome manipulation most likely by determining Cas9 residence times.
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Howard Hughes Medical Institute , UO1-497 EB021236 NIBIB NIH HHS , U54 DK107980 NIDDK NIH HHS , T32 GM098218 NIGMS NIH HHS , R01 HL098316 NHLBI NIH HHS , S10 OD018174 NIH HHS , U01 DA047729 NIDA NIH HHS , U01 EB021236 NIBIB NIH HHS , T32 GM139780 NIGMS NIH HHS
Species referenced: Xenopus laevis
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