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All paired sensory organs arise from a common precursor domain called the pre-placodal region (PPR). In Xenopus, Zic1 non-cell autonomously regulates PPR formation by activating retinoic acid (RA) production. Here, we have identified two Zic1 targets, the RA catabolizing enzyme Cyp26c1 and the transcription factor Pitx2c, expressed in the vicinity of the PPR as being crucially required for maintaining low RA levels in a spatially restricted, PPR-adjacent domain. Morpholino- or CRISPR/Cas9-mediated Cyp26c1 knockdown abrogated PPR gene expression, yielding defective cranial placodes. Direct measurement of RA levels revealed that this is mediated by a mechanism involving excess RA accumulation. Furthermore, we show that pitx2c is activated by RA and required for Cyp26c1 expression in a domain-specific manner through induction of FGF8. We propose that Zic1 anteriorly establishes a program of RA containment and regulation through activation of Cyp26c1 and Pitx2c that cooperates to promote PPR specification in a spatially restricted domain.
Abu-Abed,
The retinoic acid-metabolizing enzyme, CYP26A1, is essential for normal hindbrain patterning, vertebral identity, and development of posterior structures.
2001, Pubmed
Abu-Abed,
The retinoic acid-metabolizing enzyme, CYP26A1, is essential for normal hindbrain patterning, vertebral identity, and development of posterior structures.
2001,
Pubmed
Ahrens,
Tissues and signals involved in the induction of placodal Six1 expression in Xenopus laevis.
2005,
Pubmed
,
Xenbase
Bae,
Identification of Pax3 and Zic1 targets in the developing neural crest.
2014,
Pubmed
,
Xenbase
Baker,
Vertebrate cranial placodes I. Embryonic induction.
2001,
Pubmed
,
Xenbase
Baron,
Retinoic acid and its 4-oxo metabolites are functionally active in human skin cells in vitro.
2005,
Pubmed
Blumberg,
Novel retinoic acid receptor ligands in Xenopus embryos.
1996,
Pubmed
,
Xenbase
Bok,
Transient retinoic acid signaling confers anterior-posterior polarity to the inner ear.
2011,
Pubmed
Chawla,
Retinoic Acid and Pitx2 Regulate Early Neural Crest Survival and Migration in Craniofacial and Ocular Development.
2016,
Pubmed
Christen,
FGF-8 is associated with anteroposterior patterning and limb regeneration in Xenopus.
1997,
Pubmed
,
Xenbase
Cunningham,
Antagonism between retinoic acid and fibroblast growth factor signaling during limb development.
2013,
Pubmed
da Silva,
Fgf8 Expression and Degradation of Retinoic Acid Are Required for Patterning a High-Acuity Area in the Retina.
2017,
Pubmed
Demartis,
Cloning and developmental expression of LFB3/HNF1 beta transcription factor in Xenopus laevis.
1994,
Pubmed
,
Xenbase
Devotta,
Sf3b4-depleted Xenopus embryos: A model to study the pathogenesis of craniofacial defects in Nager syndrome.
2016,
Pubmed
,
Xenbase
Dubey,
Generating retinoic acid gradients by local degradation during craniofacial development: One cell's cue is another cell's poison.
2018,
Pubmed
Duester,
Retinoic acid synthesis and signaling during early organogenesis.
2008,
Pubmed
Durston,
Retinoic acid causes an anteroposterior transformation in the developing central nervous system.
1989,
Pubmed
,
Xenbase
Fletcher,
FGF8 spliceforms mediate early mesoderm and posterior neural tissue formation in Xenopus.
2006,
Pubmed
,
Xenbase
Gere-Becker,
Retinoic acid-induced expression of Hnf1b and Fzd4 is required for pancreas development in Xenopus laevis.
2018,
Pubmed
,
Xenbase
Grocott,
The peripheral sensory nervous system in the vertebrate head: a gene regulatory perspective.
2012,
Pubmed
Harland,
In situ hybridization: an improved whole-mount method for Xenopus embryos.
1991,
Pubmed
,
Xenbase
Hensey,
Programmed cell death during Xenopus development: a spatio-temporal analysis.
1998,
Pubmed
,
Xenbase
Hernandez,
vhnf1 integrates global RA patterning and local FGF signals to direct posterior hindbrain development in zebrafish.
2004,
Pubmed
Hong,
Fgf8a induces neural crest indirectly through the activation of Wnt8 in the paraxial mesoderm.
2008,
Pubmed
,
Xenbase
Hong,
The activity of Pax3 and Zic1 regulates three distinct cell fates at the neural plate border.
2007,
Pubmed
,
Xenbase
Huang,
The doublesex-related gene, XDmrt4, is required for neurogenesis in the olfactory system.
2005,
Pubmed
,
Xenbase
Janesick,
RIPPLY3 is a retinoic acid-inducible repressor required for setting the borders of the pre-placodal ectoderm.
2012,
Pubmed
,
Xenbase
Jaurena,
Zic1 controls placode progenitor formation non-cell autonomously by regulating retinoic acid production and transport.
2015,
Pubmed
,
Xenbase
Jeong,
Developmental expression of Pitx2c in Xenopus trigeminal and profundal placodes.
2014,
Pubmed
,
Xenbase
Jones,
Use of fast HPLC multiple reaction monitoring cubed for endogenous retinoic acid quantification in complex matrices.
2015,
Pubmed
Kane,
HPLC/UV quantitation of retinal, retinol, and retinyl esters in serum and tissues.
2008,
Pubmed
Kane,
Quantitative profiling of endogenous retinoic acid in vivo and in vitro by tandem mass spectrometry.
2008,
Pubmed
Kane,
Quantification of endogenous retinoids.
2010,
Pubmed
Kane,
Quantification of endogenous retinoic acid in limited biological samples by LC/MS/MS.
2005,
Pubmed
Kitson,
The effect of disulfiram on the aldehyde dehydrogenases of sheep liver.
1975,
Pubmed
Kolm,
Efficient hormone-inducible protein function in Xenopus laevis.
1995,
Pubmed
,
Xenbase
Kudoh,
Distinct roles for Fgf, Wnt and retinoic acid in posteriorizing the neural ectoderm.
2002,
Pubmed
Kumar,
Retinoic acid signaling in perioptic mesenchyme represses Wnt signaling via induction of Pitx2 and Dkk2.
2010,
Pubmed
Lea,
Temporal and spatial expression of FGF ligands and receptors during Xenopus development.
2009,
Pubmed
,
Xenbase
Le Douarin,
Cell line segregation during peripheral nervous system ontogeny.
1986,
Pubmed
Li,
EYA1 mutations associated with the branchio-oto-renal syndrome result in defective otic development in Xenopus laevis.
2010,
Pubmed
,
Xenbase
Liu,
Regulation of left-right asymmetry by thresholds of Pitx2c activity.
2001,
Pubmed
Liu,
Genetic dissection of Pitx2 in craniofacial development uncovers new functions in branchial arch morphogenesis, late aspects of tooth morphogenesis and cell migration.
2003,
Pubmed
Lynch,
Analysis of the expression of retinoic acid metabolising genes during Xenopus laevis organogenesis.
2011,
Pubmed
,
Xenbase
Matt,
Retinoic acid-dependent eye morphogenesis is orchestrated by neural crest cells.
2005,
Pubmed
Mayor,
Induction of the prospective neural crest of Xenopus.
1995,
Pubmed
,
Xenbase
Mizuseki,
Xenopus Zic-related-1 and Sox-2, two factors induced by chordin, have distinct activities in the initiation of neural induction.
1998,
Pubmed
,
Xenbase
Mohammadi,
Structures of the tyrosine kinase domain of fibroblast growth factor receptor in complex with inhibitors.
1997,
Pubmed
Moody,
Transcriptional regulation of cranial sensory placode development.
2015,
Pubmed
Moody,
Fates of the blastomeres of the 16-cell stage Xenopus embryo.
1987,
Pubmed
,
Xenbase
Moreno,
Regulation of segmental patterning by retinoic acid signaling during Xenopus somitogenesis.
2004,
Pubmed
,
Xenbase
Nakayama,
Simple and efficient CRISPR/Cas9-mediated targeted mutagenesis in Xenopus tropicalis.
2013,
Pubmed
,
Xenbase
Nakayama,
Cas9-based genome editing in Xenopus tropicalis.
2014,
Pubmed
,
Xenbase
Ono,
Retinoic acid degradation shapes zonal development of vestibular organs and sensitivity to transient linear accelerations.
2020,
Pubmed
Pandur,
Xenopus Six1 gene is expressed in neurogenic cranial placodes and maintained in the differentiating lateral lines.
2000,
Pubmed
,
Xenbase
Paschaki,
Retinoic acid regulates olfactory progenitor cell fate and differentiation.
2013,
Pubmed
Pijnappel,
The retinoid ligand 4-oxo-retinoic acid is a highly active modulator of positional specification.
1993,
Pubmed
,
Xenbase
Pohl,
Sequence and expression of FoxB2 (XFD-5) and FoxI1c (XFD-10) in Xenopus embryogenesis.
2002,
Pubmed
,
Xenbase
Ruf,
SIX1 mutations cause branchio-oto-renal syndrome by disruption of EYA1-SIX1-DNA complexes.
2004,
Pubmed
Saint-Jeannet,
Whole-Mount In Situ Hybridization of Xenopus Embryos.
2017,
Pubmed
,
Xenbase
Saint-Jeannet,
Establishing the pre-placodal region and breaking it into placodes with distinct identities.
2014,
Pubmed
,
Xenbase
Saka,
Spatial and temporal patterns of cell division during early Xenopus embryogenesis.
2001,
Pubmed
,
Xenbase
Sato,
Neural crest determination by co-activation of Pax3 and Zic1 genes in Xenopus ectoderm.
2005,
Pubmed
,
Xenbase
Schlosser,
Induction and specification of cranial placodes.
2006,
Pubmed
,
Xenbase
Schlosser,
Molecular anatomy of placode development in Xenopus laevis.
2004,
Pubmed
,
Xenbase
Schönberger,
Mutation in the transcriptional coactivator EYA4 causes dilated cardiomyopathy and sensorineural hearing loss.
2005,
Pubmed
Shiotsugu,
Multiple points of interaction between retinoic acid and FGF signaling during embryonic axis formation.
2004,
Pubmed
,
Xenbase
Sive,
Identification of a retinoic acid-sensitive period during primary axis formation in Xenopus laevis.
1990,
Pubmed
,
Xenbase
Slack,
An interaction between dorsal and ventral regions of the marginal zone in early amphibian embryos.
1980,
Pubmed
,
Xenbase
Takabatake,
Conserved and divergent expression of T-box genes Tbx2-Tbx5 in Xenopus.
2000,
Pubmed
,
Xenbase
Tanibe,
Retinoic acid metabolizing factor xCyp26c is specifically expressed in neuroectoderm and regulates anterior neural patterning in Xenopus laevis.
2008,
Pubmed
,
Xenbase
Uehara,
CYP26A1 and CYP26C1 cooperatively regulate anterior-posterior patterning of the developing brain and the production of migratory cranial neural crest cells in the mouse.
2007,
Pubmed
Veverka,
Inhibition of aldehyde dehydrogenase by disulfiram and its metabolite methyl diethylthiocarbamoyl-sulfoxide.
1997,
Pubmed
Villanueva,
Posteriorization by FGF, Wnt, and retinoic acid is required for neural crest induction.
2002,
Pubmed
,
Xenbase
White,
Complex regulation of cyp26a1 creates a robust retinoic acid gradient in the zebrafish embryo.
2007,
Pubmed
Xu,
Eya1 is required for the morphogenesis of mammalian thymus, parathyroid and thyroid.
2002,
Pubmed
Yang,
Analysis of FGF-dependent and FGF-independent pathways in otic placode induction.
2013,
Pubmed
Yu,
xCyp26c Induced by Inhibition of BMP Signaling Is Involved in Anterior-Posterior Neural Patterning of Xenopus laevis.
2016,
Pubmed
,
Xenbase
Zhong,
CYP26C1 Is a Hydroxylase of Multiple Active Retinoids and Interacts with Cellular Retinoic Acid Binding Proteins.
2018,
Pubmed