J Chem Neuroanat 2003 Jan 01;251:53-71. doi: 10.1016/s0891-0618(02)00097-2.

Comparative analysis of neuropeptide FF-like immunoreactivity in the brain of anuran (Rana perezi, Xenopus laevis) and urodele (Pleurodeles waltl) amphibians.

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The neuropeptide FF (NPFF) is a member of the RFamide related peptides (FaRPs) that share the dipeptide Arg-Phe-NH2 at their C-terminal. It was originally isolated from bovine brain and its wide distribution has been demonstrated in the brain of several mammalian species. By means of an NPFF antiserum we have investigated the distribution pattern of NPFF-like immunoreactive cells and fibers in the brain of anuran and urodele amphibians. In both amphibian orders, the most conspicuous labeled cell population was found in the preoptic area and hypothalamus, primarily in the suprachiasmatic region. Numerous fibers reached the median eminence and the intermediate lobe of the hypophysis. Only in the anuran brain cells were observed in the pallium and septum. In the urodele, cells and fibers of the terminal nerve were distinctly labeled. Cell bodies were widely distributed in the reticular formation of anurans and, in both orders, a large cell population was found in the nucleus of the solitary tract and the spinal cord. Comparable fiber distribution between both orders exists in which the basal telencephalon (mainly the amygdaloid complex), the hypothalamus and the spinal cord are the regions most richly innervated. The distribution pattern of NPFF-like immunorective elements in the brain of amphibians, which only partly overlaps with those of other FaRPs, supports the notion that a NPFF-like peptide exists in amphibians. On the basis of its localization, this peptide may act as a hypophysiotropic neurohormone and be involved in background adaptation. Its wide distribution in similar zones of the brain in amphibians and mammals suggests that functional roles of this peptide have been conserved in vertebrate evolution.

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Species referenced: Xenopus laevis
Genes referenced: npff