Reis AH and Sokol SY (2021), Rspo2 inhibits TCF3 phosphorylation to antagoni...

XB-ART-58233

Sci Rep 2021 Jun 28;111:13433. doi: 10.1038/s41598-021-92824-6.

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Rspo2 inhibits TCF3 phosphorylation to antagonize Wnt signaling during vertebrate anteroposterior axis specification.

Reis AH , Sokol SY .

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The Wnt pathway activates target genes by controlling the β-catenin-T-cell factor (TCF) transcriptional complex during embryonic development and cancer. This pathway can be potentiated by R-spondins, a family of proteins that bind RNF43/ZNRF3 E3 ubiquitin ligases and LGR4/5 receptors to prevent Frizzled degradation. Here we demonstrate that, during Xenopus anteroposterior axis specification, Rspo2 functions as a Wnt antagonist, both morphologically and at the level of gene targets and pathway mediators. Unexpectedly, the binding to RNF43/ZNRF3 and LGR4/5 was not required for the Wnt inhibitory activity. Moreover, Rspo2 did not influence Dishevelled phosphorylation in response to Wnt ligands, suggesting that Frizzled activity is not affected. Further analysis indicated that the Wnt antagonism is due to the inhibitory effect of Rspo2 on TCF3/TCF7L1 phosphorylation that normally leads to target gene activation. Consistent with this mechanism, Rspo2 anteriorizing activity has been rescued in TCF3-depleted embryos. These observations suggest that Rspo2 is a context-specific regulator of TCF3 phosphorylation and Wnt signaling.

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Species referenced: Xenopus laevis
Genes referenced: abcb6 ag1 cdx4 dvl2 eef1a1 foxg1 furin fzd1 krt12.4 krt12.5 lgr4 lgr5 msgn1 myod1 otx2 rnf43 rspo2 tcf3 tcf7 tcf7l1 wnt3a wnt5a wnt8a znrf3
GO keywords: Wnt signaling pathway involved in animal/vegetal axis specification
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	Figure 1. Rspo2 function is essential for anterior development. (A,B) Four-cell embryos were injected with 0.5Â ng of Rspo2 RNA into both animal-ventral blastomeres and cultured until stage 28. (A) Uninjected control embryo. (B) Representative embryo injected with Rspo2 RNA. The penetrance is indicated as the ratio of the number of embryos with the phenotype and the total number of injected embryos. (C) The effect of Rspo2 on gene marker expression. Animal pole explants were dissected at stage 9 from embryos overexpressing Rspo2 RNA or uninjected controls. RT-qPCR analysis was carried out for otx2, ag1, and krt12.4 at stage 18. (D) Altered gene expression in Rspo2 morphants. RNA was isolated from stage 18 control embryos or embryos depleted of Rspo2. RT-qPCR for ag1 and otx2 was carried out in triplicates. (C,D) Each graph is a single experiment with triplicate samples, representative from at least 3 independent experiments. Meansâ€‰+â€‰/âˆ’ s. d. are shown. Statistical significance has been assessed by Studentâ€™s t test, *, pâ€‰<â€‰0.05. (Eâ€“J) In situ hybridization of control and manipulated stage 16 or 25 embryos with krt12.4 (Eâ€“G), foxg1 and cdx4 (Hâ€“J) probes. (Eâ€“G) Width of the anterior neural plate is shown as lack of krt12.4 (arrows). (Hâ€“J) The foxg1 domain is indicated by white arrows, the anterior region lacking cdx4â€”by dashed lines. See Supplementary Table 1 for quantification.
	Figure 2. Rspo2 antagonizes Wnt signaling. (A) Scheme of the experiment. Four-cell embryos were injected animally into both dorsal blastomeres with the indicated constructs and cultured to stage 38. (B) Uninjected control embryo. (C) Headless embryo injected with Wnt8 DNA (50Â pg). (D) Embryo injected with Rspo2 RNA (0.5Â ng). (E) Embryo coexpressing Wnt8 DNA and Rspo2 mRNA. (F) Quantification of the data in (Aâ€“D) representative of 3 independent experiments. Numbers of embryos per group are shown above each bar. ***, pâ€‰<â€‰0.0001, Fisher's exact test. (G) Target gene expression in Wnt8 and Rspo2-stimulated ectoderm explants. Embryos were injected into four animal blastomeres with Wnt8 DNA (50Â pg) and Rspo2 RNA (0.5Â ng), as indicated, and ectoderm explants were prepared at stage 9 and cultured until stage 13. (H) Dorsal marginal zones (DMZ) were dissected at stage 10 from the control and Rspo2 RNA- or RMOATG-injected embryos and cultured until stage 12. (G,H) RT-qPCR analysis was carried out in triplicates for axin2 and cdx4, and normalized to eef1a1 levels. Meansâ€‰+â€‰/âˆ’ s.d. are shown. Graphs are representative of three independent experiments. Statistical significance has been assessed by Studentâ€™s t test, , pâ€‰<â€‰0.05; **, pâ€‰<â€‰0.01.
	Figure 3. The effects of Rspo2 manipulation on Wnt reporter activity in transgenic embryos. (A) Experimental scheme. Xla.Tg(WntREs:dEGFP)Vlemx embryos were injected into one dorsal blastomere with mRFP RNA (50Â pg) with (C) or without (B) Rspo2 RNA (0.5Â ng). GFP fluorescence of the injected embryos at stage 18 is shown. Embryo images are representative of 3 different experiments. Asterisk indicates the injected side of the embryo, brackets in C show the comparison of the injected and the control sides. (D,E) Rspo2 modulates Wnt reporter activation. (D) Rspo2 RNA (0.5Â ng), RMOATG (10Â ng) or RMOSB (20Â ng) were injected at two dorsal blastomeres at 4-cell stage. The embryos were lysed at stage 20 and immunoblotted with anti-GFP antibodies. (E) Four-cell stage embryos were injected animally with Wnt3a RNA (50Â pg) and Rspo2 RNAs (0.5Â ng) or RMOATG (10Â ng). Ectoderm explants were dissected at stage 9 and cultured until stage 13, then lysed and immunoblotted with anti-GFP antibodies. Erk1 is a control for loading. In (E) two right lanes were run in the same gel but away from the left lanes (see Supplementary Fig.Â 5). Five embryos or 10 explants were pooled for each experimental condition in (D,E).
	Figure 4. Rspo2 inhibits TCF3 phosphorylation. (A) Schematic of Rspo2 deletion constructs. SP, signal peptide; FU1, furin-like domain 1; FU2, furin-like domain 2; TSP, thrombospondin type 1 domain; BR, the basic amino acid-rich domain. (B,C) Effects of Rspo2 constructs on Wnt-dependent Dvl2 phosphorylation (B) and TCF3 phosphorylation and Î²-catenin levels (C). Four-cell stage embryos were injected animally with Wnt8 DNA (50Â pg or 100Â pg) or Wnt8, Wnt3a or Wnt5a RNAs (1Â ng each) and Rspo2, Rspoâˆ†F or Rspoâˆ†T RNAs (0.5Â ng each) as indicated. Ectoderm explants were dissected at stage 9 and cultured until stage 12 for immunoblotting with antibodies against Dvl2, TCF3, ABC (non-phosphorylated Î²-catenin). Arrowheads indicate the position of phosphorylated (upshifted) and non-phosphorylated Dvl2 or TCF3 proteins. Erk1 controls for loading. (D) Effects of Rspo2 constructs (0.5Â ng each) on TCF3 phosphorylated by endogenous signals. Dorsal marginal zone (D) and ventral marginal zone (V) were dissected from the control and injected embryos at stage 10 and cultured until stage 12.5 for immunoblotting with anti-TCF3 antibodies as shown. Control D and V groups were run in the same gel but separately from the other groups (see Supplementary Fig.Â 5). (E) Effects of Rspo2 depletion on TCF3 phosphorylation by endogenous signals. DMZ and VMZ explants of embryos injected with control MO (COMO, 20Â ng) or RMOATG (20Â ng) were dissected and analyzed by immunoblotting as in (B,C).
	Figure 5. TCF3 is essential for Rspo2 inhibitory effects. (A) TCF3MO rescues the anteriorized phenotype of Rspoâˆ†T RNA overexpressing embryos. Four-cell stage embryos were dorsally injected with TCF3MO (30Â ng) and/or Rspoâˆ†T RNA (0.5Â ng). Arrowheads indicate the cement gland. (B) Quantification of the data in (A) representative of two independent experiments. Numbers of embryos per group are shown above each bar. (C) Rspoâˆ†T expression levels are not altered by TCF3MO in ectoderm explants (stage 12) in two independent experiments (Exp 1 and Exp 2). âˆ†T, Rspoâˆ†T; TMO, TCF3MO.
	Figure 6. Rspo2 inhibits Wnt signaling through TCF3. (A) âˆ†Î²TCF3 RNA (10Â pg) rescues ag1, otx2, cdx4, and msgn1 expression in embryos injected with 10Â ng of RMOATG. B, C, Rspo2 inhibits axin2 upregulation by Wnt8 (C) but not TCF1 (B) in ectoderm cells. Embryos were injected with Wnt8 (20Â pg) or TCF1 (100Â pg) RNA without or with Rspo2 RNA (300Â pg). Ectoderm explants were prepared at stage 8.5â€“9 and analyzed at stage 13. RT-qPCR analysis was carried out in triplicates for axin2 and normalized to eef1a1 levels. Meansâ€‰+â€‰/âˆ’ s.d. are shown. Graphs are representative of 2â€“4 independent experiments. Statistical significance has been assessed by Studentâ€™s t test, *, pâ€‰<â€‰0.05. D, Model for Rspo2-mediated repression. Rspo2 functions via an unknown receptor to inhibit Wnt target gene activation mediated by TCF3 phosphorylation but not TCF1-dependent transcriptional responses.

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