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BACKGROUND: hes genes are chordate homologs of Drosophila genes, hairy and enhancer of split, which encode a basic helix-loop-helix (bHLH) transcriptional repressor with a WRPW motif. Various developmental functions of hes genes, including early embryogenesis and neurogenesis, have been elucidated in vertebrates. However, their orthologous relationships remain unclear partly because of less conservation of relatively short amino acid sequences, the fact that the genome was not analyzed as it is today, and species-specific genome duplication. This results in complicated gene names in vertebrates, which are not consistent in orthologs. We previously revealed that Xenopus frogs have two clusters of hes5, named "the hes5.1 cluster" and "the hes5.3 cluster", but the origin and the conservation have not yet been revealed.
RESULTS: Here, we elucidated the orthologous and paralogous relationships of all hes genes of human, mouse, chicken, gecko, zebrafish, medaka, coelacanth, spotted gar, elephant shark and three species of frogs, Xenopus tropicalis (X. tropicalis), X. laevis, Nanorana parkeri, by phylogenetic and synteny analyses. Any duplicated hes5 were not found in mammals, whereas hes5 clusters in teleost were conserved although not as many genes as the three frog species. In addition, hes5 cluster-like structure was found in the elephant shark genome, but not found in cyclostomata.
CONCLUSION: These data suggest that the hes5 cluster existed in the gnathostome ancestor but became a single gene in mammals. The number of hes5 cluster genes were specifically large in frogs.
Bisbee,
Albumin phylogeny for clawed frogs (Xenopus).
1977, Pubmed,
Xenbase
Bisbee,
Albumin phylogeny for clawed frogs (Xenopus).
1977,
Pubmed
,
Xenbase
Capella-Gutiérrez,
trimAl: a tool for automated alignment trimming in large-scale phylogenetic analyses.
2009,
Pubmed
Davis,
Vertebrate hairy and Enhancer of split related proteins: transcriptional repressors regulating cellular differentiation and embryonic patterning.
2001,
Pubmed
,
Xenbase
Edgar,
MUSCLE: multiple sequence alignment with high accuracy and high throughput.
2004,
Pubmed
Fisher,
The WRPW motif of the hairy-related basic helix-loop-helix repressor proteins acts as a 4-amino-acid transcription repression and protein-protein interaction domain.
1996,
Pubmed
Harland,
Xenopus research: metamorphosed by genetics and genomics.
2011,
Pubmed
,
Xenbase
Hatakeyama,
Hes genes regulate size, shape and histogenesis of the nervous system by control of the timing of neural stem cell differentiation.
2004,
Pubmed
Kageyama,
The Hes gene family: repressors and oscillators that orchestrate embryogenesis.
2007,
Pubmed
Katoh,
Identification and characterization of human HES2, HES3, and HES5 genes in silico.
2004,
Pubmed
Katoh,
MAFFT multiple sequence alignment software version 7: improvements in performance and usability.
2013,
Pubmed
Kellis,
Proof and evolutionary analysis of ancient genome duplication in the yeast Saccharomyces cerevisiae.
2004,
Pubmed
Lawrence,
Selfish operons: horizontal transfer may drive the evolution of gene clusters.
1996,
Pubmed
Lawrence,
Selfish operons: the evolutionary impact of gene clustering in prokaryotes and eukaryotes.
1999,
Pubmed
Minguillón,
The amphioxus Hairy family: differential fate after duplication.
2003,
Pubmed
Pourquié,
Vertebrate somitogenesis: a novel paradigm for animal segmentation?
2003,
Pubmed
Session,
Genome evolution in the allotetraploid frog Xenopus laevis.
2016,
Pubmed
,
Xenbase
Shinga,
Early patterning of the prospective midbrain-hindbrain boundary by the HES-related gene XHR1 in Xenopus embryos.
2001,
Pubmed
,
Xenbase
Stamatakis,
RAxML version 8: a tool for phylogenetic analysis and post-analysis of large phylogenies.
2014,
Pubmed
Takada,
Identification of target genes for the Xenopus Hes-related protein XHR1, a prepattern factor specifying the midbrain-hindbrain boundary.
2005,
Pubmed
,
Xenbase
Tanigaki,
Notch1 and Notch3 instructively restrict bFGF-responsive multipotent neural progenitor cells to an astroglial fate.
2001,
Pubmed
Van de Peer,
The evolutionary significance of ancient genome duplications.
2009,
Pubmed
Watanabe,
Conservatism and variability of gene expression profiles among homeologous transcription factors in Xenopus laevis.
2017,
Pubmed
,
Xenbase
Zhou,
Comparative and evolutionary analysis of the HES/HEY gene family reveal exon/intron loss and teleost specific duplication events.
2012,
Pubmed
