Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
???displayArticle.abstract???
Ca2+ entry through Cav1.3 Ca2+ channels plays essential roles in diverse physiological events. We employed yeast-two-hybrid (Y2H) assays to mine novel proteins interacting with Cav1.3 and found Snapin2, a synaptic protein, as a partner interacting with the long carboxyl terminus (CTL) of rat Cav1.3L variant. Co-expression of Snapin with Cav1.3L/Cavβ3/α2δ2 subunits increased the peak current density or amplitude by about 2-fold in HEK-293 cells and Xenopus oocytes, without affecting voltage-dependent gating properties and calcium-dependent inactivation. However, the Snapin up-regulation effect was not found for rat Cav1.3S containing a short CT (CTS) in which a Snapin interaction site in the CTL was deficient. Luminometry and electrophysiology studies uncovered that Snapin co-expression did not alter the membrane expression of HA tagged Cav1.3L but increased the slope of tail current amplitudes plotted against ON-gating currents, indicating that Snapin increases the opening probability of Cav1.3L. Taken together, our results strongly suggest that Snapin directly interacts with the CTL of Cav1.3L, leading to up-regulation of Cav1.3L channel activity via facilitating channel opening probability.
Azizan,
Somatic mutations in ATP1A1 and CACNA1D underlie a common subtype of adrenal hypertension.
2013, Pubmed
Azizan,
Somatic mutations in ATP1A1 and CACNA1D underlie a common subtype of adrenal hypertension.
2013,
Pubmed
Banerjee,
Bilobal architecture is a requirement for calmodulin signaling to CaV1.3 channels.
2018,
Pubmed
Bech-Hansen,
Loss-of-function mutations in a calcium-channel alpha1-subunit gene in Xp11.23 cause incomplete X-linked congenital stationary night blindness.
1998,
Pubmed
Bock,
Functional properties of a newly identified C-terminal splice variant of Cav1.3 L-type Ca2+ channels.
2011,
Pubmed
Buxton,
Identification and characterization of Snapin as a ubiquitously expressed SNARE-binding protein that interacts with SNAP23 in non-neuronal cells.
2003,
Pubmed
Cai,
Snapin-regulated late endosomal transport is critical for efficient autophagy-lysosomal function in neurons.
2010,
Pubmed
Chheda,
Phosphorylation of Snapin by PKA modulates its interaction with the SNARE complex.
2001,
Pubmed
Dickman,
Snapin is critical for presynaptic homeostatic plasticity.
2012,
Pubmed
Di Giovanni,
Regulation of synaptic activity by snapin-mediated endolysosomal transport and sorting.
2015,
Pubmed
Dolphin,
Presynaptic calcium channels: specialized control of synaptic neurotransmitter release.
2020,
Pubmed
Goswami,
TRPV1 acts as a synaptic protein and regulates vesicle recycling.
2010,
Pubmed
Ilardi,
Snapin: a SNARE-associated protein implicated in synaptic transmission.
1999,
Pubmed
Kang,
Molecular cloning and characterization of a hamster Cav1.3 Ca2+ channel variant with a long carboxyl terminus.
2011,
Pubmed
,
Xenbase
Kim,
Reduction of Cav1.3 channels in dorsal hippocampus impairs the development of dentate gyrus newborn neurons and hippocampal-dependent memory tasks.
2017,
Pubmed
Lee,
Assembly and architecture of biogenesis of lysosome-related organelles complex-1 (BLOC-1).
2012,
Pubmed
Ma,
Exploring the dominant role of Cav1 channels in signalling to the nucleus.
2012,
Pubmed
Mangoni,
Functional role of L-type Cav1.3 Ca2+ channels in cardiac pacemaker activity.
2003,
Pubmed
Marschallinger,
The L-type calcium channel Cav1.3 is required for proper hippocampal neurogenesis and cognitive functions.
2015,
Pubmed
McKinney,
Impaired long-term potentiation and enhanced neuronal excitability in the amygdala of Ca(V)1.3 knockout mice.
2009,
Pubmed
Mistry,
Syntaxin specificity of aquaporins in the inner medullary collecting duct.
2009,
Pubmed
,
Xenbase
Namkung,
Requirement for the L-type Ca(2+) channel alpha(1D) subunit in postnatal pancreatic beta cell generation.
2001,
Pubmed
Pan,
Snapin facilitates the synchronization of synaptic vesicle fusion.
2009,
Pubmed
Pinggera,
New gain-of-function mutation shows CACNA1D as recurrently mutated gene in autism spectrum disorders and epilepsy.
2017,
Pubmed
Pinggera,
Cav 1.3 (CACNA1D) L-type Ca2+ channel dysfunction in CNS disorders.
2016,
Pubmed
Platzer,
Congenital deafness and sinoatrial node dysfunction in mice lacking class D L-type Ca2+ channels.
2000,
Pubmed
Scharinger,
Cell-type-specific tuning of Cav1.3 Ca(2+)-channels by a C-terminal automodulatory domain.
2015,
Pubmed
Schmidt,
Snapin accelerates exocytosis at low intracellular calcium concentration in mouse chromaffin cells.
2013,
Pubmed
Shi,
The Contribution of L-Type Cav1.3 Channels to Retinal Light Responses.
2017,
Pubmed
Singh,
Modulation of voltage- and Ca2+-dependent gating of CaV1.3 L-type calcium channels by alternative splicing of a C-terminal regulatory domain.
2008,
Pubmed
Song,
Snapin mediates incretin action and augments glucose-dependent insulin secretion.
2011,
Pubmed
Stanika,
Regulation of Postsynaptic Stability by the L-type Calcium Channel CaV1.3 and its Interaction with PDZ Proteins.
2015,
Pubmed
Sun,
Physical and functional interaction of Snapin with Cav1.3 calcium channel impacts channel protein trafficking in atrial myocytes.
2017,
Pubmed
Tian,
The role of Snapin in neurosecretion: snapin knock-out mice exhibit impaired calcium-dependent exocytosis of large dense-core vesicles in chromaffin cells.
2005,
Pubmed
Toyoda,
CaV1.3 L-type Ca2+ channel contributes to the heartbeat by generating a dihydropyridine-sensitive persistent Na+ current.
2017,
Pubmed
Vandael,
Cav1.3 Channels as Key Regulators of Neuron-Like Firings and Catecholamine Release in Chromaffin Cells.
2015,
Pubmed
Vites,
Reinvestigation of the role of snapin in neurotransmitter release.
2004,
Pubmed
Vivas,
Proximal clustering between BK and CaV1.3 channels promotes functional coupling and BK channel activation at low voltage.
2017,
Pubmed
Xia,
Functional expression of L- and T-type Ca2+ channels in murine HL-1 cells.
2004,
Pubmed
Xu,
Neuronal Ca(V)1.3alpha(1) L-type channels activate at relatively hyperpolarized membrane potentials and are incompletely inhibited by dihydropyridines.
2001,
Pubmed
,
Xenbase
Yu,
Differential roles for snapin and synaptotagmin in the synaptic vesicle cycle.
2013,
Pubmed
Zhang,
Ca1.2 and CaV1.3 neuronal L-type calcium channels: differential targeting and signaling to pCREB.
2006,
Pubmed
,
Xenbase
Zissimopoulos,
Ryanodine receptor interaction with the SNARE-associated protein snapin.
2006,
Pubmed
