Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Identification of a gustatory receptor tuned to sinigrin in the cabbage butterfly Pieris rapae.
Yang J
,
Guo H
,
Jiang NJ
,
Tang R
,
Li GC
,
Huang LQ
,
van Loon JJA
.
???displayArticle.abstract???
Glucosinolates are token stimuli in host selection of many crucifer specialist insects, but the underlying molecular basis for host selection in these insects remains enigmatic. Using a combination of behavioral, electrophysiological, and molecular methods, we investigate glucosinolate receptors in the cabbage butterfly Pieris rapae. Sinigrin, as a potent feeding stimulant, elicited activity in larval maxillary lateral sensilla styloconica, as well as in adult medial tarsal sensilla. Two P. rapae gustatory receptor genes PrapGr28 and PrapGr15 were identified with high expression in female tarsi, and the subsequent functional analyses showed that Xenopus oocytes only expressing PrapGr28 had specific responses to sinigrin; when ectopically expressed in Drosophila sugar sensing neurons, PrapGr28 conferred sinigrin sensitivity to these neurons. RNA interference experiments further showed that knockdown of PrapGr28 reduced the sensitivity of adult medial tarsal sensilla to sinigrin. Taken together, we conclude that PrapGr28 is a gustatory receptor tuned to sinigrin in P. rapae, which paves the way for revealing the molecular basis of the relationships between crucifer plants and their specialist insects.
Agnihotri,
Gustatory receptors in Lepidoptera: chemosensation and beyond.
2016, Pubmed
Agnihotri,
Gustatory receptors in Lepidoptera: chemosensation and beyond.
2016,
Pubmed
Agrawal,
A role for isothiocyanates in plant resistance against the specialist herbivore Pieris rapae.
2003,
Pubmed
Briscoe,
Female behaviour drives expression and evolution of gustatory receptors in butterflies.
2013,
Pubmed
Capella-Gutiérrez,
trimAl: a tool for automated alignment trimming in large-scale phylogenetic analyses.
2009,
Pubmed
Cheng,
Genomic adaptation to polyphagy and insecticides in a major East Asian noctuid pest.
2017,
Pubmed
Clyne,
Candidate taste receptors in Drosophila.
2000,
Pubmed
Delventhal,
Bitter taste receptors confer diverse functions to neurons.
2016,
Pubmed
Dweck,
Molecular Logic and Evolution of Bitter Taste in Drosophila.
2020,
Pubmed
Everaert,
Benchmarking of RNA-sequencing analysis workflows using whole-transcriptome RT-qPCR expression data.
2017,
Pubmed
FRAENKEL,
The raison d'ĕtre of secondary plant substances; these odd chemicals arose as a means of protecting plants from insects and now guide insects to food.
1959,
Pubmed
Fujii,
Drosophila sugar receptors in sweet taste perception, olfaction, and internal nutrient sensing.
2015,
Pubmed
Gols,
Seasonal and herbivore-induced dynamics of foliar glucosinolates in wild cabbage (Brassica oleracea).
2018,
Pubmed
Grabherr,
Full-length transcriptome assembly from RNA-Seq data without a reference genome.
2011,
Pubmed
Harris,
Representations of Taste Modality in the Drosophila Brain.
2015,
Pubmed
Hiroi,
Differentiated response to sugars among labellar chemosensilla in Drosophila.
2002,
Pubmed
Hopkins,
Role of glucosinolates in insect-plant relationships and multitrophic interactions.
2009,
Pubmed
Hou,
Functional characterization of odorant receptors from the moth Eriocrania semipurpurella: A comparison of results in the Xenopus oocyte and HEK cell systems.
2020,
Pubmed
,
Xenbase
Huang,
Oviposition stimulants and deterrents regulating differential acceptance ofIberis amara byPieris rapae andP. napi oleracea.
1993,
Pubmed
Huang,
A chemical basis for differential acceptance ofErysimum cheiranthoides by twoPieris species.
1993,
Pubmed
Huang,
Relative activities of glucosinolates as oviposition stimulants forPieris rapae andP. napi oleracea.
1994,
Pubmed
Kasubuchi,
Insect taste receptors relevant to host identification by recognition of secondary metabolite patterns of non-host plants.
2018,
Pubmed
Koenig,
A reference gene set for chemosensory receptor genes of Manduca sexta.
2015,
Pubmed
Kushad,
Variation of glucosinolates in vegetable crops of Brassica oleracea.
1999,
Pubmed
Kvello,
Central gustatory neurons integrate taste quality information from four appendages in the moth Heliothis virescens.
2010,
Pubmed
Lee,
Avoiding DEET through insect gustatory receptors.
2010,
Pubmed
Li,
RSEM: accurate transcript quantification from RNA-Seq data with or without a reference genome.
2011,
Pubmed
Ling,
The molecular and cellular basis of taste coding in the legs of Drosophila.
2014,
Pubmed
Liu,
Gene editing reveals obligate and modulatory components of the CO2 receptor complex in the malaria vector mosquito, Anopheles coluzzii.
2020,
Pubmed
Livak,
Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method.
2001,
Pubmed
Meyerhof,
The molecular receptive ranges of human TAS2R bitter taste receptors.
2010,
Pubmed
Miles,
Behavioral and chemosensory responses to a host recognition cue by larvae of Pieris rapae.
2005,
Pubmed
Moon,
A taste receptor required for the caffeine response in vivo.
2006,
Pubmed
Moon,
A Drosophila gustatory receptor essential for aversive taste and inhibiting male-to-male courtship.
2009,
Pubmed
Müller,
Differential effects of indole and aliphatic glucosinolates on lepidopteran herbivores.
2010,
Pubmed
Müller,
Taste detection of the non-volatile isothiocyanate moringin results in deterrence to glucosinolate-adapted insect larvae.
2015,
Pubmed
Nakagawa,
Functional assays for insect olfactory receptors in Xenopus oocytes.
2013,
Pubmed
,
Xenbase
Nguyen,
IQ-TREE: a fast and effective stochastic algorithm for estimating maximum-likelihood phylogenies.
2015,
Pubmed
Ning,
Functional validation of the carbon dioxide receptor in labial palps of Helicoverpa armigera moths.
2016,
Pubmed
,
Xenbase
Ozaki,
A gustatory receptor involved in host plant recognition for oviposition of a swallowtail butterfly.
2011,
Pubmed
Reiter,
Spatiotemporal Coding of Individual Chemicals by the Gustatory System.
2015,
Pubmed
Rozewicki,
MAFFT-DASH: integrated protein sequence and structural alignment.
2019,
Pubmed
Scott,
Gustatory Processing in Drosophila melanogaster.
2018,
Pubmed
Sikkink,
Genomic adaptation to agricultural environments: cabbage white butterflies (Pieris rapae) as a case study.
2017,
Pubmed
Sung,
Heterogeneity in the Drosophila gustatory receptor complexes that detect aversive compounds.
2017,
Pubmed
van Ohlen,
Cyanide detoxification in an insect herbivore: Molecular identification of β-cyanoalanine synthases from Pieris rapae.
2016,
Pubmed
Wada-Katsumata,
Changes in taste neurons support the emergence of an adaptive behavior in cockroaches.
2013,
Pubmed
Wanner,
The gustatory receptor family in the silkworm moth Bombyx mori is characterized by a large expansion of a single lineage of putative bitter receptors.
2008,
Pubmed
Weiss,
The molecular and cellular basis of bitter taste in Drosophila.
2011,
Pubmed
Wittstock,
Successful herbivore attack due to metabolic diversion of a plant chemical defense.
2004,
Pubmed
Xu,
CO2 per se activates carbon dioxide receptors.
2020,
Pubmed
,
Xenbase
You,
A heterozygous moth genome provides insights into herbivory and detoxification.
2013,
Pubmed
Zhang,
A determining factor for insect feeding preference in the silkworm, Bombyx mori.
2019,
Pubmed
