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BACKGROUND: The craniofacial skeleton is an evolutionary innovation of vertebrates. Due to its complexity and importance to protect the brain and aid in essential functions (e.g., feeding), its development requires a precisely tuned sequence of chondrification and/or ossification events. The comparison of sequential patterns of cartilage formation bears important insights into the evolution of development. Discoglossus scovazzi is a basal anuran species. The comparison of its chondrocranium (cartilaginous neuro- & viscerocranium) development with other basal anurans (Xenopus laevis, Bombina orientalis) will help establishing the ancestral pattern of chondrification sequences in anurans and will serve as basis for further studies to reconstruct ancestral conditions in amphibians, tetrapods, and vertebrates. Furthermore, evolutionary patterns in anurans can be studied in the light of adaptations once the ancestral sequence is established.
RESULTS: We present a comprehensive overview on the chondrocranium development of D. scovazzi. With clearing and staining, histology and 3D reconstructions we tracked the chondrification of 44 elements from the first mesenchymal Anlagen to the premetamorphic cartilaginous headskeleton and illustrate the sequential changes of the skull. We identified several anuran and discoglossoid traits of cartilage development. In D. scovazzi the mandibular, hyoid, and first branchial arch Anlagen develop first followed by stepwise addition of the branchial arches II, III, and IV. Nonetheless, there is no strict anterior to posterior chondrification pattern within the viscerocranium of D. scovazzi. Single hyoid arch elements chondrify after elements of the branchial arch and mandibular arch elements chondrify after elements of the branchial arch I.
CONCLUSIONS: In Osteichthyes, neurocranial elements develop in anterior to posterior direction. In the anurans investigated so far, as well as in D. scovazzi, the posterior parts of the neurocranium extend anteriorly, while the anterior parts of the neurocranium, extend posteriorly until both parts meet and fuse. Anuran cartilaginous development differs in at least two crucial traits from other gnathostomes which further supports the urgent need for more developmental investigations among this clade to understand the evolution of cartilage development in vertebrates.
Fig. 1 Cladogram showing the relationship of extant anuran families for the paraphyletic suborders Archeobatrachia and Mesobatrachia as well as the position of the three species studied here. The Neobatrachia are the most modern and monophyletic anuran suborder and comprise by far the most extant frog species. The relationships are based on Hime et al. [36]
Analía Púgener,
Osteology and skeletal development of Discoglossus sardus (Anura:Discoglossidae).
1997, Pubmed
Analía Púgener,
Osteology and skeletal development of Discoglossus sardus (Anura:Discoglossidae).
1997,
Pubmed
Cardona,
TrakEM2 software for neural circuit reconstruction.
2012,
Pubmed
Cerny,
Developmental origins and evolution of jaws: new interpretation of "maxillary" and "mandibular".
2004,
Pubmed
Compagnucci,
Pattern and polarity in the development and evolution of the gnathostome jaw: both conservation and heterotopy in the branchial arches of the shark, Scyliorhinus canicula.
2013,
Pubmed
Dingerkus,
Enzyme clearing of alcian blue stained whole small vertebrates for demonstration of cartilage.
1977,
Pubmed
Dupret,
A primitive placoderm sheds light on the origin of the jawed vertebrate face.
2014,
Pubmed
Dutel,
Neurocranial development of the coelacanth and the evolution of the sarcopterygian head.
2019,
Pubmed
Gillis,
A timeline of pharyngeal endoskeletal condensation and differentiation in the shark, Scyliorhinus canicula, and the paddlefish, Polyodon spathula.
2012,
Pubmed
Gillis,
Chondrogenesis and homology of the visceral skeleton in the little skate, Leucoraja erinacea (Chondrichthyes: Batoidea).
2009,
Pubmed
Haas,
Phylogeny of frogs as inferred from primarily larval characters (Amphibia:Anura).
2003,
Pubmed
Hernández-Jaimes,
Embryonic development of the skull of the Andean lizard Ptychoglossus bicolor (Squamata, Gymnophthalmidae).
2012,
Pubmed
Hime,
Phylogenomics Reveals Ancient Gene Tree Discordance in the Amphibian Tree of Life.
2021,
Pubmed
Hüppi,
Evolution and development of the bird chondrocranium.
2021,
Pubmed
Královec,
Development of the ethmoidal structures of the endocranium in Discoglossus pictus (Anura: Discoglossidae).
2010,
Pubmed
Kuratani,
Evolution of the vertebrate jaw from developmental perspectives.
2012,
Pubmed
Langille,
Development of the head skeleton of the Japanese medaka, Oryzias latipes (Teleostei).
1987,
Pubmed
Lukas,
Sequence of chondrocranial development in the oriental fire bellied toad Bombina orientalis.
2020,
Pubmed
Lukas,
Sequence and timing of early cranial skeletal development in Xenopus laevis.
2018,
Pubmed
,
Xenbase
McClearn,
Ontogeny of architectural complexity in embryonic quail visceral arch muscles.
1988,
Pubmed
Miyashita,
Fishing for jaws in early vertebrate evolution: a new hypothesis of mandibular confinement.
2016,
Pubmed
Noda,
Development of cranial muscles in the actinopterygian fish Senegal bichir, Polypterus senegalus Cuvier, 1829.
2017,
Pubmed
Noden,
Differentiation of avian craniofacial muscles: I. Patterns of early regulatory gene expression and myosin heavy chain synthesis.
1999,
Pubmed
Ollonen,
Skull Development, Ossification Pattern, and Adult Shape in the Emerging Lizard Model Organism Pogona vitticeps: A Comparative Analysis With Other Squamates.
2018,
Pubmed
Olsson,
Cranial neural-crest migration and chondrogenic fate in the oriental fire-bellied toad Bombina orientalis: Defining the ancestral pattern of head development in anuran amphibians.
1996,
Pubmed
Pyron,
A large-scale phylogeny of Amphibia including over 2800 species, and a revised classification of extant frogs, salamanders, and caecilians.
2011,
Pubmed
Reiss,
Early development of chondrocranium in the tailed frog Ascaphus truei (Amphibia: Anura): implications for anuran palatoquadrate homologies.
1997,
Pubmed
Rose,
Generating, growing and transforming skeletal shape: insights from amphibian pharyngeal arch cartilages.
2009,
Pubmed
Schilling,
Musculoskeletal patterning in the pharyngeal segments of the zebrafish embryo.
1997,
Pubmed
Schindelin,
Fiji: an open-source platform for biological-image analysis.
2012,
Pubmed
Smith,
Development of craniofacial musculature in Monodelphis domestica (Marsupialia, Didelphidae).
1994,
Pubmed
Sokol,
The larval chondrocranium of Pelodytes punctatus, with a review of tadpole chondrocrania.
1981,
Pubmed
Tulenko,
Formation of the chondrocranium of Trachemys scripta (Reptilia: Testudines: Emydidae) and a comparison with other described turtle taxa.
2007,
Pubmed
Warth,
Development of the skull and pectoral girdle in Siberian sturgeon, Acipenser baerii, and Russian sturgeon, Acipenser gueldenstaedtii (Acipenseriformes: Acipenseridae).
2017,
Pubmed
Ziermann,
Cephalic muscle development in the Australian lungfish, Neoceratodus forsteri.
2018,
Pubmed
Ziermann,
Muscle development in the shark Scyliorhinus canicula: implications for the evolution of the gnathostome head and paired appendage musculature.
2017,
Pubmed
Ziermann,
Analyzing developmental sequences with Parsimov--a case study of cranial muscle development in anuran larvae.
2014,
Pubmed
,
Xenbase
