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The unfolded protein response (UPR) is a wide-ranging cellular response to accumulation of malfolded proteins in the endoplasmic reticulum (ER) and acts as a quality control mechanism to halt protein processing and repair/destroy malfolded proteins under stress conditions of many kinds. Among vertebrate species, amphibians experience the greatest challenges in maintaining water and osmotic balance, the high permeability of their skin making them very susceptible to dehydration and challenging their ability to maintain cellular homeostasis. The present study evaluates the involvement of the UPR in dealing with dehydration-mediated disruption of protein processing in the tissues of African clawed frogs, Xenopus laevis. This primarily aquatic frog must deal with seasonal drought conditions in its native southern Africa environment. Key markers of cellular stress that impact protein processing were identified in six tissues of frogs that had lost 28% of total body water, as compared with fully hydrated controls. This included upregulation of glucose-regulated proteins (GRPs) that are resident chaperones in the ER, particularly 2-ninefold increases in GRP58, GRP75, and/or GRP94 in the lung and skin. Activating transcription factors (ATF3, ATF4, ATF6) that mediate UPR responses also responded to dehydration stress, particularly in skeletal muscle where both ATF3 and ATF4 rose strongly in the nucleus. Other protein markers of the UPR including GADD34, GADD153, EDEM, and XBP-1 also showed selective upregulation in frog tissues in response to dehydration and nuclear levels of the transcription factors XBP-1 and P-CREB rose indicating up-regulation of genes under their control.
Balinsky,
Amino acid metabolism and urea synthesis in naturally aestivating Xenopus laevis.
1967, Pubmed,
Xenbase
Balinsky,
Amino acid metabolism and urea synthesis in naturally aestivating Xenopus laevis.
1967,
Pubmed
,
Xenbase
Biggar,
Identification of a novel dehydration responsive gene, drp10, from the African clawed frog, Xenopus laevis.
2015,
Pubmed
,
Xenbase
Brush,
Growth arrest and DNA damage-inducible protein GADD34 targets protein phosphatase 1 alpha to the endoplasmic reticulum and promotes dephosphorylation of the alpha subunit of eukaryotic translation initiation factor 2.
2003,
Pubmed
Cartledge,
Water balance of field-excavated aestivating Australian desert frogs, the cocoon-forming Neobatrachus aquilonius and the non-cocooning Notaden nichollsi (Amphibia: Myobatrachidae).
2006,
Pubmed
Carvalho,
The molecular chaperone binding protein BiP prevents leaf dehydration-induced cellular homeostasis disruption.
2014,
Pubmed
Chakrabarti,
A review of the mammalian unfolded protein response.
2011,
Pubmed
do Amaral,
Hepatic transcriptome of the freeze-tolerant Cope's gray treefrog, Dryophytes chrysoscelis: responses to cold acclimation and freezing.
2020,
Pubmed
Dores-Silva,
Human mitochondrial Hsp70 (mortalin): shedding light on ATPase activity, interaction with adenosine nucleotides, solution structure and domain organization.
2015,
Pubmed
Dragosits,
The response to unfolded protein is involved in osmotolerance of Pichia pastoris.
2010,
Pubmed
Dubaissi,
Functional characterization of the mucus barrier on the Xenopus tropicalis skin surface.
2018,
Pubmed
,
Xenbase
Flachbartová,
Mortalin - a multipotent chaperone regulating cellular processes ranging from viral infection to neurodegeneration.
2013,
Pubmed
Fusakio,
Transcription factor ATF4 directs basal and stress-induced gene expression in the unfolded protein response and cholesterol metabolism in the liver.
2016,
Pubmed
Gething,
Role and regulation of the ER chaperone BiP.
1999,
Pubmed
Giraud-Billoud,
Twenty years of the 'Preparation for Oxidative Stress' (POS) theory: Ecophysiological advantages and molecular strategies.
2019,
Pubmed
Harding,
An integrated stress response regulates amino acid metabolism and resistance to oxidative stress.
2003,
Pubmed
Hillman,
Anuran amphibians as comparative models for understanding extreme dehydration tolerance: a unique negative feedback lymphatic mechanism for blood volume regulation.
2018,
Pubmed
Hoffman,
Protein disulfide isomerase-endoplasmic reticulum resident protein 57 regulates allergen-induced airways inflammation, fibrosis, and hyperresponsiveness.
2016,
Pubmed
Kikuchi,
CREB is activated by ER stress and modulates the unfolded protein response by regulating the expression of IRE1α and PERK.
2016,
Pubmed
Kozutsumi,
The presence of malfolded proteins in the endoplasmic reticulum signals the induction of glucose-regulated proteins.
1988,
Pubmed
Krivoruchko,
Activation of the unfolded protein response during anoxia exposure in the turtle Trachemys scripta elegans.
2013,
Pubmed
Lee,
Structure of the dual enzyme Ire1 reveals the basis for catalysis and regulation in nonconventional RNA splicing.
2008,
Pubmed
Lee,
The glucose-regulated proteins: stress induction and clinical applications.
2001,
Pubmed
Luo,
Induction of Grp78/BiP by translational block: activation of the Grp78 promoter by ATF4 through and upstream ATF/CRE site independent of the endoplasmic reticulum stress elements.
2003,
Pubmed
Luu,
The regulation of heat shock proteins in response to dehydration in Xenopus laevis.
2018,
Pubmed
,
Xenbase
Malik,
Activation of extracellular signal-regulated kinases during dehydration in the African clawed frog, Xenopus laevis.
2009,
Pubmed
,
Xenbase
Malik,
Transcriptional regulation of antioxidant enzymes by FoxO1 under dehydration stress.
2011,
Pubmed
,
Xenbase
Malik,
Activation of antioxidant defense during dehydration stress in the African clawed frog.
2009,
Pubmed
,
Xenbase
Mamady,
Up-regulation of the endoplasmic reticulum molecular chaperone GRP78 during hibernation in thirteen-lined ground squirrels.
2006,
Pubmed
McQuiston,
Recent insights into PERK-dependent signaling from the stressed endoplasmic reticulum.
2017,
Pubmed
Molinari,
Role of EDEM in the release of misfolded glycoproteins from the calnexin cycle.
2003,
Pubmed
Muir,
Osmotic and metabolic responses to dehydration and urea-loading in a dormant, terrestrially hibernating frog.
2007,
Pubmed
Ni,
ER chaperones in mammalian development and human diseases.
2007,
Pubmed
Park,
Endoplasmic Reticulum Plays a Critical Role in Integrating Signals Generated by Both Biotic and Abiotic Stress in Plants.
2019,
Pubmed
Schröder,
Endoplasmic reticulum stress responses.
2008,
Pubmed
Schröder,
The mammalian unfolded protein response.
2005,
Pubmed
Shishkin,
AGR2, ERp57/GRP58, and some other human protein disulfide isomerases.
2013,
Pubmed
Storey,
In defense of proteins: Chaperones respond to freezing, anoxia, or dehydration stress in tissues of freeze tolerant wood frogs.
2019,
Pubmed
Storey,
Metabolic rate depression in animals: transcriptional and translational controls.
2004,
Pubmed
Storey,
Life in the slow lane: molecular mechanisms of estivation.
2002,
Pubmed
Storey,
Molecular Physiology of Freeze Tolerance in Vertebrates.
2017,
Pubmed
Storey,
Tribute to P. L. Lutz: putting life on 'pause'--molecular regulation of hypometabolism.
2007,
Pubmed
Turano,
ERp57/GRP58: a protein with multiple functions.
2011,
Pubmed
Varga,
Frog Skin Innate Immune Defences: Sensing and Surviving Pathogens.
2018,
Pubmed
,
Xenbase
Wang,
Grass carp (Ctenopharyngodon idella) ATF6 (activating transcription factor 6) modulates the transcriptional level of GRP78 and GRP94 in CIK cells.
2016,
Pubmed
Wright,
The crab-eating frog, Rana cancrivora, up-regulates hepatic carbamoyl phosphate synthetase I activity and tissue osmolyte levels in response to increased salinity.
2004,
Pubmed
Wu,
Regulation of the insulin-Akt signaling pathway and glycolysis during dehydration stress in the African clawed frog Xenopus laevis.
2017,
Pubmed
,
Xenbase
Wu,
Dehydration mediated microRNA response in the African clawed frog Xenopus laevis.
2013,
Pubmed
,
Xenbase
Yancey,
Organic osmolytes as compatible, metabolic and counteracting cytoprotectants in high osmolarity and other stresses.
2005,
Pubmed
Yoshida,
XBP1 mRNA is induced by ATF6 and spliced by IRE1 in response to ER stress to produce a highly active transcription factor.
2001,
Pubmed
Zhang,
FoxO4 activity is regulated by phosphorylation and the cellular environment during dehydration in the African clawed frog, Xenopus laevis.
2018,
Pubmed
,
Xenbase
Zhu,
Role of the unfolded protein response, GRP78 and GRP94 in organ homeostasis.
2015,
Pubmed
