Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
???displayArticle.abstract???
During canonical Wnt signaling, the Wnt receptor complex is sequestered together with glycogen synthase kinase 3 (GSK3) and Axin inside late endosomes, known as multivesicular bodies (MVBs). Here, we present experiments showing that Wnt causes the endocytosis of focal adhesion (FA) proteins and depletion of Integrin β 1 (ITGβ1) from the cell surface. FAs and integrins link the cytoskeleton to the extracellular matrix. Wnt-induced endocytosis caused ITGβ1 depletion from the plasma membrane and was accompanied by striking changes in the actin cytoskeleton. In situ protease protection assays in cultured cells showed that ITGβ1 was sequestered within membrane-bounded organelles that corresponded to Wnt-induced MVBs containing GSK3 and FA-associated proteins. An in vivo model using Xenopus embryos dorsalized by Wnt8 mRNA showed that ITGβ1 depletion decreased Wnt signaling. The finding of a crosstalk between two major signaling pathways, canonical Wnt and focal adhesions, should be relevant to human cancer and cell biology.
Abercrombie,
The locomotion of fibroblasts in culture. IV. Electron microscopy of the leading lamella.
1971, Pubmed
Abercrombie,
The locomotion of fibroblasts in culture. IV. Electron microscopy of the leading lamella.
1971,
Pubmed
Acebron,
Mitotic wnt signaling promotes protein stabilization and regulates cell size.
2014,
Pubmed
Albrecht,
Arginine methylation is required for canonical Wnt signaling and endolysosomal trafficking.
2018,
Pubmed
Albrecht,
GSK3 Inhibits Macropinocytosis and Lysosomal Activity through the Wnt Destruction Complex Machinery.
2020,
Pubmed
,
Xenbase
Albrecht,
Cell Biology of Canonical Wnt Signaling.
2021,
Pubmed
Bachir,
Actin-Based Adhesion Modules Mediate Cell Interactions with the Extracellular Matrix and Neighboring Cells.
2017,
Pubmed
Beckerle,
Identification of a new protein localized at sites of cell-substrate adhesion.
1986,
Pubmed
Bianchi,
Regulation of FAK Ser-722 phosphorylation and kinase activity by GSK3 and PP1 during cell spreading and migration.
2005,
Pubmed
Bilic,
Wnt induces LRP6 signalosomes and promotes dishevelled-dependent LRP6 phosphorylation.
2007,
Pubmed
Brower,
Molecular evolution of integrins: genes encoding integrin beta subunits from a coral and a sponge.
1997,
Pubmed
Cai,
Glycogen synthase kinase 3- and extracellular signal-regulated kinase-dependent phosphorylation of paxillin regulates cytoskeletal rearrangement.
2006,
Pubmed
Capelluto,
The DIX domain targets dishevelled to actin stress fibres and vesicular membranes.
2002,
Pubmed
,
Xenbase
Chaturvedi,
Molecular dissection of the mechanism by which EWS/FLI expression compromises actin cytoskeletal integrity and cell adhesion in Ewing sarcoma.
2014,
Pubmed
Chen,
fastp: an ultra-fast all-in-one FASTQ preprocessor.
2018,
Pubmed
Colozza,
Wnt-inducible Lrp6-APEX2 interacting proteins identify ESCRT machinery and Trk-fused gene as components of the Wnt signaling pathway.
2020,
Pubmed
,
Xenbase
Condon,
Macropinosome formation by tent pole ruffling in macrophages.
2018,
Pubmed
Ding,
Bighead is a Wnt antagonist secreted by the Xenopus Spemann organizer that promotes Lrp6 endocytosis.
2018,
Pubmed
,
Xenbase
Dobrowolski,
Presenilin deficiency or lysosomal inhibition enhances Wnt signaling through relocalization of GSK3 to the late-endosomal compartment.
2012,
Pubmed
Doherty,
Mechanisms of endocytosis.
2009,
Pubmed
Du,
Extracellular matrix stiffness dictates Wnt expression through integrin pathway.
2016,
Pubmed
Frame,
v-Src's hold over actin and cell adhesions.
2002,
Pubmed
Gallego-Muñoz,
Human corneal fibroblast migration and extracellular matrix synthesis during stromal repair: Role played by platelet-derived growth factor-BB, basic fibroblast growth factor, and transforming growth factor-β1.
2018,
Pubmed
Griffith,
RNAi knockdown of the focal adhesion protein TES reveals its role in actin stress fibre organisation.
2005,
Pubmed
Gruenberg,
The biogenesis of multivesicular endosomes.
2004,
Pubmed
Gu,
Integrins traffic rapidly via circular dorsal ruffles and macropinocytosis during stimulated cell migration.
2011,
Pubmed
Kaplan,
Association of the amino-terminal half of c-Src with focal adhesions alters their properties and is regulated by phosphorylation of tyrosine 527.
1994,
Pubmed
Kim,
Identification of targets of the Wnt pathway destruction complex in addition to beta-catenin.
2009,
Pubmed
,
Xenbase
Kim,
Wnt Signaling Translocates Lys48-Linked Polyubiquitinated Proteins to the Lysosomal Pathway.
2015,
Pubmed
Li,
TES inhibits colorectal cancer progression through activation of p38.
2016,
Pubmed
Li,
Macropinocytosis-mediated membrane recycling drives neural crest migration by delivering F-actin to the lamellipodium.
2020,
Pubmed
Loh,
Generating Cellular Diversity and Spatial Form: Wnt Signaling and the Evolution of Multicellular Animals.
2016,
Pubmed
Moreno-Layseca,
Integrin trafficking in cells and tissues.
2019,
Pubmed
Mylvaganam,
The cytoskeleton in phagocytosis and macropinocytosis.
2021,
Pubmed
Nichols,
Endocytosis without clathrin coats.
2001,
Pubmed
Niehrs,
The complex world of WNT receptor signalling.
2012,
Pubmed
Niehrs,
The role of Xenopus developmental biology in unraveling Wnt signalling and antero-posterior axis formation.
2022,
Pubmed
,
Xenbase
Nieto,
EMT: 2016.
2016,
Pubmed
Nusse,
Wnt/β-Catenin Signaling, Disease, and Emerging Therapeutic Modalities.
2017,
Pubmed
Ramirez,
Plasma membrane V-ATPase controls oncogenic RAS-induced macropinocytosis.
2019,
Pubmed
Ramirez,
The α₂β₁ integrin is a metastasis suppressor in mouse models and human cancer.
2011,
Pubmed
Redelman-Sidi,
The Canonical Wnt Pathway Drives Macropinocytosis in Cancer.
2018,
Pubmed
Retta,
beta1-integrin cytoplasmic subdomains involved in dominant negative function.
1998,
Pubmed
Smith,
Xenopus cell lines.
1991,
Pubmed
,
Xenbase
Sun,
Glycogen synthase kinase 3 in the world of cell migration.
2009,
Pubmed
Swanson,
The breadth of macropinocytosis research.
2019,
Pubmed
Taelman,
Wnt signaling requires sequestration of glycogen synthase kinase 3 inside multivesicular endosomes.
2010,
Pubmed
,
Xenbase
Tejeda-Muñoz,
Wnt canonical pathway activates macropinocytosis and lysosomal degradation of extracellular proteins.
2019,
Pubmed
Tu,
Endosomal-sorting complexes required for transport (ESCRT) pathway-dependent endosomal traffic regulates the localization of active Src at focal adhesions.
2010,
Pubmed
Vanlandingham,
Rab7 regulates late endocytic trafficking downstream of multivesicular body biogenesis and cargo sequestration.
2009,
Pubmed
Vinyoles,
Multivesicular GSK3 sequestration upon Wnt signaling is controlled by p120-catenin/cadherin interaction with LRP5/6.
2014,
Pubmed
Yokoyama,
Dishevelled-2 docks and activates Src in a Wnt-dependent manner.
2009,
Pubmed
Yu,
Kindlin 2 forms a transcriptional complex with β-catenin and TCF4 to enhance Wnt signalling.
2012,
Pubmed
