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During amphibian metamorphosis which is triggered by thyroid hormone (TH), the small intestine is extensively remodeled from the larval to adult form. In the Xenopus laevis intestine, some of the larval epithelial cells dedifferentiate into adult stem cells, which newly form the adult epithelium similar to the mammalian one. We have previously shown that TH-activated Shh, Wnt and Notch signaling pathways play important roles in adult epithelial development. Here we focus on the Hippo signaling pathway, which is known to interact with these pathways in the mammalian intestine. Our quantitative RT-PCR analysis indicates that the expression of genes involved in this pathway including YAP1, TAZ, TEAD1 and core kinases is differently regulated by TH in the metamorphosing intestine. Additionally, we show by in situ hybridization and immunohistochemistry that the transcriptional co-activator YAP1, a major effector of the Hippo signaling, is expressed in the adult stem cells and connective tissue cells surrounding them and that YAP1 protein is localized in either nucleus or cytoplasm of the stem cells. We further show that YAP1 binds its binding partner TEAD1 (transcription factor) in vivo and that their interaction is inhibited by verteporfin (VP). More importantly, by using VP in organ culture of the tadpoleintestine, we experimentally demonstrate that the inhibition of YAP1-TEAD1 interaction decreases both TH-induced stem cells expressing LGR5 and nearby connective tissue cells in number and proliferation, leading to the failure of adult epithelial development. Our results indicate that YAP-TEAD complex is required for stem cell development during intestinal remodeling.
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Aoki,
Foxl1-expressing mesenchymal cells constitute the intestinal stem cell niche.
2016,
Pubmed
Ayyaz,
Single-cell transcriptomes of the regenerating intestine reveal a revival stem cell.
2019,
Pubmed
Barry,
Restriction of intestinal stem cell expansion and the regenerative response by YAP.
2013,
Pubmed
Bjerknes,
The stem-cell zone of the small intestinal epithelium. III. Evidence from columnar, enteroendocrine, and mucous cells in the adult mouse.
1981,
Pubmed
Brodowska,
The clinically used photosensitizer Verteporfin (VP) inhibits YAP-TEAD and human retinoblastoma cell growth in vitro without light activation.
2014,
Pubmed
Buchholz,
Pairing morphology with gene expression in thyroid hormone-induced intestinal remodeling and identification of a core set of TH-induced genes across tadpole tissues.
2007,
Pubmed
,
Xenbase
Cai,
The Hippo signaling pathway restricts the oncogenic potential of an intestinal regeneration program.
2010,
Pubmed
Camargo,
YAP1 increases organ size and expands undifferentiated progenitor cells.
2007,
Pubmed
Cao,
YAP regulates neural progenitor cell number via the TEA domain transcription factor.
2008,
Pubmed
Cheng,
Whole population cell kinetics and postnatal development of the mouse intestinal epithelium.
1985,
Pubmed
Das,
Identification of direct thyroid hormone response genes reveals the earliest gene regulation programs during frog metamorphosis.
2009,
Pubmed
,
Xenbase
DeLay,
Tissue-Specific Gene Inactivation in Xenopus laevis: Knockout of lhx1 in the Kidney with CRISPR/Cas9.
2018,
Pubmed
,
Xenbase
Fevr,
Wnt/beta-catenin is essential for intestinal homeostasis and maintenance of intestinal stem cells.
2007,
Pubmed
Fu,
A causative role of stromelysin-3 in extracellular matrix remodeling and epithelial apoptosis during intestinal metamorphosis in Xenopus laevis.
2005,
Pubmed
,
Xenbase
Fujimoto,
Expression of hyaluronan synthases upregulated by thyroid hormone is involved in intestinal stem cell development during Xenopus laevis metamorphosis.
2018,
Pubmed
,
Xenbase
Gregorieff,
Yap-dependent reprogramming of Lgr5(+) stem cells drives intestinal regeneration and cancer.
2015,
Pubmed
Hasebe,
Thyroid hormone-induced expression of Foxl1 in subepithelial fibroblasts correlates with adult stem cell development during Xenopus intestinal remodeling.
2020,
Pubmed
,
Xenbase
Hasebe,
Thyroid Hormone-Induced Activation of Notch Signaling is Required for Adult Intestinal Stem Cell Development During Xenopus Laevis Metamorphosis.
2017,
Pubmed
,
Xenbase
Hasebe,
Thyroid hormone activates Wnt/β-catenin signaling involved in adult epithelial development during intestinal remodeling in Xenopus laevis.
2016,
Pubmed
,
Xenbase
Hasebe,
Essential Roles of Thyroid Hormone-Regulated Hyaluronan/CD44 Signaling in Adult Stem Cell Development During Xenopus laevis Intestinal Remodeling.
2017,
Pubmed
,
Xenbase
Hasebe,
Evidence for a cooperative role of gelatinase A and membrane type-1 matrix metalloproteinase during Xenopus laevis development.
2007,
Pubmed
,
Xenbase
Hasebe,
Spatial and temporal expression profiles suggest the involvement of gelatinase A and membrane type 1 matrix metalloproteinase in amphibian metamorphosis.
2006,
Pubmed
,
Xenbase
Hasebe,
Thyroid hormone-induced sonic hedgehog signal up-regulates its own pathway in a paracrine manner in the Xenopus laevis intestine during metamorphosis.
2012,
Pubmed
,
Xenbase
Hasebe,
Thyroid hormone-regulated expression of nuclear lamins correlates with dedifferentiation of intestinal epithelial cells during Xenopus laevis metamorphosis.
2011,
Pubmed
,
Xenbase
Hong,
The Hippo pathway in intestinal regeneration and disease.
2016,
Pubmed
Imajo,
A molecular mechanism that links Hippo signalling to the inhibition of Wnt/β-catenin signalling.
2012,
Pubmed
,
Xenbase
Ishizuya-Oka,
Regeneration of the amphibian intestinal epithelium under the control of stem cell niche.
2007,
Pubmed
,
Xenbase
Ishizuya-Oka,
Origin of the adult intestinal stem cells induced by thyroid hormone in Xenopus laevis.
2009,
Pubmed
,
Xenbase
Ishizuya-Oka,
Shh/BMP-4 signaling pathway is essential for intestinal epithelial development during Xenopus larval-to-adult remodeling.
2006,
Pubmed
,
Xenbase
Ishizuya-Oka,
Requirement for matrix metalloproteinase stromelysin-3 in cell migration and apoptosis during tissue remodeling in Xenopus laevis.
2000,
Pubmed
,
Xenbase
Ishizuya-Oka,
Thyroid hormone regulation of stem cell development during intestinal remodeling.
2008,
Pubmed
,
Xenbase
Ishizuya-Oka,
Thyroid hormone-upregulated expression of Musashi-1 is specific for progenitor cells of the adult epithelium during amphibian gastrointestinal remodeling.
2003,
Pubmed
,
Xenbase
Ishizuya-Oka,
Induction of metamorphosis by thyroid hormone in anuran small intestine cultured organotypically in vitro.
1991,
Pubmed
,
Xenbase
Ishizuya-Oka,
Apoptosis and cell proliferation in the Xenopus small intestine during metamorphosis.
1996,
Pubmed
,
Xenbase
Johnson,
The two faces of Hippo: targeting the Hippo pathway for regenerative medicine and cancer treatment.
2014,
Pubmed
Kaestner,
The Intestinal Stem Cell Niche: A Central Role for Foxl1-Expressing Subepithelial Telocytes.
2019,
Pubmed
Kanamori,
The regulation of thyroid hormone receptor beta genes by thyroid hormone in Xenopus laevis.
1992,
Pubmed
,
Xenbase
Konsavage,
Wnt/β-catenin signaling regulates Yes-associated protein (YAP) gene expression in colorectal carcinoma cells.
2012,
Pubmed
Kress,
Thyroid hormones and the control of cell proliferation or cell differentiation: paradox or duality?
2009,
Pubmed
Lian,
The role of YAP transcription coactivator in regulating stem cell self-renewal and differentiation.
2010,
Pubmed
Liu-Chittenden,
Genetic and pharmacological disruption of the TEAD-YAP complex suppresses the oncogenic activity of YAP.
2012,
Pubmed
Morin-Kensicki,
Defects in yolk sac vasculogenesis, chorioallantoic fusion, and embryonic axis elongation in mice with targeted disruption of Yap65.
2006,
Pubmed
Moya,
Hippo-YAP/TAZ signalling in organ regeneration and regenerative medicine.
2019,
Pubmed
Piccolo,
The biology of YAP/TAZ: hippo signaling and beyond.
2014,
Pubmed
Ramos,
The Hippo signaling pathway and stem cell biology.
2012,
Pubmed
Rosenbluh,
β-Catenin-driven cancers require a YAP1 transcriptional complex for survival and tumorigenesis.
2012,
Pubmed
Sato,
Growing self-organizing mini-guts from a single intestinal stem cell: mechanism and applications.
2013,
Pubmed
Schmitt,
CD44 functions in Wnt signaling by regulating LRP6 localization and activation.
2015,
Pubmed
,
Xenbase
Session,
Genome evolution in the allotetraploid frog Xenopus laevis.
2016,
Pubmed
,
Xenbase
Shi,
The earliest changes in gene expression in tadpole intestine induced by thyroid hormone.
1993,
Pubmed
,
Xenbase
Shi,
Biphasic intestinal development in amphibians: embryogenesis and remodeling during metamorphosis.
1996,
Pubmed
,
Xenbase
Shi,
Cloning and characterization of the ribosomal protein L8 gene from Xenopus laevis.
1994,
Pubmed
,
Xenbase
Sun,
Expression profiling of intestinal tissues implicates tissue-specific genes and pathways essential for thyroid hormone-induced adult stem cell development.
2013,
Pubmed
,
Xenbase
Taniguchi,
A gp130-Src-YAP module links inflammation to epithelial regeneration.
2015,
Pubmed
Tata,
Gene expression during metamorphosis: an ideal model for post-embryonic development.
1993,
Pubmed
Vassilev,
TEAD/TEF transcription factors utilize the activation domain of YAP65, a Src/Yes-associated protein localized in the cytoplasm.
2001,
Pubmed
Wang,
The Hippo pathway in tissue homeostasis and regeneration.
2017,
Pubmed
Wen,
A requirement for hedgehog signaling in thyroid hormone-induced postembryonic intestinal remodeling.
2015,
Pubmed
,
Xenbase
Wong,
Coordinated regulation of and transcriptional activation by Xenopus thyroid hormone and retinoid X receptors.
1995,
Pubmed
,
Xenbase
Yu,
Hippo Pathway in Organ Size Control, Tissue Homeostasis, and Cancer.
2015,
Pubmed
Zhao,
TEAD mediates YAP-dependent gene induction and growth control.
2008,
Pubmed
Zhou,
Mst1 and Mst2 protein kinases restrain intestinal stem cell proliferation and colonic tumorigenesis by inhibition of Yes-associated protein (Yap) overabundance.
2011,
Pubmed
