Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
???displayArticle.abstract???
The NRT1/PTR FAMILY (NPF) in Arabidopsis (Arabidopsis thaliana) plays a major role as a nitrate transporter. The first nitrate transporter activity biosensor NiTrac1 converted the dual-affinity nitrate transceptor NPF6.3 into fluorescence activity sensors. To test whether this approach is transferable to other members of this family, screening for genetically encoded fluorescence transport activity sensor was performed with the member of the NPF family in Arabidopsis. In this study, NPF1.3, an uncharacterized member of NPF in Arabidopsis, was converted into a transporter activity biosensor NiTrac-NPF1.3 that responds specifically to nitrate. The emission ratio change of NiTrac-NPF1.3 triggered by the addition of nitrate reveals the important function of NPF1.3 in nitrate transport in Arabidopsis. A functional analysis of Xenopus laevis oocytes confirmed that NPF1.3 plays a role as a nitrate transporter. This new technology is applicable in plant and medical research.
Almagro,
Characterization of the Arabidopsis nitrate transporter NRT1.6 reveals a role of nitrate in early embryo development.
2008, Pubmed,
Xenbase
Almagro,
Characterization of the Arabidopsis nitrate transporter NRT1.6 reveals a role of nitrate in early embryo development.
2008,
Pubmed
,
Xenbase
Ast,
Ratiometric Matryoshka biosensors from a nested cassette of green- and orange-emitting fluorescent proteins.
2017,
Pubmed
Bermejo,
Dynamic analysis of cytosolic glucose and ATP levels in yeast using optical sensors.
2010,
Pubmed
Bermejo,
In vivo biochemistry: quantifying ion and metabolite levels in individual cells or cultures of yeast.
2011,
Pubmed
Bermejo,
Optical sensors for measuring dynamic changes of cytosolic metabolite levels in yeast.
2011,
Pubmed
Boursiac,
ABA transport and transporters.
2013,
Pubmed
Branco-Price,
Genome-wide analysis of transcript abundance and translation in Arabidopsis seedlings subjected to oxygen deprivation.
2005,
Pubmed
Chaudhuri,
Dynamic imaging of glucose flux impedance using FRET sensors in wild-type Arabidopsis plants.
2011,
Pubmed
Chaudhuri,
Protonophore- and pH-insensitive glucose and sucrose accumulation detected by FRET nanosensors in Arabidopsis root tips.
2008,
Pubmed
Chen,
Potential transceptor AtNRT1.13 modulates shoot architecture and flowering time in a nitrate-dependent manner.
2021,
Pubmed
Chiang,
Mechanisms and functional properties of two peptide transporters, AtPTR2 and fPTR2.
2004,
Pubmed
,
Xenbase
Chiu,
Mutation of a nitrate transporter, AtNRT1:4, results in a reduced petiole nitrate content and altered leaf development.
2004,
Pubmed
,
Xenbase
De Michele,
Fluorescent sensors reporting the activity of ammonium transceptors in live cells.
2013,
Pubmed
Deuschle,
Rapid metabolism of glucose detected with FRET glucose nanosensors in epidermal cells and intact roots of Arabidopsis RNA-silencing mutants.
2006,
Pubmed
Doki,
Structural basis for dynamic mechanism of proton-coupled symport by the peptide transporter POT.
2013,
Pubmed
Fan,
The Arabidopsis nitrate transporter NRT1.7, expressed in phloem, is responsible for source-to-sink remobilization of nitrate.
2009,
Pubmed
Forde,
Local and long-range signaling pathways regulating plant responses to nitrate.
2002,
Pubmed
Gietz,
Improved method for high efficiency transformation of intact yeast cells.
1992,
Pubmed
Hammes,
Functional properties of the Arabidopsis peptide transporters AtPTR1 and AtPTR5.
2010,
Pubmed
,
Xenbase
He,
Tonoplast-localized nitrate uptake transporters involved in vacuolar nitrate efflux and reallocation in Arabidopsis.
2017,
Pubmed
,
Xenbase
Ho,
Fluorescent sensors for activity and regulation of the nitrate transceptor CHL1/NRT1.1 and oligopeptide transporters.
2014,
Pubmed
,
Xenbase
Hsu,
Two phloem nitrate transporters, NRT1.11 and NRT1.12, are important for redistributing xylem-borne nitrate to enhance plant growth.
2013,
Pubmed
,
Xenbase
Huang,
Cloning and functional characterization of an Arabidopsis nitrate transporter gene that encodes a constitutive component of low-affinity uptake.
1999,
Pubmed
,
Xenbase
Jones,
In vivo biochemistry: applications for small molecule biosensors in plant biology.
2013,
Pubmed
Kanno,
Identification of an abscisic acid transporter by functional screening using the receptor complex as a sensor.
2012,
Pubmed
Komarova,
AtPTR1 and AtPTR5 transport dipeptides in planta.
2008,
Pubmed
,
Xenbase
Kotur,
Nitrate transport capacity of the Arabidopsis thaliana NRT2 family members and their interactions with AtNAR2.1.
2012,
Pubmed
,
Xenbase
Krouk,
Nitrate-regulated auxin transport by NRT1.1 defines a mechanism for nutrient sensing in plants.
2010,
Pubmed
,
Xenbase
Lemaire,
Glucose and sucrose act as agonist and mannose as antagonist ligands of the G protein-coupled receptor Gpr1 in the yeast Saccharomyces cerevisiae.
2004,
Pubmed
Léran,
A unified nomenclature of NITRATE TRANSPORTER 1/PEPTIDE TRANSPORTER family members in plants.
2014,
Pubmed
Li,
Identification of a Stelar-Localized Transport Protein That Facilitates Root-to-Shoot Transfer of Chloride in Arabidopsis.
2016,
Pubmed
,
Xenbase
Li,
The Arabidopsis nitrate transporter NRT1.8 functions in nitrate removal from the xylem sap and mediates cadmium tolerance.
2010,
Pubmed
,
Xenbase
Li,
NRT1.5/NPF7.3 Functions as a Proton-Coupled H+/K+ Antiporter for K+ Loading into the Xylem in Arabidopsis.
2017,
Pubmed
,
Xenbase
Li,
AtNPF2.5 Modulates Chloride (Cl-) Efflux from Roots of Arabidopsis thaliana.
2016,
Pubmed
,
Xenbase
Lin,
Mutation of the Arabidopsis NRT1.5 nitrate transporter causes defective root-to-shoot nitrate transport.
2008,
Pubmed
,
Xenbase
Loqué,
Pore mutations in ammonium transporter AMT1 with increased electrogenic ammonium transport activity.
2009,
Pubmed
,
Xenbase
McAllister,
Engineering nitrogen use efficient crop plants: the current status.
2012,
Pubmed
Nour-Eldin,
NRT/PTR transporters are essential for translocation of glucosinolate defence compounds to seeds.
2012,
Pubmed
,
Xenbase
Okumoto,
Quantitative imaging for discovery and assembly of the metabo-regulome.
2008,
Pubmed
Okumoto,
Quantitative imaging using genetically encoded sensors for small molecules in plants.
2012,
Pubmed
Pehl,
Automated higher-throughput compound screening on ion channel targets based on the Xenopus laevis oocyte expression system.
2004,
Pubmed
,
Xenbase
Rizzo,
Optimization of pairings and detection conditions for measurement of FRET between cyan and yellow fluorescent proteins.
2006,
Pubmed
Sakakibara,
Interactions between nitrogen and cytokinin in the regulation of metabolism and development.
2006,
Pubmed
Siddiqi,
Studies of the Uptake of Nitrate in Barley: I. Kinetics of NO(3) Influx.
1990,
Pubmed
Song,
Nutritional and osmotic roles of nitrate in a euhalophyte and a xerophyte in saline conditions.
2006,
Pubmed
Tal,
The Arabidopsis NPF3 protein is a GA transporter.
2016,
Pubmed
Vidal,
A systems view of nitrogen nutrient and metabolite responses in Arabidopsis.
2008,
Pubmed
Wang,
Ammonium Uptake by Rice Roots (III. Electrophysiology).
1994,
Pubmed
Wang,
Arabidopsis nitrate transporter NRT1.9 is important in phloem nitrate transport.
2011,
Pubmed
,
Xenbase
