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Comp Med
2022 Aug 01;724:243-247. doi: 10.30802/AALAS-CM-22-000016.
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Anesthesia for Echocardiography and Magnetic Resonance Imaging in the African Clawed Frog (Xenopus laevis).
Corno AF
,
Flores NE
,
Li W
,
Gomez TH
,
Salazar JD
.
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This report describes an anesthesia technique that we used to study cardiovascular anatomy and physiology with echocardiography and cardiac magnetic resonance (CMR) in 46 African clawed frogs (Xenopus laevis) (n = 24 for electrocardiography and n = 22 for CMR). For administration of anesthesia, 3 holding tanks, one each for transportation, sedation, and recovery, were filled with filtered water, with 0.05% buffered tricaine methasulfonate solution (MS-222) added into the sedation tank. Fifteen minutes after the frog was placed in the sedation tank, a paper towel was soaked in MS-222 solution, and the frog was placed in a supine position and rolled 3 to 4 times in the soaked paper with the head and legs exposed. Vital signs were monitored and recorded throughout the procedure. After imagining, frogs were unrolled from the paper towel, placed in the recovery tank, and later returned to their home tank. Monitoring was discontinued when the frogs resumed typical activity. No mortality or complications were observed in frogs that underwent this procedure. Mean duration ±1 SD of anesthesia induction was 12 ± 5 min in the echocardiography group and 14 ± 6 min in the CMR group. The mean duration of anesthesia maintenance was 60 ± 18 min in the echocardiography group and 118 ± 37 min in the CMR group. An additional dose of anesthesia was necessary during maintenance for 9 of 24 (37%) frogs in the echocardiography group and 6 of 22 (27%) frogs in the CMR group. At the end of the procedure, the mean oxygen saturation was 66 ± 9% in the echocardiography group and 85 ± 6% in the CMR group, and heart rate was 48 ± 13 beats/min in the echocardiography group and 42 ± 7 beats/min in the CMR group. We conclude that the anesthesia technique of immersion in MS-222 is suitable for performing echocardiography and CMR imaging in this species without complications.
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Bartlett,
Echocardiographic assessment of cardiac morphology and function in Xenopus.
2010, Pubmed,
Xenbase
Bartlett,
Echocardiographic assessment of cardiac morphology and function in Xenopus.
2010,
Pubmed
,
Xenbase
Buchhorn,
Pathogenetic mechanisms of venous congestion after the Fontan procedure.
2001,
Pubmed
Burggren,
Amphibians as animal models for laboratory research in physiology.
2007,
Pubmed
,
Xenbase
CHAMBERS,
Cor triloculare biatriatum. Survival to adult life.
1961,
Pubmed
Corno,
Univentricular heart: can we alter the natural history?
1982,
Pubmed
Corno,
Assisted Fontan procedure: animal and in vitro models and computational fluid dynamics study.
2010,
Pubmed
de Leval,
Four decades of Fontan palliation.
2010,
Pubmed
Dennis,
Clinical Outcomes in Adolescents and Adults After the Fontan Procedure.
2018,
Pubmed
Gentz,
Medicine and surgery of amphibians.
2007,
Pubmed
Gewillig,
The Fontan circulation after 45 years: update in physiology.
2016,
Pubmed
Giannico,
Total extracardiac right heart bypass.
1992,
Pubmed
Guénette,
Anesthetic properties of propofol in African clawed frogs (Xenopus laevis).
2008,
Pubmed
,
Xenbase
Haberich,
The functional separation of venous and arterial blood in the univentricular frog heart.
1965,
Pubmed
Hicks,
The physiological and evolutionary significance of cardiovascular shunting patterns in reptiles.
2002,
Pubmed
Hillman,
Net cardiac shunts in anuran amphibians: physiology or physics?
2014,
Pubmed
Knight,
Use of frog ventricle to examine mechanical and electrical activity of heart.
1989,
Pubmed
Koustubhan,
Humane anesthesia and pain management in amphibian limb surgery of Rana pipiens.
2013,
Pubmed
,
Xenbase
Marcelletti,
Inferior vena cava-pulmonary artery extracardiac conduit. A new form of right heart bypass.
1990,
Pubmed
Medler,
Anesthetic MS-222 eliminates nerve and muscle activity in frogs used for physiology teaching laboratories.
2019,
Pubmed
Meyer,
Intracardiac anatomical relationships and potential for streaming in double inlet left ventricles.
2017,
Pubmed
National Research Council (US) Committee for the Update of the Guide for the
Care and Use of Laboratory Animals,
2011,
Pubmed
Paduano,
The Response of Gray Treefrogs to Anesthesia by Tricaine Methanesulfonate (TMS or MS-222).
2013,
Pubmed
Poterucha,
Survival and outcomes of patients with unoperated single ventricle.
2016,
Pubmed
Rijnberg,
Energetics of Blood Flow in Cardiovascular Disease: Concept and Clinical Implications of Adverse Energetics in Patients With a Fontan Circulation.
2018,
Pubmed
SHARMA,
The circulatory mechanism and anatomy of the heart of the frog, Rana pipiens.
1961,
Pubmed
Smith,
Comparison of Etomidate, Benzocaine, and MS222 Anesthesia with and without Subsequent Flunixin Meglumine Analgesia in African Clawed Frogs (Xenopus laevis).
2018,
Pubmed
,
Xenbase
