Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
???displayArticle.abstract???
The Xenopus embryo provides an advantageous model system where genes can be readily transplanted as DNA or mRNA or depleted with antisense techniques. Here, we present a protocol to culture and image the cell biological properties of explanted Xenopus cap cells in tissue culture. We illustrate how this protocol can be applied to visualize lysosomes, macropinocytosis, focal adhesions, Wnt signaling, and cell migration. For complete details on the use and execution of this protocol, please refer to Tejeda-Muñoz et al. (2022).
Albrecht,
GSK3 Inhibits Macropinocytosis and Lysosomal Activity through the Wnt Destruction Complex Machinery.
2020, Pubmed,
Xenbase
Albrecht,
GSK3 Inhibits Macropinocytosis and Lysosomal Activity through the Wnt Destruction Complex Machinery.
2020,
Pubmed
,
Xenbase
Albrecht,
Cell Biology of Canonical Wnt Signaling.
2021,
Pubmed
Ariizumi,
Isolation and differentiation of Xenopus animal cap cells.
2009,
Pubmed
,
Xenbase
Asashima,
In vitro organogenesis from undifferentiated cells in Xenopus.
2009,
Pubmed
,
Xenbase
Bachir,
Actin-Based Adhesion Modules Mediate Cell Interactions with the Extracellular Matrix and Neighboring Cells.
2017,
Pubmed
Blum,
Morpholinos: Antisense and Sensibility.
2015,
Pubmed
,
Xenbase
Colozza,
Wnt-inducible Lrp6-APEX2 interacting proteins identify ESCRT machinery and Trk-fused gene as components of the Wnt signaling pathway.
2020,
Pubmed
,
Xenbase
Commisso,
Determining the macropinocytic index of cells through a quantitative image-based assay.
2014,
Pubmed
Galluzzi,
WNT Signaling in Cancer Immunosurveillance.
2019,
Pubmed
Holland,
SnapShot: Lysosomal Functions.
2020,
Pubmed
Lawrence,
The lysosome as a cellular centre for signalling, metabolism and quality control.
2019,
Pubmed
MacDonald,
Wnt/beta-catenin signaling: components, mechanisms, and diseases.
2009,
Pubmed
,
Xenbase
Marciniszyn,
Mode of inhibition of acid proteases by pepstatin.
1976,
Pubmed
Moriyama,
Embryonic regeneration by relocalization of the Spemann organizer during twinning in Xenopus.
2018,
Pubmed
,
Xenbase
Nakatsuji,
Presumptive mesoderm cells from Xenopus laevis gastrulae attach to and migrate on substrata coated with fibronectin or laminin.
1986,
Pubmed
,
Xenbase
Nusse,
Wnt/β-Catenin Signaling, Disease, and Emerging Therapeutic Modalities.
2017,
Pubmed
Redelman-Sidi,
The Canonical Wnt Pathway Drives Macropinocytosis in Cancer.
2018,
Pubmed
Smith,
Mesoderm induction and the control of gastrulation in Xenopus laevis: the roles of fibronectin and integrins.
1990,
Pubmed
,
Xenbase
Smith,
Xenopus cell lines.
1991,
Pubmed
,
Xenbase
Sosa,
Transcriptome analysis of regeneration during Xenopus laevis experimental twinning.
2019,
Pubmed
,
Xenbase
Taelman,
Wnt signaling requires sequestration of glycogen synthase kinase 3 inside multivesicular endosomes.
2010,
Pubmed
,
Xenbase
Tejeda-Muñoz,
Glycogen synthase kinase 3 in Wnt signaling pathway and cancer.
2015,
Pubmed
Tejeda-Muñoz,
Lysosomes are required for early dorsal signaling in the Xenopus embryo.
2022,
Pubmed
,
Xenbase
Tejeda-Muñoz,
Wnt canonical pathway activates macropinocytosis and lysosomal degradation of extracellular proteins.
2019,
Pubmed
Tejeda-Muñoz,
Targeting Membrane Trafficking as a Strategy for Cancer Treatment.
2022,
Pubmed
,
Xenbase
Tejeda-Muñoz,
Canonical Wnt signaling induces focal adhesion and Integrin beta-1 endocytosis.
2022,
Pubmed
,
Xenbase
