Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
???displayArticle.abstract???
Cold-inducible RNA-binding protein (CIRP) was originally found in mammalian cells as a protein that is overexpressed upon a temperature downshift. Recently, we identified a Xenopus homolog of CIRP, termed xCIRP2, as a major cytoplasmic RNA-binding protein in oocytes. In this study we found by yeast two-hybrid screening that the Xenopus homolog of protein arginine N-methyltransferase 1 (xPRMT1) interacted with xCIRP2. We found that an arginine- and glycine-rich region of xCIRP2, termed the RG4 domain, was a target of xPRMT1 for methylation in vitro. xCIRP2 expressed in cultured cells accumulated in the nucleus as does mammalian CIRP. Interestingly, the RG4 domain was necessary for nuclear localization of xCIRP2. RG4-mediated nuclear accumulation of xCIRP2 was diminished in the presence of transcription inhibitors, suggesting that nuclear localization of xCIRP2 was dependent on ongoing transcription with RNA polymerase II. Analysis of interspecies heterokaryons revealed that xCIRP2 was capable of nucleocytoplasmic shuttling and the RG4 domain functioned as a nucleocytoplasmic shuttling signal. Methylation by overexpressed xPRMT1 caused cytoplasmic accumulation of xCIRP2. Possible implications of the relationship between regulation of intracellular localization and multiple functions of xCIRP2 will be discussed.
Abramovich,
A protein-arginine methyltransferase binds to the intracytoplasmic domain of the IFNAR1 chain in the type I interferon receptor.
1997, Pubmed
Abramovich,
A protein-arginine methyltransferase binds to the intracytoplasmic domain of the IFNAR1 chain in the type I interferon receptor.
1997,
Pubmed
Bartel,
Effects of adenosine dialdehyde on S-adenosylhomocysteine hydrolase and S-adenosylmethionine-dependent transmethylations in mouse L929 cells.
1984,
Pubmed
Bhatia,
Expression of axolotl RNA-binding protein during development of the Mexican axolotl.
1999,
Pubmed
,
Xenbase
Boffa,
Distribution of NG, NG,-dimethylarginine in nuclear protein fractions.
1977,
Pubmed
Bouvet,
Nucleolin interacts with several ribosomal proteins through its RGG domain.
1998,
Pubmed
Burd,
Conserved structures and diversity of functions of RNA-binding proteins.
1994,
Pubmed
Cáceres,
A specific subset of SR proteins shuttles continuously between the nucleus and the cytoplasm.
1998,
Pubmed
Daneholt,
A look at messenger RNP moving through the nuclear pore.
1997,
Pubmed
Dreyfuss,
hnRNP proteins and the biogenesis of mRNA.
1993,
Pubmed
Egyházi,
5,6-Dichlororibofuranosylbenzimidazole inhibits the rate of transcription initiation in intact Chironomus cells.
1982,
Pubmed
Fan,
HNS, a nuclear-cytoplasmic shuttling sequence in HuR.
1998,
Pubmed
,
Xenbase
Feldmann,
Complete DNA sequence of yeast chromosome II.
1994,
Pubmed
Friesen,
SMN, the product of the spinal muscular atrophy gene, binds preferentially to dimethylarginine-containing protein targets.
2001,
Pubmed
Gama-Carvalho,
Nucleocytoplasmic shuttling of heterodimeric splicing factor U2AF.
2001,
Pubmed
Gary,
The predominant protein-arginine methyltransferase from Saccharomyces cerevisiae.
1996,
Pubmed
Goffeau,
Life with 6000 genes.
1996,
Pubmed
Görlich,
Transport between the cell nucleus and the cytoplasm.
1999,
Pubmed
Green,
Nab2p is required for poly(A) RNA export in Saccharomyces cerevisiae and is regulated by arginine methylation via Hmt1p.
2002,
Pubmed
Henry,
A novel methyltransferase (Hmt1p) modifies poly(A)+-RNA-binding proteins.
1996,
Pubmed
Kataoka,
Transportin-SR, a nuclear import receptor for SR proteins.
1999,
Pubmed
Kim,
Identification of N(G)-methylarginine residues in human heterogeneous RNP protein A1: Phe/Gly-Gly-Gly-Arg-Gly-Gly-Gly/Phe is a preferred recognition motif.
1997,
Pubmed
Kumar,
Purification and domain structure of core hnRNP proteins A1 and A2 and their relationship to single-stranded DNA-binding proteins.
1986,
Pubmed
Lee,
PABP1 identified as an arginine methyltransferase substrate using high-density protein arrays.
2002,
Pubmed
Liteplo,
DNA (cytosine) methylation in murine and human tumor cell lines treated with S-adenosylhomocysteine hydrolase inhibitors.
1988,
Pubmed
Liu,
A Crm1p-independent nuclear export path for the mRNA-associated protein, Npl3p/Mtr13p.
1999,
Pubmed
Liu,
In vivo and in vitro arginine methylation of RNA-binding proteins.
1995,
Pubmed
Matsumoto,
CIRP2, a major cytoplasmic RNA-binding protein in Xenopus oocytes.
2000,
Pubmed
,
Xenbase
Matsumoto,
Sperm chromatin decondensation by template activating factor I through direct interaction with basic proteins.
1999,
Pubmed
,
Xenbase
McBride,
State of the arg: protein methylation at arginine comes of age.
2001,
Pubmed
Michael,
A nuclear export signal in hnRNP A1: a signal-mediated, temperature-dependent nuclear protein export pathway.
1995,
Pubmed
Michael,
Nucleocytoplasmic shuttling signals: two for the price of one.
2000,
Pubmed
Michael,
The K nuclear shuttling domain: a novel signal for nuclear import and nuclear export in the hnRNP K protein.
1997,
Pubmed
,
Xenbase
Moore,
Nuclear RNA turnover.
2002,
Pubmed
Moser,
Mouse-human heterokaryon analysis with a 33258 Hoechst-Giemsa technique.
1975,
Pubmed
Mowen,
Arginine methylation of STAT1 modulates IFNalpha/beta-induced transcription.
2001,
Pubmed
Nakielny,
Transport of proteins and RNAs in and out of the nucleus.
1999,
Pubmed
Nishiyama,
Cloning and characterization of human CIRP (cold-inducible RNA-binding protein) cDNA and chromosomal assignment of the gene.
1997,
Pubmed
Nishiyama,
A glycine-rich RNA-binding protein mediating cold-inducible suppression of mammalian cell growth.
1997,
Pubmed
Nishiyama,
Decreased expression of cold-inducible RNA-binding protein (CIRP) in male germ cells at elevated temperature.
1998,
Pubmed
Niwa,
Efficient selection for high-expression transfectants with a novel eukaryotic vector.
1991,
Pubmed
Peng,
Maternal cold inducible RNA binding protein is required for embryonic kidney formation in Xenopus laevis.
2000,
Pubmed
,
Xenbase
Piñol-Roma,
Shuttling of pre-mRNA binding proteins between nucleus and cytoplasm.
1992,
Pubmed
,
Xenbase
Pollard,
A novel receptor-mediated nuclear protein import pathway.
1996,
Pubmed
Rajpurohit,
Effect of enzymic methylation of heterogeneous ribonucleoprotein particle A1 on its nucleic-acid binding and controlled proteolysis.
1994,
Pubmed
Rajpurohit,
Enzymatic methylation of recombinant heterogeneous nuclear RNP protein A1. Dual substrate specificity for S-adenosylmethionine:histone-arginine N-methyltransferase.
1994,
Pubmed
Raman,
N(omega)-arginine dimethylation modulates the interaction between a Gly/Arg-rich peptide from human nucleolin and nucleic acids.
2001,
Pubmed
Reed,
A conserved mRNA export machinery coupled to pre-mRNA splicing.
2002,
Pubmed
Saito,
Cloning and characterization of amphibian cold inducible RNA-binding protein.
2000,
Pubmed
,
Xenbase
Scott,
Identification and characterization of two putative human arginine methyltransferases (HRMT1L1 and HRMT1L2).
1998,
Pubmed
Sheikh,
Identification of several human homologs of hamster DNA damage-inducible transcripts. Cloning and characterization of a novel UV-inducible cDNA that codes for a putative RNA-binding protein.
1997,
Pubmed
Shen,
Arginine methylation facilitates the nuclear export of hnRNP proteins.
1998,
Pubmed
Shyu,
The double lives of shuttling mRNA binding proteins.
2000,
Pubmed
Siomi,
RNA-binding proteins as regulators of gene expression.
1997,
Pubmed
Siomi,
A nuclear localization domain in the hnRNP A1 protein.
1995,
Pubmed
Smith,
Unusual sites of arginine methylation in Poly(A)-binding protein II and in vitro methylation by protein arginine methyltransferases PRMT1 and PRMT3.
1999,
Pubmed
Stutz,
Nuclear RNA export.
1998,
Pubmed
Tang,
PRMT1 is the predominant type I protein arginine methyltransferase in mammalian cells.
2000,
Pubmed
Tang,
PRMT 3, a type I protein arginine N-methyltransferase that differs from PRMT1 in its oligomerization, subcellular localization, substrate specificity, and regulation.
1998,
Pubmed
Truant,
Identification and functional characterization of a novel nuclear localization signal present in the yeast Nab2 poly(A)+ RNA binding protein.
1998,
Pubmed
Uochi,
XCIRP (Xenopus homolog of cold-inducible RNA-binding protein) is expressed transiently in developing pronephros and neural tissue.
1998,
Pubmed
,
Xenbase
Valentini,
Arginine methylation and binding of Hrp1p to the efficiency element for mRNA 3'-end formation.
1999,
Pubmed
Wang,
Methylation of histone H4 at arginine 3 facilitating transcriptional activation by nuclear hormone receptor.
2001,
Pubmed
,
Xenbase
Weighardt,
Nucleo-cytoplasmic distribution of human hnRNP proteins: a search for the targeting domains in hnRNP A1.
1995,
Pubmed
Xue,
Effects of ischemia and H2O2 on the cold stress protein CIRP expression in rat neuronal cells.
1999,
Pubmed
Yang,
The UV-inducible RNA-binding protein A18 (A18 hnRNP) plays a protective role in the genotoxic stress response.
2001,
Pubmed
Yun,
Conserved SR protein kinase functions in nuclear import and its action is counteracted by arginine methylation in Saccharomyces cerevisiae.
2000,
Pubmed
