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EMBO Rep
2002 Dec 01;312:1209-14. doi: 10.1093/embo-reports/kvf241.
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The D-Box-activating domain (DAD) is a new proteolysis signal that stimulates the silent D-Box sequence of Aurora-A.
Castro A
,
Vigneron S
,
Bernis C
,
Labbé JC
,
Prigent C
,
Lorca T
.
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We have demonstrated previously that Xenopus Aurora-A is degraded at late mitosis by the APC/Fizzy-Related in a D-Box-dependent manner. Here we demonstrate that, although Aurora-B possesses the same D-Box as Aurora-A, Aurora-B is not degraded by this ubiquitin ligase. We have constructed a chimera Aurora-A/B with the N-terminus of Aurora-A and the C-terminus of Aurora-B and we have examined its degradation by APC/Fizzy-Related. We demonstrate that the N-terminus of Aurora-A confers degradation capacity on the C-terminus of Aurora-B and that this feature is blocked by mutation of the conserved D-Box sequence. We characterize the minimal degradation signal at the N-terminus of Aurora-A and demonstrate that its deletion blocks the degradation of this protein by APC/Fizzy-Related. Thus, we conclude that two different degradation signals are required for proteolysis of Aurora-A. The first one, which we designated D-Box-activating domain, within the N-terminal domain of Aurora-A confers the functionality to the second, a silent D-Box, present within the C-terminus of the kinase.
Bischoff,
A homologue of Drosophila aurora kinase is oncogenic and amplified in human colorectal cancers.
1998, Pubmed
Bischoff,
A homologue of Drosophila aurora kinase is oncogenic and amplified in human colorectal cancers.
1998,
Pubmed
Castro,
APC/Fizzy-Related targets Aurora-A kinase for proteolysis.
2002,
Pubmed
,
Xenbase
Castro,
Cyclin B/cdc2 induces c-Mos stability by direct phosphorylation in Xenopus oocytes.
2001,
Pubmed
,
Xenbase
Cohen-Fix,
Anaphase initiation in Saccharomyces cerevisiae is controlled by the APC-dependent degradation of the anaphase inhibitor Pds1p.
1996,
Pubmed
,
Xenbase
Crosio,
Mitotic phosphorylation of histone H3: spatio-temporal regulation by mammalian Aurora kinases.
2002,
Pubmed
Fang,
Direct binding of CDC20 protein family members activates the anaphase-promoting complex in mitosis and G1.
1998,
Pubmed
,
Xenbase
Fesquet,
Is Cdk7/cyclin H/MAT1 the genuine cdk activating kinase in cycling Xenopus egg extracts?
1997,
Pubmed
,
Xenbase
Giet,
Drosophila Aurora A kinase is required to localize D-TACC to centrosomes and to regulate astral microtubules.
2002,
Pubmed
,
Xenbase
Giet,
Aurora/Ipl1p-related kinases, a new oncogenic family of mitotic serine-threonine kinases.
1999,
Pubmed
Giet,
The Xenopus laevis aurora/Ip11p-related kinase pEg2 participates in the stability of the bipolar mitotic spindle.
2000,
Pubmed
,
Xenbase
Glotzer,
Cyclin is degraded by the ubiquitin pathway.
1991,
Pubmed
,
Xenbase
Hagting,
Human securin proteolysis is controlled by the spindle checkpoint and reveals when the APC/C switches from activation by Cdc20 to Cdh1.
2002,
Pubmed
Honda,
Degradation of human Aurora2 protein kinase by the anaphase-promoting complex-ubiquitin-proteasome pathway.
2000,
Pubmed
Kimura,
Cell cycle-dependent expression and spindle pole localization of a novel human protein kinase, Aik, related to Aurora of Drosophila and yeast Ipl1.
1997,
Pubmed
,
Xenbase
Kimura,
Cell cycle-dependent expression and centrosome localization of a third human aurora/Ipl1-related protein kinase, AIK3.
1999,
Pubmed
,
Xenbase
King,
Mutagenic analysis of the destruction signal of mitotic cyclins and structural characterization of ubiquitinated intermediates.
1996,
Pubmed
,
Xenbase
Kramer,
Mitotic regulation of the APC activator proteins CDC20 and CDH1.
2000,
Pubmed
,
Xenbase
Littlepage,
Identification of a new APC/C recognition domain, the A box, which is required for the Cdh1-dependent destruction of the kinase Aurora-A during mitotic exit.
2002,
Pubmed
,
Xenbase
Lorca,
Fizzy is required for activation of the APC/cyclosome in Xenopus egg extracts.
1998,
Pubmed
,
Xenbase
Nigg,
Mitotic kinases as regulators of cell division and its checkpoints.
2001,
Pubmed
Pfleger,
The KEN box: an APC recognition signal distinct from the D box targeted by Cdh1.
2000,
Pubmed
,
Xenbase
Pogulis,
In vitro recombination and mutagenesis by overlap extension PCR.
1996,
Pubmed
Raff,
The roles of Fzy/Cdc20 and Fzr/Cdh1 in regulating the destruction of cyclin B in space and time.
2002,
Pubmed
Roghi,
The Xenopus protein kinase pEg2 associates with the centrosome in a cell cycle-dependent manner, binds to the spindle microtubules and is involved in bipolar mitotic spindle assembly.
1998,
Pubmed
,
Xenbase
Shannon,
Chromosome dynamics: new light on Aurora B kinase function.
2002,
Pubmed
Shirayama,
The Polo-like kinase Cdc5p and the WD-repeat protein Cdc20p/fizzy are regulators and substrates of the anaphase promoting complex in Saccharomyces cerevisiae.
1998,
Pubmed
Sigrist,
Drosophila fizzy-related down-regulates mitotic cyclins and is required for cell proliferation arrest and entry into endocycles.
1997,
Pubmed
,
Xenbase
Uzbekov,
Cell cycle analysis and synchronization of the Xenopus cell line XL2.
1998,
Pubmed
,
Xenbase
Visintin,
CDC20 and CDH1: a family of substrate-specific activators of APC-dependent proteolysis.
1997,
Pubmed
Zachariae,
Whose end is destruction: cell division and the anaphase-promoting complex.
1999,
Pubmed
Zhou,
Tumour amplified kinase STK15/BTAK induces centrosome amplification, aneuploidy and transformation.
1998,
Pubmed
