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c-MOS, a MAP kinase kinase kinase, is a regulator of oocyte maturation. The concentration of c-MOS is controlled in part through its conditional degradation. Previous studies proposed the "second-codon rule", according to which the N-terminal proline (Pro) of c-MOS is a destabilizing residue that targets c-MOS for degradation. We analyzed the degradation signal (degron) of c-MOS in Xenopus oocytes, found it to be a portable degron, and demonstrated that, contrary to the model above, the N-terminal Pro residue of c-MOS is entirely dispensable for its degradation if Ser-2 (encoded Ser-3) of c-MOS is replaced by a small non-phosphorylatable residue such as Gly. The dependence of c-MOS degradation on N-terminal Pro is shown to be caused by a Pro-mediated downregulation of the net phosphorylation of Ser-2, a modification that halts c-MOS degradation in oocytes. Thus, the N-terminal Pro residue of c-MOS is not a recognition determinant for a ubiquitin ligase, in agreement with earlier evidence that Pro is a stabilizing residue in the N-end rule.
Bachmair,
The degradation signal in a short-lived protein.
1989, Pubmed
Bachmair,
The degradation signal in a short-lived protein.
1989,
Pubmed
Bachmair,
In vivo half-life of a protein is a function of its amino-terminal residue.
1986,
Pubmed
Bradshaw,
N-terminal processing: the methionine aminopeptidase and N alpha-acetyl transferase families.
1998,
Pubmed
Castro,
Cyclin B/cdc2 induces c-Mos stability by direct phosphorylation in Xenopus oocytes.
2001,
Pubmed
,
Xenbase
Charlesworth,
A novel regulatory element determines the timing of Mos mRNA translation during Xenopus oocyte maturation.
2002,
Pubmed
,
Xenbase
Davydov,
RGS4 is arginylated and degraded by the N-end rule pathway in vitro.
2000,
Pubmed
de Groot,
Sindbis virus RNA polymerase is degraded by the N-end rule pathway.
1991,
Pubmed
Duesbery,
Developmental biology: an arresting activity.
2002,
Pubmed
,
Xenbase
Dupré,
Mos is not required for the initiation of meiotic maturation in Xenopus oocytes.
2002,
Pubmed
,
Xenbase
Ferrell,
Xenopus oocyte maturation: new lessons from a good egg.
1999,
Pubmed
,
Xenbase
Ferrell,
Self-perpetuating states in signal transduction: positive feedback, double-negative feedback and bistability.
2002,
Pubmed
,
Xenbase
Freeman,
Phosphorylation of conserved serine residues does not regulate the ability of mosxe protein kinase to induce oocyte maturation or function as cytostatic factor.
1992,
Pubmed
,
Xenbase
Gonda,
Universality and structure of the N-end rule.
1989,
Pubmed
Gurdon,
The use of Xenopus oocytes for the expression of cloned genes.
1983,
Pubmed
,
Xenbase
Hochegger,
New B-type cyclin synthesis is required between meiosis I and II during Xenopus oocyte maturation.
2001,
Pubmed
,
Xenbase
Kwon,
An essential role of N-terminal arginylation in cardiovascular development.
2002,
Pubmed
Kwon,
Construction and analysis of mouse strains lacking the ubiquitin ligase UBR1 (E3alpha) of the N-end rule pathway.
2001,
Pubmed
Mendez,
Phosphorylation of CPE binding factor by Eg2 regulates translation of c-mos mRNA.
2000,
Pubmed
,
Xenbase
Murray,
Cell cycle extracts.
1991,
Pubmed
Nebreda,
Regulation of the meiotic cell cycle in oocytes.
2000,
Pubmed
,
Xenbase
Nishizawa,
Degradation of Mos by the N-terminal proline (Pro2)-dependent ubiquitin pathway on fertilization of Xenopus eggs: possible significance of natural selection for Pro2 in Mos.
1993,
Pubmed
,
Xenbase
Nishizawa,
The 'second-codon rule' and autophosphorylation govern the stability and activity of Mos during the meiotic cell cycle in Xenopus oocytes.
1992,
Pubmed
,
Xenbase
Pham,
Evidence for an important role of serine 16 and its phosphorylation in the stabilization of c-Mos.
1999,
Pubmed
Pudney,
Establishment of a cell line (XTC-2) from the South African clawed toad, Xenopus laevis.
1973,
Pubmed
,
Xenbase
Rao,
Degradation of a cohesin subunit by the N-end rule pathway is essential for chromosome stability.
2001,
Pubmed
Reimann,
Emi1 is required for cytostatic factor arrest in vertebrate eggs.
2002,
Pubmed
,
Xenbase
Sagata,
Function of c-mos proto-oncogene product in meiotic maturation in Xenopus oocytes.
1988,
Pubmed
,
Xenbase
Sagata,
What does Mos do in oocytes and somatic cells?
1997,
Pubmed
,
Xenbase
Suzuki,
Degradation signals in the lysine-asparagine sequence space.
1999,
Pubmed
Turner,
Detecting and measuring cotranslational protein degradation in vivo.
2000,
Pubmed
Turner,
Peptides accelerate their uptake by activating a ubiquitin-dependent proteolytic pathway.
2000,
Pubmed
Varshavsky,
The N-end rule: functions, mysteries, uses.
1996,
Pubmed
Varshavsky,
Ubiquitin fusion technique and its descendants.
2000,
Pubmed
