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We used immunofluorescence to study the distribution and targeting of RNA polymerase (pol) III subunits and pol III transcription factors in the Xenopus laevis oocytenucleus. Antibodies against several of these proteins stained Cajal bodies and approximately 90 specific sites on the lampbrush chromosomes. Some of the chromosomal sites had been identified previously by in situ hybridization as the genes for 5S rRNA. The remaining sites presumably encode tRNAs and other pol III transcripts. Pol III sites were often resolvable as loops similar to the much more abundant pol II loops, but without a matrix detectable by phase contrast or differential interference contrast. This morphology is consistent with the transcription of short repeated sequences. Hemagglutinin-tagged transcripts encoding core subunits and transcription factors were injected into the oocytecytoplasm, and the distribution of newly translated proteins inside the nucleus was monitored by immunostaining. Cajal bodies were preferentially targeted by these proteins, and in some cases the chromosomal sites were also weakly stained. The existence of pol III subunits and pol III transcription factors in Cajal bodies and their targeting to these organelles are consistent with a model of Cajal bodies as sites for preassembly of the nuclear transcription machinery.
Acker,
A 14.4 KDa acidic subunit of human RNA polymerase II with a putative leucine-zipper.
1994, Pubmed
Acker,
A 14.4 KDa acidic subunit of human RNA polymerase II with a putative leucine-zipper.
1994,
Pubmed
Angelier,
[Transcription complexes with nucleolar and chromosomal origins in oocytes of Pleurodeles waltlii and P. poireti (amphibia, urodela) (author's transl)].
1975,
Pubmed
Brown,
5 S DNAs of Xenopus laevis and Xenopus mulleri: evolution of a gene family.
1973,
Pubmed
,
Xenbase
Brown,
Purification and some characteristics of 5S DNA from Xenopus laevis.
1971,
Pubmed
,
Xenbase
Bucci,
Incorporation of tritiated uridine in nuclei of Triturus oocytes treated with alpha-amanitin.
1971,
Pubmed
Callan,
The lampbrush chromosomes of Xenopus laevis: preparation, identification, and distribution of 5S DNA sequences.
1987,
Pubmed
,
Xenbase
Clarkson,
Clustering of transfer RNA genes of Xenopus laevis.
1973,
Pubmed
,
Xenbase
Clarkson,
Reiterated transfer RNA genes of Xenopus laevis.
1973,
Pubmed
,
Xenbase
Clarkson,
Isolation and some properties of DNA coding for tRNA1met from Xenopus laevis.
1976,
Pubmed
,
Xenbase
Evan,
Isolation of monoclonal antibodies specific for human c-myc proto-oncogene product.
1985,
Pubmed
,
Xenbase
Fostel,
Chromosomal location of a major tRNA gene cluster of Xenopus laevis.
1984,
Pubmed
,
Xenbase
Gall,
Assembly of the nuclear transcription and processing machinery: Cajal bodies (coiled bodies) and transcriptosomes.
1999,
Pubmed
,
Xenbase
Gall,
Cajal bodies: the first 100 years.
2000,
Pubmed
Gall,
A role for Cajal bodies in assembly of the nuclear transcription machinery.
2001,
Pubmed
Hsieh,
Cloning and characterization of two evolutionarily conserved subunits (TFIIIC102 and TFIIIC63) of human TFIIIC and their involvement in functional interactions with TFIIIB and RNA polymerase III.
1999,
Pubmed
Huang,
Comparison of the RNA polymerase III transcription machinery in Schizosaccharomyces pombe, Saccharomyces cerevisiae and human.
2001,
Pubmed
Hügle,
Ribocharin: a nuclear Mr 40,000 protein specific to precursor particles of the large ribosomal subunit.
1985,
Pubmed
,
Xenbase
Ittmann,
Cell cycle control of the BN51 cell cycle gene which encodes a subunit of RNA polymerase III.
1994,
Pubmed
Kozak,
The scanning model for translation: an update.
1989,
Pubmed
Krol,
Xenopus tropicalis U6 snRNA genes transcribed by Pol III contain the upstream promoter elements used by Pol II dependent U snRNA genes.
1987,
Pubmed
,
Xenbase
Laemmli,
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
1970,
Pubmed
Mancino,
Effects of alpha-amanitin on Triturus lampbrush chromosomes.
1971,
Pubmed
Miller,
Visualization of RNA synthesis on chromosomes.
1972,
Pubmed
Morgan,
RNA polymerase II in Cajal bodies of amphibian oocytes.
2000,
Pubmed
,
Xenbase
Müller,
Sequence of a 3.18 kb tandem repeat of Xenopus laevis DNA containing 8 tRNA genes.
1987,
Pubmed
,
Xenbase
Narayanswami,
Chromosomal locations of major tRNA gene clusters of Xenopus laevis.
1995,
Pubmed
,
Xenbase
Pardue,
Location of the genes for 5S ribosomal RNA in Xenopus laevis.
1973,
Pubmed
,
Xenbase
Paule,
Survey and summary: transcription by RNA polymerases I and III.
2000,
Pubmed
Roth,
A conserved family of nuclear phosphoproteins localized to sites of polymerase II transcription.
1991,
Pubmed
,
Xenbase
Scheer,
Classification of loops of lampbrush chromosomes according to the arrangement of transcriptional complexes.
1976,
Pubmed
Verheggen,
Mammalian and yeast U3 snoRNPs are matured in specific and related nuclear compartments.
2002,
Pubmed
,
Xenbase
Verheggen,
Box C/D small nucleolar RNA trafficking involves small nucleolar RNP proteins, nucleolar factors and a novel nuclear domain.
2001,
Pubmed
Wallace,
Protein incorporation by isolated amphibian oocytes. 3. Optimum incubation conditions.
1973,
Pubmed
,
Xenbase
Wang,
Three human RNA polymerase III-specific subunits form a subcomplex with a selective function in specific transcription initiation.
1997,
Pubmed
Wang,
Structure and function of a human transcription factor TFIIIB subunit that is evolutionarily conserved and contains both TFIIB- and high-mobility-group protein 2-related domains.
1995,
Pubmed
Wu,
Human p80-coilin is targeted to sphere organelles in the amphibian germinal vesicle.
1994,
Pubmed
,
Xenbase
