Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
???displayArticle.abstract???
Popeye genes code for putative transmembrane proteins that are predominantly expressed in heart and skeletal muscle. Here we report on the isolation and expression of a previously unknown Xenopus member of this family, Xenopus Popeye-1 (Xpop-1). Xpop-1 is 60-65% identical to other vertebrate Pop-1 genes at the protein level. Whole-mount in situ hybridization studies revealed a highly specific expression of Xpop-1 whose transcripts are restricted to the embryonic heart and become enriched in the forming ventricle. Interestingly, unlike other known vertebrate Popeye genes, Xpop-1 is exclusively expressed in cardiac tissue and absent from skeletal muscle.
???displayArticle.pubmedLink???
12049775
???displayArticle.link???Mech Dev
Fig. 2. Temporal and spatial expression of Xenopus Pop-1. (A) RT-PCR analyses of embryonic Xenopus Pop-1 expression during different developmental stages as indicated. RT-PCR was performed using total RNA isolated from embryos which were cultured until the indicated developmental stages. The temporal expression of Xpop-1 was compared to the developmental expression profile of other cardiac marker genes. Abbreviations used: CA, cardiac actin; MHC, myosin heavy chain α; ANF, atrial natriuretic factor; H4, histone 4. The RT-reaction was performed with H4 primers. (B) Expression of Xenopus Pop-1 in different adult tissues as indicated. RT-PCR was performed using total RNA isolated from the indicated adult tissues. Xpop-1 transcripts were only detected in the adult heart. (C) Spatial expression of Xpop-1 and Nkx2.5 at stage 30. Transverse sections demonstrate the specific expression of Xpop-1 in the myocardium (mc). Nkx2.5 transcripts are abundant in the myocardium but in contrast to Xpop-1, Nkx2.5 is additionally, albeit weaker, expressed in tissue fated to become the dorsal roof of the pericardium (pc) and in the pharyngeal pouches (arrow heads). (D) Expression pattern of Xpop-1 in the developing heart chambers at stages 33, 36, and 41. Abbreviations used: a, atrium; v, ventricle; o, outflow tract.
bves (blood vessel epicardial substance) gene expression in Xenopus laevis embryos, NF stage 33, as assayed by in situ hybridization. Lateral view: anteriorleft, dorsal up; also, transverse section of same at the plane of the heart
