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Proc Natl Acad Sci U S A
2002 Apr 02;997:4361-6. doi: 10.1073/pnas.022056399.
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Inhibition of Xenopus oocyte meiotic maturation by catalytically inactive protein kinase A.
Schmitt A
,
Nebreda AR
.
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Progesterone induces G2-arrested Xenopus oocytes to develop into fertilizable eggs in a process called meiotic maturation. Protein kinase A (PKA), the cAMP-dependent protein kinase, has long been known to be a potent inhibitor of meiotic maturation, but little information is available on how PKA functions. We have cloned two Xenopus PKA catalytic subunit isoforms, XPKAalpha and XPKAbeta. These proteins are 89% identical and both inhibit progesterone-induced meiotic maturation when overexpressed at low levels, suggesting that PKA activity is tightly regulated in the oocyte. Unexpectedly, catalytically inactive XPKA mutants are able to block progesterone-induced maturation as efficiently as the wild-type active XPKA. These mutants also block meiotic maturation induced by Mos, but are less efficient at inhibiting Cdc25C-induced maturation. Our results indicate that PKA can inhibit meiotic maturation by a novel mechanism, which does not require its kinase activity and is also independent of binding to the PKA regulatory subunits.
Andersen,
Protein kinase B/Akt induces resumption of meiosis in Xenopus oocytes.
1998, Pubmed,
Xenbase
Andersen,
Protein kinase B/Akt induces resumption of meiosis in Xenopus oocytes.
1998,
Pubmed
,
Xenbase
Bardwell,
Repression of yeast Ste12 transcription factor by direct binding of unphosphorylated Kss1 MAPK and its regulation by the Ste7 MEK.
1998,
Pubmed
Beebe,
The C gamma subunit is a unique isozyme of the cAMP-dependent protein kinase.
1992,
Pubmed
Beebe,
The cAMP-dependent protein kinases and cAMP signal transduction.
1994,
Pubmed
Biondi,
Identification of a pocket in the PDK1 kinase domain that interacts with PIF and the C-terminal residues of PKA.
2000,
Pubmed
Cheng,
Phosphorylation and activation of cAMP-dependent protein kinase by phosphoinositide-dependent protein kinase.
1998,
Pubmed
Cicirelli,
Activation of multiple protein kinases during the burst in protein phosphorylation that precedes the first meiotic cell division in Xenopus oocytes.
1988,
Pubmed
,
Xenbase
Cox,
Holoenzyme interaction sites in the cAMP-dependent protein kinase. Histidine 87 in the catalytic subunit complements serine 99 in the type I regulatory subunit.
1994,
Pubmed
Daar,
Inhibition of mos-induced oocyte maturation by protein kinase A.
1993,
Pubmed
,
Xenbase
Faure,
Inactivation of protein kinase A is not required for c-mos translation during meiotic maturation of Xenopus oocytes.
1998,
Pubmed
,
Xenbase
Ferby,
A novel p34(cdc2)-binding and activating protein that is necessary and sufficient to trigger G(2)/M progression in Xenopus oocytes.
1999,
Pubmed
,
Xenbase
Ferrell,
Xenopus oocyte maturation: new lessons from a good egg.
1999,
Pubmed
,
Xenbase
Gamm,
The major catalytic subunit isoforms of cAMP-dependent protein kinase have distinct biochemical properties in vitro and in vivo.
1996,
Pubmed
Gavin,
A p90(rsk) mutant constitutively interacting with MAP kinase uncouples MAP kinase from p34(cdc2)/cyclin B activation in Xenopus oocytes.
1999,
Pubmed
,
Xenbase
Gibbs,
Rational scanning mutagenesis of a protein kinase identifies functional regions involved in catalysis and substrate interactions.
1991,
Pubmed
Gibson,
Dissecting the cooperative reassociation of the regulatory and catalytic subunits of cAMP-dependent protein kinase. Role of Trp-196 in the catalytic subunit.
1997,
Pubmed
Grieco,
A role for cAMP-dependent protein kinase in early embryonic divisions.
1994,
Pubmed
,
Xenbase
Howard,
The mitogen-activated protein kinase signaling pathway stimulates mos mRNA cytoplasmic polyadenylation during Xenopus oocyte maturation.
1999,
Pubmed
,
Xenbase
Karaïskou,
Phosphatase 2A and polo kinase, two antagonistic regulators of cdc25 activation and MPF auto-amplification.
1999,
Pubmed
,
Xenbase
Lawlor,
PKB/Akt: a key mediator of cell proliferation, survival and insulin responses?
2001,
Pubmed
Madhani,
MAP kinases with distinct inhibitory functions impart signaling specificity during yeast differentiation.
1997,
Pubmed
Maller,
Progesterone-stimulated meiotic cell division in Xenopus oocytes. Induction by regulatory subunit and inhibition by catalytic subunit of adenosine 3':5'-monophosphate-dependent protein kinase.
1977,
Pubmed
,
Xenbase
Maller,
Regulation of oocyte maturation.
1980,
Pubmed
,
Xenbase
Masaracchia,
Histone 1 phosphotransferase activities during the maturation of oocytes of Xenopus laevis.
1979,
Pubmed
,
Xenbase
Matten,
Positive feedback between MAP kinase and Mos during Xenopus oocyte maturation.
1996,
Pubmed
,
Xenbase
Matten,
Protein kinase A acts at multiple points to inhibit Xenopus oocyte maturation.
1994,
Pubmed
,
Xenbase
Mulner,
Cyclic AMP synthesis in Xenopus laevis oocytes: inhibition by progesterone.
1979,
Pubmed
,
Xenbase
Nebreda,
Regulation of the meiotic cell cycle in oocytes.
2000,
Pubmed
,
Xenbase
Okumura,
Akt inhibits Myt1 in the signalling pathway that leads to meiotic G2/M-phase transition.
2002,
Pubmed
Olsen,
Isolation and characterization of cDNA clones for an inhibitor protein of cAMP-dependent protein kinase.
1991,
Pubmed
Palmer,
A link between MAP kinase and p34(cdc2)/cyclin B during oocyte maturation: p90(rsk) phosphorylates and inactivates the p34(cdc2) inhibitory kinase Myt1.
1998,
Pubmed
,
Xenbase
Ray,
Catalytic subunit of protein kinase A is an interacting partner of the inflammation-responsive transcription factor serum amyloid A-activating factor-1.
2001,
Pubmed
Rime,
Activation of p34cdc2 kinase by cyclin is negatively regulated by cyclic amp-dependent protein kinase in Xenopus oocytes.
1992,
Pubmed
,
Xenbase
Sadler,
Progesterone inhibits adenylate cyclase in Xenopus oocytes. Action on the guanine nucleotide regulatory protein.
1981,
Pubmed
,
Xenbase
Sharp-Baker,
Spindle checkpoint protein Bub1 is required for kinetochore localization of Mad1, Mad2, Bub3, and CENP-E, independently of its kinase activity.
2001,
Pubmed
,
Xenbase
Slice,
Expression of the catalytic subunit of cAMP-dependent protein kinase in Escherichia coli.
1989,
Pubmed
Stewart,
Kinase suppressor of Ras forms a multiprotein signaling complex and modulates MEK localization.
1999,
Pubmed
Taskén,
Structure, function, and regulation of human cAMP-dependent protein kinases.
1997,
Pubmed
Taylor,
cAMP-dependent protein kinase: framework for a diverse family of regulatory enzymes.
1990,
Pubmed
Taylor,
A template for the protein kinase family.
1993,
Pubmed
Walsh,
Krebs EG: Purification and characterization of a protein inhibitor of adenosine 3',5'-monophosphate-dependent protein kinases.
1971,
Pubmed
Wen,
High affinity binding of the heat-stable protein kinase inhibitor to the catalytic subunit of cAMP-dependent protein kinase is selectively abolished by mutation of Arg133.
1994,
Pubmed
Zhong,
The transcriptional activity of NF-kappaB is regulated by the IkappaB-associated PKAc subunit through a cyclic AMP-independent mechanism.
1997,
Pubmed
Zoller,
Affinity labeling of cAMP-dependent protein kinase with p-fluorosulfonylbenzoyl adenosine. Covalent modification of lysine 71.
1981,
Pubmed
