Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
???displayArticle.abstract???
We have investigated the sequence and expression pattern of the Xenopus laevis FoxD2 gene, a member of the fork head/winged helix multigene family. The derived protein sequence is most closely related to FoxD2 factors known from other species. Maternal FoxD2 transcripts are degraded during early cleavage stages. Zygotic transcription is activated after the midblastula transition followed by a pronounced increase during neurulation. Whole mount in situ hybridisations reveal that FoxD2 is predominantly expressed in the paraxial mesoderm, but not within the myotome. In addition, FoxD2 transcripts are found within the migrating ventral abdominal muscle precursors, in cranial neural crest cells surrounding the eye and populating the second and third visceral arches as well as within restricted areas of the diencephalon. In hatched tadpoles, FoxD2 expression is also observed within the terminal part of the gut.
???displayArticle.pubmedLink???
11804794
???displayArticle.link???Mech Dev
Fig. 1. Sequence of X. laevis FoxD2. (A) Nucleotide and derived amino acid sequences. The sequence has been deposited under EMBL accession number AJ344435. The TATA box, the polyadenylation signal and the fork head/winged helix domain are underlined. (B) Sequence alignment of X. laevis (x) FoxD2 with chicken (g) FoxD2, X. laevis FoxD1 and FoxD3. Identical aa residues are shown in red, deletions are indicated by dashes. (C) Phylogenetic tree for X. laevis FoxD1 (xBF-2; AJ011652), FoxD2, FoxD3 (XFD-6; AJ298865), human FOXD1 (FREAC4; U59831), chicken FoxD2 (CWH1; U37272) and FoxD3 (CWH3; U37274) (DNAstar program using J. Hein method with PAM 250). (D) Comparative whole mount in situ hybridisation for FoxD1, FoxD2 and FoxD3 during (upper; anterior view) or after completion of neurulation (lower; lateral view), respectively.
Fig. 3. Whole mount in situ hybridisation with FoxD2. (A) Stage 11, lateral view. (B) Inner view of an embryo (stage 11) cut into two halves. The insert shows a sagittal section through the dorsal blastopore lip of an embryo after whole mount in situ hybridisation for Xbra and DAPI staining of nuclei. (C) Stage 15. (D) Stage 17. (E) Transverse section of D. (F) Stage 21. (G) Stage 25. (H) Transverse section of G. (I) Stage 27. (J) Stage 29. (K) Transverse section of J. (L) Horizontal section of J. (M) Stage 36. (N) Higher magnification of the lateral region at stage 36. (O) Stage 41. (P) Transverse section of stage 41. All embryos, except for O, are orientated with anterior to the right. (C, D, F, G): dorsal view. (I, J, M, N, O): lateral view. dl: dorsal lip, ey: eye, me: mesencephalon, no: notochord, np: neural plate, nt: neural tube, II. and III.: second and third visceral pouch. Red dotted lines denote the planes of sections.
foxd2 (forkhead box D2) gene expression in Xenopus laevis embryo via in situ hybridization, NF stage 27, lateral view, anteriorright, dorsal up.
foxd2 (forkhead box D2) gene expression in Xenopus laevis embryo via in situ hybridization, NF stage 15, dorsal view, anteriorright.
foxd2 (forkhead box D2) gene expression in Xenopus laevis embryo via in situ hybridization, NF stage 21, dorsal view, anteriorright.
foxd1 () gene expression in Xenopus laevis embryo via in situ hybridization, NF stage 13 (top), anterodorsal view, dorsal up, and NF stage 20/21 (bottom) lateral view, anteriorleft, dorsal up.
