Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Mol Cell Biol
2006 Feb 01;263:1098-108. doi: 10.1128/MCB.26.3.1098-1108.2006.
Show Gene links
Show Anatomy links
Regulation of replication licensing by acetyltransferase Hbo1.
Iizuka M
,
Matsui T
,
Takisawa H
,
Smith MM
.
???displayArticle.abstract???
The initiation of DNA replication is tightly regulated in eukaryotic cells to ensure that the genome is precisely duplicated once and only once per cell cycle. This is accomplished by controlling the assembly of a prereplicative complex (pre-RC) which involves the sequential binding to replication origins of the origin recognition complex (ORC), Cdc6/Cdc18, Cdt1, and the minichromosome maintenance complex (Mcm2-Mcm7, or Mcm2-7). Several mechanisms of pre-RC regulation are known, including ATP utilization, cyclin-dependent kinase levels, protein turnover, and Cdt1 binding by geminin. Histone acetylation may also affect the initiation of DNA replication, but at present neither the enzymes nor the steps involved are known. Here, we show that Hbo1, a member of the MYST histone acetyltransferase family, is a previously unrecognized positive regulatory factor for pre-RC assembly. When Hbo1 expression was inhibited in human cells, Mcm2-7 failed to associate with chromatin even though ORC and Cdc6 loading was normal. When Xenopus egg extracts were immunodepleted of Xenopus Hbo1 (XHbo1), chromatin binding of Mcm2-7 was lost, and DNA replication was abolished. The binding of Mcm2-7 to chromatin in XHbo1-depleted extracts could be restored by the addition of recombinant Cdt1.
Aggarwal,
Chromatin regulates origin activity in Drosophila follicle cells.
2004,
Pubmed
Alexandrow,
Cdc6 chromatin affinity is unaffected by serine-54 phosphorylation, S-phase progression, and overexpression of cyclin A.
2004,
Pubmed
Alexandrow,
c-myc-enhanced S phase entry in keratinocytes is associated with positive and negative effects on cyclin-dependent kinases.
1998,
Pubmed
Aparicio,
The Rpd3-Sin3 histone deacetylase regulates replication timing and enables intra-S origin control in Saccharomyces cerevisiae.
2004,
Pubmed
Bell,
ATP-dependent recognition of eukaryotic origins of DNA replication by a multiprotein complex.
1992,
Pubmed
Bell,
DNA replication in eukaryotic cells.
2002,
Pubmed
Blow,
Preventing re-replication of chromosomal DNA.
2005,
Pubmed
Blow,
A role for the nuclear envelope in controlling DNA replication within the cell cycle.
1988,
Pubmed
,
Xenbase
Brown,
The chromatin structure of Saccharomyces cerevisiae autonomously replicating sequences changes during the cell division cycle.
1991,
Pubmed
Burke,
Replication factors MCM2 and ORC1 interact with the histone acetyltransferase HBO1.
2001,
Pubmed
Coleman,
The Xenopus Cdc6 protein is essential for the initiation of a single round of DNA replication in cell-free extracts.
1996,
Pubmed
,
Xenbase
Danis,
Specification of a DNA replication origin by a transcription complex.
2004,
Pubmed
,
Xenbase
Diffley,
Regulation of early events in chromosome replication.
2004,
Pubmed
,
Xenbase
Donovan,
Cdc6p-dependent loading of Mcm proteins onto pre-replicative chromatin in budding yeast.
1997,
Pubmed
,
Xenbase
Elbashir,
Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells.
2001,
Pubmed
Gavin,
Conserved initiator proteins in eukaryotes.
1995,
Pubmed
Gerhard,
The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).
2004,
Pubmed
,
Xenbase
Hardy,
Construction of adenovirus vectors through Cre-lox recombination.
1997,
Pubmed
Henneke,
Multiple roles for kinases in DNA replication.
2003,
Pubmed
Hodgson,
Geminin becomes activated as an inhibitor of Cdt1/RLF-B following nuclear import.
2002,
Pubmed
,
Xenbase
Iizuka,
Histone acetyltransferase HBO1 interacts with the ORC1 subunit of the human initiator protein.
1999,
Pubmed
Kelly,
Regulation of chromosome replication.
2000,
Pubmed
Kubota,
Determination of initiation of DNA replication before and after nuclear formation in Xenopus egg cell free extracts.
1993,
Pubmed
,
Xenbase
Li,
Cdt1 downregulation by proteolysis and geminin inhibition prevents DNA re-replication in Xenopus.
2005,
Pubmed
,
Xenbase
Li,
Non-proteolytic inactivation of geminin requires CDK-dependent ubiquitination.
2004,
Pubmed
,
Xenbase
Lipford,
Nucleosomes positioned by ORC facilitate the initiation of DNA replication.
2001,
Pubmed
Lu,
Generation and characterization of novel antibodies highly selective for phosphorylated linker histone H1 in Tetrahymena and HeLa cells.
1994,
Pubmed
Machida,
Right place, right time, and only once: replication initiation in metazoans.
2005,
Pubmed
Maiorano,
XCDT1 is required for the assembly of pre-replicative complexes in Xenopus laevis.
2000,
Pubmed
,
Xenbase
McGarry,
Geminin, an inhibitor of DNA replication, is degraded during mitosis.
1998,
Pubmed
,
Xenbase
Megee,
Genetic analysis of histone H4: essential role of lysines subject to reversible acetylation.
1990,
Pubmed
Melixetian,
Loss of Geminin induces rereplication in the presence of functional p53.
2004,
Pubmed
Méndez,
Chromatin association of human origin recognition complex, cdc6, and minichromosome maintenance proteins during the cell cycle: assembly of prereplication complexes in late mitosis.
2000,
Pubmed
,
Xenbase
Mihaylov,
Control of DNA replication and chromosome ploidy by geminin and cyclin A.
2002,
Pubmed
Nevins,
Functional analysis of E2F transcription factor.
1997,
Pubmed
Nguyen,
Cyclin-dependent kinases prevent DNA re-replication through multiple mechanisms.
2001,
Pubmed
Nishitani,
The Cdt1 protein is required to license DNA for replication in fission yeast.
2000,
Pubmed
Pagano,
Cyclin D1-mediated inhibition of repair and replicative DNA synthesis in human fibroblasts.
1994,
Pubmed
Pappas,
The NAD(+)-dependent Sir2p histone deacetylase is a negative regulator of chromosomal DNA replication.
2004,
Pubmed
Saxena,
A dimerized coiled-coil domain and an adjoining part of geminin interact with two sites on Cdt1 for replication inhibition.
2004,
Pubmed
,
Xenbase
Simpson,
Nucleosome positioning can affect the function of a cis-acting DNA element in vivo.
1990,
Pubmed
Stedman,
ORC, MCM, and histone hyperacetylation at the Kaposi's sarcoma-associated herpesvirus latent replication origin.
2004,
Pubmed
Suomalainen,
Microtubule-dependent plus- and minus end-directed motilities are competing processes for nuclear targeting of adenovirus.
1999,
Pubmed
Tada,
Repression of origin assembly in metaphase depends on inhibition of RLF-B/Cdt1 by geminin.
2001,
Pubmed
,
Xenbase
Utley,
The MYST family of histone acetyltransferases.
2003,
Pubmed
Vogelauer,
Histone acetylation regulates the time of replication origin firing.
2002,
Pubmed
Wohlschlegel,
Inhibition of eukaryotic DNA replication by geminin binding to Cdt1.
2000,
Pubmed
,
Xenbase
Yang,
The diverse superfamily of lysine acetyltransferases and their roles in leukemia and other diseases.
2004,
Pubmed
Yoshida,
Intrinsic nuclear import activity of geminin is essential to prevent re-initiation of DNA replication in Xenopus eggs.
2005,
Pubmed
,
Xenbase
Zhu,
Rereplication by depletion of geminin is seen regardless of p53 status and activates a G2/M checkpoint.
2004,
Pubmed
