Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
EMBO J
2001 Jun 01;2011:2802-11. doi: 10.1093/emboj/20.11.2802.
Show Gene links
Show Anatomy links
Hormone-induced nucleosome positioning in the MMTV promoter is reversible.
Belikov S
,
Gelius B
,
Wrange O
.
???displayArticle.abstract???
The mouse mammary tumor virus (MMTV) promoter is induced by glucocorticoid hormone via the glucocorticoid receptor (GR). The hormone-triggered effects on MMTV transcription and chromatin structure were studied in Xenopus oocytes. We previously showed that the nucleosomes organizing the MMTV promoter became translationally positioned upon hormone induction. A single GR-binding site was necessary and sufficient for the chromatin events to occur, while transcription and basal promoter elements were dispensable. Here we show that addition of the hormone antagonists RU486 or RU43044 to the previously hormone-induced MMTV promoter results in cessation of transcription and loss of chromatin remodeling and nucleosome positioning. In vivo footprinting demonstrated agonist- and RU486-induced GR binding to its DNA response element (GRE), while the other antagonist, RU43044, did not promote GR-GRE interaction. These results demonstrate that induction and maintenance of nucleosome positioning is an active process that requires constant 'pressure' of agonist-GR-recruited chromatin-modifying factor(s) rather than GR-DNA binding itself.
Agalioti,
Ordered recruitment of chromatin modifying and general transcription factors to the IFN-beta promoter.
2000, Pubmed
Agalioti,
Ordered recruitment of chromatin modifying and general transcription factors to the IFN-beta promoter.
2000,
Pubmed
Almouzni,
Replication-coupled chromatin assembly is required for the repression of basal transcription in vivo.
1993,
Pubmed
,
Xenbase
Archer,
Transcription factor access is mediated by accurately positioned nucleosomes on the mouse mammary tumor virus promoter.
1991,
Pubmed
Beato,
Transcription factor access to chromatin.
1997,
Pubmed
Becker,
In vivo protein-DNA interactions in a glucocorticoid response element require the presence of the hormone.
,
Pubmed
Belikov,
Hormone activation induces nucleosome positioning in vivo.
2000,
Pubmed
,
Xenbase
Bocquel,
Type II antagonists impair the DNA binding of steroid hormone receptors without affecting dimerization.
1993,
Pubmed
Cadepond,
RU486 (mifepristone): mechanisms of action and clinical uses.
1997,
Pubmed
Cirillo,
An early developmental transcription factor complex that is more stable on nucleosome core particles than on free DNA.
1999,
Pubmed
Clark,
Superhelical stress and nucleosome-mediated repression of 5S RNA gene transcription in vitro.
1991,
Pubmed
,
Xenbase
DiRenzo,
BRG-1 is recruited to estrogen-responsive promoters and cooperates with factors involved in histone acetylation.
2000,
Pubmed
El-Osta,
DNA methylation and histone deacetylation in the control of gene expression: basic biochemistry to human development and disease.
2000,
Pubmed
Eriksson,
Protein-protein contacts in the glucocorticoid receptor homodimer influence its DNA binding properties.
1990,
Pubmed
Flaus,
Positioning and stability of nucleosomes on MMTV 3'LTR sequences.
1998,
Pubmed
Fragoso,
Nucleosome positioning on the MMTV LTR results from the frequency-biased occupancy of multiple frames.
1995,
Pubmed
Fragoso,
The position and length of the steroid-dependent hypersensitive region in the mouse mammary tumor virus long terminal repeat are invariant despite multiple nucleosome B frames.
1998,
Pubmed
Fryer,
Chromatin remodelling by the glucocorticoid receptor requires the BRG1 complex.
1998,
Pubmed
Gelius,
Characterization of a chromatin remodelling activity in Xenopus oocytes.
1999,
Pubmed
,
Xenbase
Germond,
Folding of the DNA double helix in chromatin-like structures from simian virus 40.
1975,
Pubmed
Guiochon-Mantel,
Receptors bound to antiprogestin from abortive complexes with hormone responsive elements.
1988,
Pubmed
Haché,
Nucleocytoplasmic trafficking of steroid-free glucocorticoid receptor.
1999,
Pubmed
Hager,
Understanding nuclear receptor function: from DNA to chromatin to the interphase nucleus.
2001,
Pubmed
Hong,
GRIP1, a novel mouse protein that serves as a transcriptional coactivator in yeast for the hormone binding domains of steroid receptors.
1996,
Pubmed
Huber,
Genomic position effects lead to an inefficient reorganization of nucleosomes in the 5'-regulatory region of the chicken lysozyme locus in transgenic mice.
1996,
Pubmed
Kingston,
ATP-dependent remodeling and acetylation as regulators of chromatin fluidity.
1999,
Pubmed
Klein-Hitpass,
Two types of antiprogestins identified by their differential action in transcriptionally active extracts from T47D cells.
1991,
Pubmed
Lemon,
Orchestrated response: a symphony of transcription factors for gene control.
2000,
Pubmed
Lu,
Nucleosome positioning and gene regulation.
1994,
Pubmed
Luger,
Crystal structure of the nucleosome core particle at 2.8 A resolution.
1997,
Pubmed
Maxam,
A new method for sequencing DNA.
1977,
Pubmed
McNally,
The glucocorticoid receptor: rapid exchange with regulatory sites in living cells.
2000,
Pubmed
McPherson,
An active tissue-specific enhancer and bound transcription factors existing in a precisely positioned nucleosomal array.
1993,
Pubmed
Mymryk,
Dissection of progesterone receptor-mediated chromatin remodeling and transcriptional activation in vivo.
1995,
Pubmed
Norton,
Histone acetylation reduces nucleosome core particle linking number change.
1989,
Pubmed
Ohlsson,
Sequence-specific interactions of nuclear factors with the insulin gene enhancer.
1986,
Pubmed
Perlmann,
Specific glucocorticoid receptor binding to DNA reconstituted in a nucleosome.
1988,
Pubmed
Peterson,
Promoter targeting and chromatin remodeling by the SWI/SNF complex.
2000,
Pubmed
Picard,
Two signals mediate hormone-dependent nuclear localization of the glucocorticoid receptor.
1987,
Pubmed
Piña,
Nucleosome positioning modulates accessibility of regulatory proteins to the mouse mammary tumor virus promoter.
1990,
Pubmed
Richard-Foy,
Sequence-specific positioning of nucleosomes over the steroid-inducible MMTV promoter.
1987,
Pubmed
Roberts,
Nucleosomes reconstituted in vitro on mouse mammary tumor virus B region DNA occupy multiple translational and rotational frames.
1995,
Pubmed
Robinett,
Modeling transcriptional regulation using microinjection into Xenopus oocytes.
1999,
Pubmed
,
Xenbase
Rousseau,
Structure-activity relationships for glucocorticoids-I. Determination of receptor binding and biological activity.
1977,
Pubmed
Shang,
Cofactor dynamics and sufficiency in estrogen receptor-regulated transcription.
2000,
Pubmed
Shim,
Nucleosome positioning by the winged helix transcription factor HNF3.
1998,
Pubmed
Simpson,
Nucleosome positioning: occurrence, mechanisms, and functional consequences.
1991,
Pubmed
Strahl,
The language of covalent histone modifications.
2000,
Pubmed
Truss,
Antiprogestins prevent progesterone receptor binding to hormone responsive elements in vivo.
1994,
Pubmed
Truss,
Hormone induces binding of receptors and transcription factors to a rearranged nucleosome on the MMTV promoter in vivo.
1995,
Pubmed
Urnov,
Targeting of N-CoR and histone deacetylase 3 by the oncoprotein v-erbA yields a chromatin infrastructure-dependent transcriptional repression pathway.
2000,
Pubmed
,
Xenbase
Venditti,
Assembly of MMTV promoter minichromosomes with positioned nucleosomes precludes NF1 access but not restriction enzyme cleavage.
1998,
Pubmed
Webster,
The hormone-binding domains of the estrogen and glucocorticoid receptors contain an inducible transcription activation function.
1988,
Pubmed
Wolffe,
Review: chromatin structural features and targets that regulate transcription.
2000,
Pubmed
Zaret,
Reversible and persistent changes in chromatin structure accompany activation of a glucocorticoid-dependent enhancer element.
1984,
Pubmed
