Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
LAP2 binds to BAF.DNA complexes: requirement for the LEM domain and modulation by variable regions.
Shumaker DK
,
Lee KK
,
Tanhehco YC
,
Craigie R
,
Wilson KL
.
???displayArticle.abstract???
LAP2 belongs to a family of nuclear membrane proteins sharing a 43 residue LEM domain. All LAP2 isoforms have the same N-terminal 'constant' region (LAP2-c), which includes the LEM domain, plus a C-terminal 'variable' region. LAP2-c polypeptide inhibits nuclear assembly in Xenopus extracts, and binds in vitro to barrier-to-autointegration factor (BAF), a DNA-bridging protein. We tested 17 Xenopus LAP2-c mutants for nuclear assembly inhibition, and binding to BAF and BAF small middle dotDNA complexes. LEM domain mutations disrupted all activities tested. Some mutations outside the LEM domain had no effect on binding to BAF, but disrupted activity in Xenopus extracts, suggesting that LAP2-c has an additional unknown function required to inhibit nuclear assembly. Mutagenesis results suggest that BAF changes conformation when complexed with DNA. The binding affinity of LAP2 was higher for BAF small middle dotDNA complexes than for BAF, suggesting that these interactions are physiologically relevant. Nucleoplasmic domains of Xenopus LAP2 isoforms varied 9-fold in their affinities for BAF, but all isoforms supershifted BAF small middle dotDNA complexes. We propose that the LEM domain is a core BAF-binding domain that can be modulated by the variable regions of LAP2 isoforms.
Berger,
The characterization and localization of the mouse thymopoietin/lamina-associated polypeptide 2 gene and its alternatively spliced products.
1996, Pubmed
Berger,
The characterization and localization of the mouse thymopoietin/lamina-associated polypeptide 2 gene and its alternatively spliced products.
1996,
Pubmed
Bione,
Identification of a novel X-linked gene responsible for Emery-Dreifuss muscular dystrophy.
1994,
Pubmed
Bonne,
Mutations in the gene encoding lamin A/C cause autosomal dominant Emery-Dreifuss muscular dystrophy.
1999,
Pubmed
Bonne,
Clinical and molecular genetic spectrum of autosomal dominant Emery-Dreifuss muscular dystrophy due to mutations of the lamin A/C gene.
2000,
Pubmed
Cai,
Solution structure of the cellular factor BAF responsible for protecting retroviral DNA from autointegration.
1998,
Pubmed
Chen,
The barrier-to-autointegration protein is a host factor for HIV type 1 integration.
1998,
Pubmed
Clements,
Direct interaction between emerin and lamin A.
2000,
Pubmed
Cohen,
Transcriptional repression, apoptosis, human disease and the functional evolution of the nuclear lamina.
2001,
Pubmed
Dechat,
Lamina-associated polypeptide 2alpha binds intranuclear A-type lamins.
2000,
Pubmed
Dechat,
Detergent-salt resistance of LAP2alpha in interphase nuclei and phosphorylation-dependent association with chromosomes early in nuclear assembly implies functions in nuclear structure dynamics.
1998,
Pubmed
Dechat,
Review: lamina-associated polypeptide 2 isoforms and related proteins in cell cycle-dependent nuclear structure dynamics.
2000,
Pubmed
,
Xenbase
Dreger,
Identification of phosphorylation sites in native lamina-associated polypeptide 2 beta.
1999,
Pubmed
Duband-Goulet,
Inner nuclear membrane protein LBR preferentially interacts with DNA secondary structures and nucleosomal linker.
2000,
Pubmed
Foisner,
Integral membrane proteins of the nuclear envelope interact with lamins and chromosomes, and binding is modulated by mitotic phosphorylation.
1993,
Pubmed
Furukawa,
Cloning of a cDNA for lamina-associated polypeptide 2 (LAP2) and identification of regions that specify targeting to the nuclear envelope.
1995,
Pubmed
Furukawa,
The major nuclear envelope targeting domain of LAP2 coincides with its lamin binding region but is distinct from its chromatin interaction domain.
1998,
Pubmed
Furukawa,
Characterization of the chromatin binding activity of lamina-associated polypeptide (LAP) 2.
1997,
Pubmed
Furukawa,
LAP2 binding protein 1 (L2BP1/BAF) is a candidate mediator of LAP2-chromatin interaction.
1999,
Pubmed
Gant,
Roles of LAP2 proteins in nuclear assembly and DNA replication: truncated LAP2beta proteins alter lamina assembly, envelope formation, nuclear size, and DNA replication efficiency in Xenopus laevis extracts.
1999,
Pubmed
,
Xenbase
Gant,
Nuclear assembly.
1997,
Pubmed
Goldberg,
Interactions among Drosophila nuclear envelope proteins lamin, otefin, and YA.
1998,
Pubmed
Gruenbaum,
Review: nuclear lamins--structural proteins with fundamental functions.
2000,
Pubmed
Harris,
Three distinct human thymopoietins are derived from alternatively spliced mRNAs.
1994,
Pubmed
Lang,
Molecular characterization and developmentally regulated expression of Xenopus lamina-associated polypeptide 2 (XLAP2).
1999,
Pubmed
,
Xenbase
Lee,
A previously unidentified host protein protects retroviral DNA from autointegration.
1998,
Pubmed
Lee,
C. elegans nuclear envelope proteins emerin, MAN1, lamin, and nucleoporins reveal unique timing of nuclear envelope breakdown during mitosis.
2000,
Pubmed
Lenz-Böhme,
Insertional mutation of the Drosophila nuclear lamin Dm0 gene results in defective nuclear envelopes, clustering of nuclear pore complexes, and accumulation of annulate lamellae.
1997,
Pubmed
Lin,
MAN1, an inner nuclear membrane protein that shares the LEM domain with lamina-associated polypeptide 2 and emerin.
2000,
Pubmed
Liu,
Essential roles for Caenorhabditis elegans lamin gene in nuclear organization, cell cycle progression, and spatial organization of nuclear pore complexes.
2000,
Pubmed
Manilal,
The Emery-Dreifuss muscular dystrophy protein, emerin, is a nuclear membrane protein.
1996,
Pubmed
Moir,
Review: the dynamics of the nuclear lamins during the cell cycle-- relationship between structure and function.
2000,
Pubmed
Morris,
Heart to heart: from nuclear proteins to Emery-Dreifuss muscular dystrophy.
1999,
Pubmed
Newmeyer,
Egg extracts for nuclear import and nuclear assembly reactions.
1991,
Pubmed
,
Xenbase
Padan,
Isolation and characterization of the Drosophila nuclear envelope otefin cDNA.
1990,
Pubmed
Sullivan,
Loss of A-type lamin expression compromises nuclear envelope integrity leading to muscular dystrophy.
1999,
Pubmed
Umland,
Structural basis of DNA bridging by barrier-to-autointegration factor.
2000,
Pubmed
Vlcek,
Functional diversity of LAP2alpha and LAP2beta in postmitotic chromosome association is caused by an alpha-specific nuclear targeting domain.
1999,
Pubmed
Wang,
Purification and biochemical heterogeneity of the mammalian SWI-SNF complex.
1996,
Pubmed
Wilson,
The nuclear envelope, muscular dystrophy and gene expression.
2000,
Pubmed
Yang,
Lamin-binding fragment of LAP2 inhibits increase in nuclear volume during the cell cycle and progression into S phase.
1997,
Pubmed
Ye,
Primary structure analysis and lamin B and DNA binding of human LBR, an integral protein of the nuclear envelope inner membrane.
1994,
Pubmed
Ye,
Interaction between an integral protein of the nuclear envelope inner membrane and human chromodomain proteins homologous to Drosophila HP1.
1996,
Pubmed
Zheng,
Barrier-to-autointegration factor (BAF) bridges DNA in a discrete, higher-order nucleoprotein complex.
2000,
Pubmed
