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The ATPase ISWI can be considered the catalytic core of several multiprotein nucleosome remodeling machines. Alone or in the context of nucleosome remodeling factor, the chromatin accessibility complex (CHRAC), or ACF, ISWI catalyzes a number of ATP-dependent transitions of chromatin structure that are currently best explained by its ability to induce nucleosome sliding. In addition, ISWI can function as a nucleosome spacing factor during chromatin assembly, where it will trigger the ordering of newly assembled nucleosomes into regular arrays. Both nucleosome remodeling and nucleosome spacing reactions are mechanistically unexplained. As a step toward defining the interaction of ISWI with its substrate during nucleosome remodeling and chromatin assembly we generated a set of nucleosomes lacking individual histone N termini from recombinant histones. We found the conserved N termini (the N-terminal tails) of histone H4 essential to stimulate ISWI ATPase activity, in contrast to other histone tails. Remarkably, the H4 N terminus, but none of the other tails, was critical for CHRAC-induced nucleosome sliding and for the generation of regularity in nucleosomal arrays by ISWI. Direct nucleosome binding studies did not reflect a dependence on the H4 tail for ISWI-nucleosome interactions. We conclude that the H4 tail is critically required for nucleosome remodeling and spacing at a step subsequent to interaction with the substrate.
Akhtar,
Activation of transcription through histone H4 acetylation by MOF, an acetyltransferase essential for dosage compensation in Drosophila.
2000, Pubmed
Akhtar,
Activation of transcription through histone H4 acetylation by MOF, an acetyltransferase essential for dosage compensation in Drosophila.
2000,
Pubmed
Bone,
Acetylated histone H4 on the male X chromosome is associated with dosage compensation in Drosophila.
1994,
Pubmed
Boyer,
Functional delineation of three groups of the ATP-dependent family of chromatin remodeling enzymes.
2000,
Pubmed
Brehm,
dMi-2 and ISWI chromatin remodelling factors have distinct nucleosome binding and mobilization properties.
2000,
Pubmed
Breiling,
The Drosophila polycomb protein interacts with nucleosomal core particles In vitro via its repression domain.
1999,
Pubmed
Corona,
ISWI is an ATP-dependent nucleosome remodeling factor.
1999,
Pubmed
Dhalluin,
Structure and ligand of a histone acetyltransferase bromodomain.
1999,
Pubmed
Durrin,
Yeast histone H4 N-terminal sequence is required for promoter activation in vivo.
1991,
Pubmed
Edmondson,
Repression domain of the yeast global repressor Tup1 interacts directly with histones H3 and H4.
1996,
Pubmed
Eisen,
Evolution of the SNF2 family of proteins: subfamilies with distinct sequences and functions.
1995,
Pubmed
Elfring,
Identification and characterization of Drosophila relatives of the yeast transcriptional activator SNF2/SWI2.
1994,
Pubmed
Fletcher,
Core histone tail domains mediate oligonucleosome folding and nucleosomal DNA organization through distinct molecular mechanisms.
1995,
Pubmed
Garcia-Ramirez,
Role of the histone "tails" in the folding of oligonucleosomes depleted of histone H1.
1992,
Pubmed
Georgel,
Role of histone tails in nucleosome remodeling by Drosophila NURF.
1997,
Pubmed
Grunstein,
Histone acetylation in chromatin structure and transcription.
1997,
Pubmed
Guschin,
ATP-Dependent histone octamer mobilization and histone deacetylation mediated by the Mi-2 chromatin remodeling complex.
2000,
Pubmed
,
Xenbase
Guyon,
Stable remodeling of tailless nucleosomes by the human SWI-SNF complex.
1999,
Pubmed
Hamiche,
ATP-dependent histone octamer sliding mediated by the chromatin remodeling complex NURF.
1999,
Pubmed
Hansen,
Structure and function of the core histone N-termini: more than meets the eye.
1998,
Pubmed
Hansen,
Influence of chromatin folding on transcription initiation and elongation by RNA polymerase III.
1992,
Pubmed
,
Xenbase
Hecht,
Histone H3 and H4 N-termini interact with SIR3 and SIR4 proteins: a molecular model for the formation of heterochromatin in yeast.
1995,
Pubmed
Huang,
Amino termini of histones H3 and H4 are required for a1-alpha2 repression in yeast.
1997,
Pubmed
Ito,
ACF consists of two subunits, Acf1 and ISWI, that function cooperatively in the ATP-dependent catalysis of chromatin assembly.
1999,
Pubmed
Ito,
ACF, an ISWI-containing and ATP-utilizing chromatin assembly and remodeling factor.
1997,
Pubmed
Kayne,
Extremely conserved histone H4 N terminus is dispensable for growth but essential for repressing the silent mating loci in yeast.
1988,
Pubmed
Kingston,
ATP-dependent remodeling and acetylation as regulators of chromatin fluidity.
1999,
Pubmed
Längst,
Nucleosome movement by CHRAC and ISWI without disruption or trans-displacement of the histone octamer.
1999,
Pubmed
LeRoy,
Requirement of RSF and FACT for transcription of chromatin templates in vitro.
1998,
Pubmed
Logie,
The core histone N-terminal domains are required for multiple rounds of catalytic chromatin remodeling by the SWI/SNF and RSC complexes.
1999,
Pubmed
Lorch,
Histone octamer transfer by a chromatin-remodeling complex.
1999,
Pubmed
,
Xenbase
Lorch,
Activated RSC-nucleosome complex and persistently altered form of the nucleosome.
1998,
Pubmed
Luger,
Characterization of nucleosome core particles containing histone proteins made in bacteria.
1997,
Pubmed
,
Xenbase
Luger,
Preparation of nucleosome core particle from recombinant histones.
1999,
Pubmed
,
Xenbase
Luger,
Crystal structure of the nucleosome core particle at 2.8 A resolution.
1997,
Pubmed
Ornaghi,
The bromodomain of Gcn5p interacts in vitro with specific residues in the N terminus of histone H4.
1999,
Pubmed
Peterson,
Multiple SWItches to turn on chromatin?
1996,
Pubmed
Phelan,
Reconstitution of a core chromatin remodeling complex from SWI/SNF subunits.
1999,
Pubmed
Sandaltzopoulos,
A solid-phase approach for the analysis of reconstituted chromatin.
1999,
Pubmed
,
Xenbase
Schnitzler,
Human SWI/SNF interconverts a nucleosome between its base state and a stable remodeled state.
1998,
Pubmed
Strahl,
The language of covalent histone modifications.
2000,
Pubmed
Tong,
Chromatin deacetylation by an ATP-dependent nucleosome remodelling complex.
1998,
Pubmed
Tse,
Disruption of higher-order folding by core histone acetylation dramatically enhances transcription of nucleosomal arrays by RNA polymerase III.
1998,
Pubmed
,
Xenbase
Tsukiyama,
Characterization of the imitation switch subfamily of ATP-dependent chromatin-remodeling factors in Saccharomyces cerevisiae.
1999,
Pubmed
Tsukiyama,
ISWI, a member of the SWI2/SNF2 ATPase family, encodes the 140 kDa subunit of the nucleosome remodeling factor.
1995,
Pubmed
Turner,
Histone H4 isoforms acetylated at specific lysine residues define individual chromosomes and chromatin domains in Drosophila polytene nuclei.
1992,
Pubmed
Turner,
Histone acetylation as an epigenetic determinant of long-term transcriptional competence.
1998,
Pubmed
Varga-Weisz,
Chromatin-remodelling factor CHRAC contains the ATPases ISWI and topoisomerase II.
1997,
Pubmed
Vignali,
ATP-dependent chromatin-remodeling complexes.
2000,
Pubmed
Whitehouse,
Nucleosome mobilization catalysed by the yeast SWI/SNF complex.
1999,
Pubmed
Winston,
The bromodomain: a chromatin-targeting module?
1999,
Pubmed
Wolffe,
Epigenetics: regulation through repression.
1999,
Pubmed
Woodage,
Characterization of the CHD family of proteins.
1997,
Pubmed
Workman,
Alteration of nucleosome structure as a mechanism of transcriptional regulation.
1998,
Pubmed
Xue,
NURD, a novel complex with both ATP-dependent chromatin-remodeling and histone deacetylase activities.
1998,
Pubmed
Zhang,
The dermatomyositis-specific autoantigen Mi2 is a component of a complex containing histone deacetylase and nucleosome remodeling activities.
1998,
Pubmed
