Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Mol Biol Cell
2000 Dec 01;1112:4143-58. doi: 10.1091/mbc.11.12.4143.
Show Gene links
Show Anatomy links
Expression and partial characterization of kinesin-related proteins in differentiating and adult skeletal muscle.
Ginkel LM
,
Wordeman L
.
???displayArticle.abstract???
Using pan-kinesin antibodies to screen a differentiating C2C12 cell library, we identified the kinesin proteins KIF3A, KIF3B, and conventional kinesin heavy chain to be present in differentiating skeletal muscle. We compared the expression and subcellular localization characteristics of these kinesins in myogenic cells to others previously identified in muscle, neuronal, and mitotic systems (KIF1C, KIF3C, and mitotic-centromere-associated kinesin). Because members of the KIF3 subfamily of kinesin-related proteins showed altered subcellular fractionation characteristics in differentiating cells, we focused our study of kinesins in muscle on the function of kinesin-II. Kinesin-II is a motor complex comprised of dimerized KIF3A and KIF3B proteins and a tail-associated protein, KAP. The Xenopus homologue of KIF3B, Xklp3, is predominantly localized to the region of the Golgi apparatus, and overexpression of motorless-Xklp3 in Xenopus A6 cells causes mislocalization of Golgi components (). In C2C12 myoblasts and myotubes, KIF3B is diffuse and punctate, and not primarily associated with the Golgi. Overexpression of motorless-KIF3B does not perturb localization of Golgi components in myogenic cells, and myofibrillogenesis is normal. In adult skeletal muscle, KIF3B colocalizes with the excitation-contraction-coupling membranes. We propose that these membranes, consisting of the transverse-tubules and sarcoplasmic reticulum, are dynamic structures in which kinesin-II may function to actively assemble and maintain in myogenic cells.
Aizawa,
Kinesin family in murine central nervous system.
1992, Pubmed
Aizawa,
Kinesin family in murine central nervous system.
1992,
Pubmed
Antin,
Taxol induces postmitotic myoblasts to assemble interdigitating microtubule-myosin arrays that exclude actin filaments.
1981,
Pubmed
Ben-Ze'ev,
Mechanisms of regulating tubulin synthesis in cultured mammalian cells.
1979,
Pubmed
Bischoff,
The effect of mitotic inhibitors on myogenesis in vitro.
1968,
Pubmed
Bischoff,
The effect of mitotic inhibitors in myogenesis in vitro.
2009,
Pubmed
Brady,
A novel brain ATPase with properties expected for the fast axonal transport motor.
,
Pubmed
Chomczynski,
Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction.
1987,
Pubmed
Cleveland,
Unpolymerized tubulin modulates the level of tubulin mRNAs.
1981,
Pubmed
Cole,
Isolation of a sea urchin egg kinesin-related protein using peptide antibodies.
1992,
Pubmed
,
Xenbase
Cole,
Novel heterotrimeric kinesin-related protein purified from sea urchin eggs.
1993,
Pubmed
,
Xenbase
Cole,
Kinesin-II, coming and going.
1999,
Pubmed
Cole,
Chlamydomonas kinesin-II-dependent intraflagellar transport (IFT): IFT particles contain proteins required for ciliary assembly in Caenorhabditis elegans sensory neurons.
1998,
Pubmed
Dorner,
Characterization of KIF1C, a new kinesin-like protein involved in vesicle transport from the Golgi apparatus to the endoplasmic reticulum.
1998,
Pubmed
Faire,
Identification of kinesin-like molecules in myogenic cells.
1998,
Pubmed
Flucher,
Coordinated development of myofibrils, sarcoplasmic reticulum and transverse tubules in normal and dysgenic mouse skeletal muscle, in vivo and in vitro.
1992,
Pubmed
Flucher,
Formation of junctions involved in excitation-contraction coupling in skeletal and cardiac muscle.
1996,
Pubmed
Flucher,
Development of the excitation-contraction coupling apparatus in skeletal muscle: association of sarcoplasmic reticulum and transverse tubules with myofibrils.
1993,
Pubmed
Ginkel,
Expression cloning with pan kinesin antibodies.
2001,
Pubmed
Goldstein,
The road less traveled: emerging principles of kinesin motor utilization.
1999,
Pubmed
Gundersen,
Generation of a stable, posttranslationally modified microtubule array is an early event in myogenic differentiation.
1989,
Pubmed
Hancock,
Processivity of the motor protein kinesin requires two heads.
1998,
Pubmed
Hirokawa,
Kinesin and dynein superfamily proteins and the mechanism of organelle transport.
1998,
Pubmed
Hollenbeck,
The distribution, abundance and subcellular localization of kinesin.
1989,
Pubmed
Holtzer,
Effects of cytochaslasin B and colcemide on myogenic cultures.
1975,
Pubmed
Holtzer,
Myogenic lineages and myofibrillogenesis.
1986,
Pubmed
Kondo,
KIF3A is a new microtubule-based anterograde motor in the nerve axon.
1994,
Pubmed
Le Bot,
Role of xklp3, a subunit of the Xenopus kinesin II heterotrimeric complex, in membrane transport between the endoplasmic reticulum and the Golgi apparatus.
1998,
Pubmed
,
Xenbase
Lippincott-Schwartz,
Kinesin is the motor for microtubule-mediated Golgi-to-ER membrane traffic.
1995,
Pubmed
Maney,
Mitotic centromere-associated kinesin is important for anaphase chromosome segregation.
1998,
Pubmed
Mangan,
A muscle-specific variant of microtubule-associated protein 4 (MAP4) is required in myogenesis.
1996,
Pubmed
Marszalek,
Novel dendritic kinesin sorting identified by different process targeting of two related kinesins: KIF21A and KIF21B.
1999,
Pubmed
Moore,
Kinesin proteins: a phylum of motors for microtubule-based motility.
1996,
Pubmed
Muresan,
KIF3C and KIF3A form a novel neuronal heteromeric kinesin that associates with membrane vesicles.
1998,
Pubmed
Okada,
The neuron-specific kinesin superfamily protein KIF1A is a unique monomeric motor for anterograde axonal transport of synaptic vesicle precursors.
1995,
Pubmed
Parton,
Caveolin-3 associates with developing T-tubules during muscle differentiation.
1997,
Pubmed
Pavelka,
Localization of binding sites for concanavalin A, Ricinus communis I and Helix pomatia lectin in the Golgi apparatus of rat small intestinal absorptive cells.
1985,
Pubmed
Pfister,
Monoclonal antibodies to kinesin heavy and light chains stain vesicle-like structures, but not microtubules, in cultured cells.
1989,
Pubmed
Rafferty,
The length of secondary chromosomal constrictions in normal individuals and in a nucleolar mutant of Xenopus laevis.
1969,
Pubmed
,
Xenbase
Rahkila,
Endoplasmic reticulum to Golgi trafficking in multinucleated skeletal muscle fibers.
1997,
Pubmed
Rahkila,
Transport pathway, maturation, and targetting of the vesicular stomatitis virus glycoprotein in skeletal muscle fibers.
1996,
Pubmed
Ralston,
Changes in architecture of the Golgi complex and other subcellular organelles during myogenesis.
1993,
Pubmed
Ralston,
The organization of the Golgi complex and microtubules in skeletal muscle is fiber type-dependent.
1999,
Pubmed
Rappaport,
Microtubules in cardiac myocytes.
1988,
Pubmed
Rashid,
Heterodimerization of the two motor subunits of the heterotrimeric kinesin, KRP85/95.
1995,
Pubmed
Roth,
Cytochemical localization of terminal N-acetyl-D-galactosamine residues in cellular compartments of intestinal goblet cells: implications for the topology of O-glycosylation.
1984,
Pubmed
Roy,
Exercise induces the translocation of GLUT4 to transverse tubules from an intracellular pool in rat skeletal muscle.
1996,
Pubmed
Sawin,
Evidence for kinesin-related proteins in the mitotic apparatus using peptide antibodies.
1992,
Pubmed
,
Xenbase
Schaar,
CENP-E function at kinetochores is essential for chromosome alignment.
1997,
Pubmed
Thomas,
Newly synthesized calsequestrin, destined for the sarcoplasmic reticulum, is contained in early/intermediate Golgi-derived clathrin-coated vesicles.
1989,
Pubmed
Toyama,
Effects of taxol and Colcemid on myofibrillogenesis.
1982,
Pubmed
Tuma,
Heterotrimeric kinesin II is the microtubule motor protein responsible for pigment dispersion in Xenopus melanophores.
1998,
Pubmed
,
Xenbase
Vale,
The design plan of kinesin motors.
1997,
Pubmed
Vale,
Movement of organelles along filaments dissociated from the axoplasm of the squid giant axon.
1985,
Pubmed
Walker,
Evidence for connections of the sarcoplasmic reticulum with the sarcolemma and with the Z line in skeletal muscle fibers of fetal and newborn rats.
1969,
Pubmed
Wedaman,
Sequence and submolecular localization of the 115-kD accessory subunit of the heterotrimeric kinesin-II (KRP85/95) complex.
1996,
Pubmed
Weintraub,
The MyoD family and myogenesis: redundancy, networks, and thresholds.
1993,
Pubmed
Wood,
CENP-E is a plus end-directed kinetochore motor required for metaphase chromosome alignment.
1997,
Pubmed
,
Xenbase
Wordeman,
Identification and partial characterization of mitotic centromere-associated kinesin, a kinesin-related protein that associates with centromeres during mitosis.
1995,
Pubmed
Yaffe,
Serial passaging and differentiation of myogenic cells isolated from dystrophic mouse muscle.
,
Pubmed
Yamazaki,
KIF3A/B: a heterodimeric kinesin superfamily protein that works as a microtubule plus end-directed motor for membrane organelle transport.
1995,
Pubmed
Yang,
Characterization of the KIF3C neural kinesin-like motor from mouse.
1998,
Pubmed
