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XB-ANTIBODY-14580344
Antibody Name:
Trim36 Ab1
Common Name: RRID: Synonyms:
Source: |
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Antibody | |
Clone Number | |
Purification | |
Isotype | isotype unknown |
Host Organism | guinea pig |
Xenopus Reactivity | X. tropicalis, X. laevis |
Non-Xenopus Reactivity | |
Description: | Created by Cuykendall et al. The trim36 CDS was cloned into the pDEST17 vector (invitrogen) by Gateway recomb to generate a fusion protein N-terminally tagged with 6-His. Recombinant protein was produced in bacteria, and refolded from inclusion bodies as described (Houston and King, 2000a). The 6-His-tagged Trim36 protein was then purified over a nickel column (His GraviTrap, GE Healthcare), eluted with 300mM imidazole and dialyzed against PBS. |
Immunogen | |
Name | Trim36 |
Type | polypeptide |
Post Translational Modifications | None |
Source Organism | frog |
Description | |
Sequence | |
Reported Usage | |
Publications | |
???displayAntibody.papersFirst??? |
Vegetally localized Xenopus trim36 regulates cortical rotation and dorsal axis formation., Development 2009 |
???displayAntibody.papersRecent??? |
Maternal Dead-End1 is required for vegetal cortical microtubule assembly during Xenopus axis specification., Development 2013 |
???displayAntibody.papersAll??? |