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Antibody Name: Cdc45 Ab2 Common Name: RRID: Synonyms: Antigens Clone Type: unknown Source:

Antibody
Clone Number
Purification	Affinity Purification
Isotype	isotype unknown
Host Organism	sheep
Xenopus Reactivity	X. tropicalis, X. laevis
Non-Xenopus Reactivity
Description:	Generated in Blow's lab (2011). Cdc45 antibodies were raised in sheep against full-length His6-tagged X. laevis recombinant proteins expressed and purified from Escherichia coli (RosettaTM(DE3)pLysS, Novagen) using Ni2+-NTA affinity chromatography. Antibodies were used for immunoblotting at 1:1000 dilutions.
Immunogen
Name	cdc45
Type	polypeptide
Post Translational Modifications	None
Source Organism	frog
Description	Cdc45 antibodies were raised in sheep against full-length His6-tagged X. laevis recombinant proteins
Sequence
Reported Usage
western blot	1:1000
Publications
???displayAntibody.papersFirst???	MCM2-7 form double hexamers at licensed origins in Xenopus egg extract., J Biol Chem 2011
???displayAntibody.papersRecent???	The structural mechanism of dimeric DONSON in replicative helicase activation., Mol Cell 2023
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