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XB-ANTIBODY-19756688
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Antibody Name:
H3K27me3 Ab11
Common Name: Histone H3 (tri methyl K27) RRID: Synonyms:
Source: Abcam |
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| Antibody | |
| Clone Number | |
| Purification | Affinity Purification |
| Isotype | isotype unknown |
| Host Organism | mouse |
| Xenopus Reactivity | X. tropicalis, X. laevis |
| Non-Xenopus Reactivity | |
| Description: | Mouse monoclonal [mAbcam 6002] to Histone H3 (tri methyl K27) - ChIP Grade, aka Anti-Histone H3 (tri methyl K27) This antibody is specific for histone H3 tri-methylated at K27. The antibody is blocked in Western blot by tri methyl K27 peptide and slightly by di methyl K27 peptide (there is <12% cross-reactivity with di methyl K27 as determined by ELISA). |
| Immunogen | |
| Name | |
| Type | polypeptide |
| Post Translational Modifications | None |
| Source Organism | mouse |
| Description | Synthetic peptide within Human Histone H3 aa 1-100 (tri methyl K27) conjugated to keyhole limpet haemocyanin (Sulfosuccinimidyl 4-N-maleimidomethyl-cyclohexane-1-carboxylate (Sulfo-SMCC)). The exact sequence is proprietary. Clones were positively screened by ELISA against the immunizing peptide. Clones were negatively screened against both the unmodified peptide and also against tri methyl K9 Histone H3 peptide. (Peptide available as ab1782) |
| Sequence | |
| Reported Usage | |
| Publications | |
| ???displayAntibody.papersFirst??? |
The target of the NSD family of histone lysine methyltransferases depends on the nature of the substrate., J Biol Chem 2009  |
| ???displayAntibody.papersRecent??? | |
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