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Summary Attributions Wiki
XB-GRNA-23659090
gRNA Name: adprhl1 gRNA4
Synonyms: -e6-1
Genomic Target: adprhl1

PAM Sequence: AGG
Sequence: 5' GAGGGAAGAGGGGGAAGAAG 3'



Genomic Target tropicalis laevis.L laevis.S
Identities 20/20 20/20 20/20
genome
gRNA
 
5' CTTCTTCCCCCTCTTCCCTC 3'
   ||||||||||||||||||||
3' GAAGAAGGGGGAGAAGGGAG 5'
 
5' CTTCTTCCCCCTCTTCCCTC 3'
   ||||||||||||||||||||
3' GAAGAAGGGGGAGAAGGGAG 5'
 
5' CTTCTTCCCCCTCTTCCCTC 3'
   ||||||||||||||||||||
3' GAAGAAGGGGGAGAAGGGAG 5'
    
Genomic Alignments Position Identity Strand Target Genomic Target
laevis 10.1 Chr2L:120476164..120476183 20/20 Sense target adprhl1.L
Chr2S:106632018..106632037 20/20 Sense target adprhl1.S
Chr2S:75237052..75237071 19/20 Antisense off-target sf3a3.S
Chr9_10S:52139840..52139859 18/20 Sense off-target tns1.S
Chr3L:135736763..135736782 18/20 Sense off-target nefm.L
Chr1S:180169333..180169352 18/20 Antisense off-target MGC80748
Chr6L:5604221..5604240 18/20 Sense off-target LOC108718675
Chr9_10L:65367217..65367236 18/20 Antisense off-target LOC108701836
Chr3L:135763398..135763417 17/20 Antisense off-target nefl.L
Chr8L:102139094..102139113 17/20 Antisense off-target susd6.L
Chr4S:125487336..125487355 17/20 Antisense off-target ptprg.S
Chr4S:100740679..100740698 17/20 Sense off-target cyp2d6.S
Chr7L:67332344..67332363 17/20 Antisense off-target exosc10.L
Chr6L:146350462..146350481 17/20 Antisense off-target LOC108718600
Chr6L:3441997..3442016 17/20 Sense off-target LOC108719524
Chr9_10S:57369077..57369096 17/20 Sense off-target LOC108702260
Chr8L:62802605..62802624 16/20 Antisense off-target ckm.L
Chr5L:68870916..68870935 16/20 Antisense off-target LOC108716142
Chr8L:92544248..92544267 16/20 Sense off-target LOC108698815
Chr7S:309205..309224 19/20 Sense unknown
Chr5S:104763591..104763610 18/20 Antisense unknown
Chr6L:15207964..15207983 18/20 Sense unknown
Chr6L:82052360..82052379 18/20 Sense unknown
Chr1S:14529230..14529249 18/20 Antisense unknown
Chr5L:83592995..83593014 17/20 Antisense unknown
Chr5S:9821748..9821767 17/20 Sense unknown
Chr6S:136961340..136961359 17/20 Antisense unknown
Chr8L:113296173..113296192 17/20 Sense unknown
Chr9_10S:11713864..11713883 17/20 Antisense unknown
Chr1L:224255820..224255839 17/20 Antisense unknown
Chr1S:140101085..140101104 17/20 Antisense unknown
Chr5L:151899443..151899462 17/20 Sense unknown
Chr5L:23988066..23988085 17/20 Antisense unknown
Chr2L:183512388..183512407 17/20 Antisense unknown
Chr2L:183579821..183579840 17/20 Antisense unknown
Chr2L:89671976..89671995 17/20 Antisense unknown
Chr2S:122177381..122177400 17/20 Antisense unknown
Chr3S:123446561..123446580 17/20 Antisense unknown
Chr4S:102487907..102487926 17/20 Sense unknown
Chr4S:53953377..53953396 17/20 Sense unknown
Chr2L:133637909..133637928 16/20 Antisense unknown
Chr2S:63796685..63796704 16/20 Antisense unknown
tropicalis 10.0 Chr2:115907607..115907626 20/20 Sense target adprhl1
Chr3:127660096..127660115 19/20 Sense off-target nefl
Chr2:152587671..152587690 18/20 Sense off-target nbea
Chr3:127632632..127632651 18/20 Sense off-target nefm
Chr1:152714576..152714595 18/20 Sense off-target ggt5
Chr2:116351319..116351338 18/20 Sense off-target atp11a
Chr7:77783416..77783435 18/20 Sense off-target LOC100127873
Chr9:43860313..43860332 17/20 Antisense off-target stat4
Chr1:10977333..10977352 17/20 Sense off-target ctnna2
Chr7:125350254..125350273 17/20 Sense off-target LOC105945759
Chr1:58365848..58365867 19/20 Sense unknown
Chr6:91404243..91404262 18/20 Sense unknown
Chr7:63008393..63008412 18/20 Antisense unknown
Chr8:131281748..131281767 18/20 Sense unknown
Chr3:40867153..40867172 17/20 Sense unknown
Chr4:136240142..136240161 17/20 Sense unknown
Chr4:45557361..45557380 17/20 Sense unknown
Chr5:1128450..1128469 17/20 Sense unknown
Chr1:46865404..46865423 17/20 Sense unknown
Chr7:126721898..126721917 17/20 Sense unknown
Chr8:147091977..147091996 17/20 Sense unknown
Chr6:70005693..70005712 16/20 Antisense unknown
Publications
???morpholino.displaySummary.papersFirst??? Defective heart chamber growth and myofibrillogenesis after knockout of adprhl1 gene function by targeted disruption of the ancestral catalytic active site., PLoS One 2020     
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