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Summary Attributions Wiki
XB-GRNA-23659094
gRNA Name: adprhl1 gRNA8
Synonyms: -e2-1
Genomic Target: adprhl1

PAM Sequence: AGG
Sequence: 5' GGCACACCCCATTCAATGAAAA 3'



Genomic Target tropicalis laevis.L laevis.S
Identities 22/22 22/22 22/22
genome
gRNA
 
5' TTTTCATTGAATGGGGTGTGCC 3'
   ||||||||||||||||||||||
3' AAAAGTAACTTACCCCACACGG 5'
 
5' TTTTCATTGAATGGGGTGTGCC 3'
   ||||||||||||||||||||||
3' AAAAGTAACTTACCCCACACGG 5'
 
5' TTTTCATTGAATGGGGTGTGCC 3'
   ||||||||||||||||||||||
3' AAAAGTAACTTACCCCACACGG 5'
    
Genomic Alignments Position Identity Strand Target Genomic Target
laevis 10.1 Chr2L:120472610..120472631 22/22 Sense target adprhl1.L
Chr2S:106627452..106627473 22/22 Sense target adprhl1.S
Chr1L:109957717..109957738 18/22 Antisense off-target ptbp1.L
Chr1S:130441642..130441663 18/22 Sense off-target slc7a7.S
Chr4S:109377137..109377158 20/22 Antisense unknown
Chr2L:178790707..178790728 19/22 Sense unknown
Chr9_10S:87553531..87553552 19/22 Sense unknown
Chr4S:60194335..60194356 19/22 Sense unknown
Chr6S:117606130..117606151 18/22 Antisense unknown
Chr6S:21574479..21574500 18/22 Sense unknown
Chr6L:91667744..91667765 18/22 Antisense unknown
Chr1L:231336389..231336410 18/22 Antisense unknown
tropicalis 10.0 Chr2:115902715..115902736 22/22 Sense target adprhl1
Chr1:20874682..20874703 18/22 Sense off-target fgfr3
Chr3:57852345..57852366 18/22 Sense off-target tbc1d30
Chr6:87012610..87012631 18/22 Antisense unknown
Chr9:71437200..71437221 18/22 Sense unknown
Publications
???morpholino.displaySummary.papersFirst??? Defective heart chamber growth and myofibrillogenesis after knockout of adprhl1 gene function by targeted disruption of the ancestral catalytic active site., PLoS One 2020     
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