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Summary Attributions Wiki
XB-GRNA-23659095
gRNA Name: adprhl1 gRNA9
Synonyms: -e7-1S
Genomic Target: adprhl1

PAM Sequence: TGG
Sequence: 5' GCAGGAGAAGGTGGTGCCAC 3'



Genomic Target tropicalis laevis.L laevis.S
Identities 20/20 18/20 20/20
genome
gRNA
 
5' GTGGCACCACCTTCTCCTGC 3'
   ||||||||||||||||||||
3' CACCGTGGTGGAAGAGGACG 5'
 
5' GTAGCACCACCTTCTCCTGA 3'
   || |||||||||||||||| 
3' CACCGTGGTGGAAGAGGACG 5'
 
5' GTGGCACCACCTTCTCCTGC 3'
   ||||||||||||||||||||
3' CACCGTGGTGGAAGAGGACG 5'
    
Genomic Alignments Position Identity Strand Target Genomic Target
laevis 10.1 Chr2S:106633795..106633814 20/20 Sense target adprhl1.S
Chr2L:120477591..120477610 18/20 Sense target adprhl1.L
Chr6L:52909862..52909881 18/20 Antisense off-target ano10.L
Chr3L:144546902..144546921 18/20 Antisense off-target LOC108710362
Chr4L:153176252..153176271 16/20 Sense off-target LOC121403137
Chr4L:53225872..53225891 16/20 Sense off-target LOC121402811
Chr2S:41439204..41439223 18/20 Sense unknown
Chr7S:9964825..9964844 18/20 Antisense unknown
Chr1S:85229113..85229132 18/20 Sense unknown
Chr9_10L:20097171..20097190 17/20 Sense unknown
tropicalis 10.0 Chr2:115908672..115908691 20/20 Sense target adprhl1
Chr1:60148150..60148169 18/20 Sense off-target il15
Chr3:33131291..33131310 18/20 Antisense off-target slit3
Chr7:37064660..37064679 18/20 Antisense off-target hectd2
Chr1:43711244..43711263 18/20 Antisense off-target mtnr1a
Chr6:57137940..57137959 17/20 Antisense off-target stk17a
Chr8:141492514..141492533 17/20 Sense off-target slc25a44
Chr3:9299611..9299630 16/20 Antisense off-target adipor2
Chr1:56450796..56450815 16/20 Antisense off-target LOC116408478
Chr7:11982306..11982325 19/20 Sense unknown
Chr4:125119251..125119270 18/20 Sense unknown
Chr5:139197476..139197495 18/20 Sense unknown
Chr1:56467267..56467286 16/20 Sense unknown
Chr1:56198954..56198973 16/20 Sense unknown
Chr1:56221827..56221846 16/20 Sense unknown
Chr1:56336046..56336065 16/20 Sense unknown
Chr1:56386134..56386153 16/20 Sense unknown
Publications
???morpholino.displaySummary.papersFirst??? Defective heart chamber growth and myofibrillogenesis after knockout of adprhl1 gene function by targeted disruption of the ancestral catalytic active site., PLoS One 2020     
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