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Summary Attributions Wiki
XB-GRNA-23659096
gRNA Name: adprhl1 gRNA10
Synonyms: -e7-1L
Genomic Target: adprhl1

PAM Sequence: TGG
Sequence: 5' GCAGGAGAAGGTGGTGCTAC 3'



Genomic Target tropicalis laevis.L laevis.S
Identities 19/20 19/20 19/20
genome
gRNA
 
5' GTGGCACCACCTTCTCCTGC 3'
   || |||||||||||||||||
3' CATCGTGGTGGAAGAGGACG 5'
 
5' GTAGCACCACCTTCTCCTGA 3'
   ||||||||||||||||||| 
3' CATCGTGGTGGAAGAGGACG 5'
 
5' GTGGCACCACCTTCTCCTGC 3'
   || |||||||||||||||||
3' CATCGTGGTGGAAGAGGACG 5'
    
Genomic Alignments Position Identity Strand Target Genomic Target
laevis 10.1 Chr2L:120477591..120477610 19/20 Sense target adprhl1.L
Chr2S:106633795..106633814 19/20 Sense target adprhl1.S
Chr6L:52909862..52909881 17/20 Antisense off-target ano10.L
Chr4L:153176252..153176271 17/20 Sense off-target LOC121403137
Chr8S:103925877..103925896 18/20 Antisense unknown
Chr9_10L:109812874..109812893 18/20 Sense unknown
Chr4S:39583341..39583360 17/20 Sense unknown
Chr7S:9964825..9964844 17/20 Antisense unknown
Chr1L:2715029..2715048 17/20 Antisense unknown
Chr1S:117711056..117711075 16/20 Antisense unknown
tropicalis 10.0 Chr2:115908672..115908691 19/20 Sense target adprhl1
Chr1:85531719..85531738 19/20 Antisense off-target helq
Chr5:92507498..92507517 19/20 Antisense off-target asrgl1
Chr1:85531719..85531738 19/20 Antisense off-target LOC100485787
Chr5:27701009..27701028 18/20 Antisense off-target sptlc3
Chr6:18472247..18472266 17/20 Antisense off-target ncapg2
Chr7:37064660..37064679 17/20 Antisense off-target hectd2
Chr2:143730349..143730368 17/20 Sense off-target col2a1
Chr3:9299611..9299630 17/20 Antisense off-target adipor2
Chr6:34663585..34663604 19/20 Antisense unknown
Chr1:81422563..81422582 19/20 Sense unknown
Chr8:116015864..116015883 18/20 Antisense unknown
Chr6:55054270..55054289 18/20 Antisense unknown
Chr6:67037894..67037913 18/20 Antisense unknown
Chr7:11982306..11982325 18/20 Sense unknown
Chr3:141212700..141212719 16/20 Antisense unknown
Publications
???morpholino.displaySummary.papersFirst??? Defective heart chamber growth and myofibrillogenesis after knockout of adprhl1 gene function by targeted disruption of the ancestral catalytic active site., PLoS One 2020     
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