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Summary Attributions Wiki
XB-GRNA-23659114
gRNA Name: mdk gRNA1
Synonyms:
Genomic Target: mdk

PAM Sequence: GGG
Sequence: 5' AGACTGCGGAGCAGGAACTC 3'



Genomic Target tropicalis laevis.L laevis.S
Identities 20/20 19/20 19/20
genome
gRNA
 
5' GAGTTCCTGCTCCGCAGTCT 3'
   ||||||||||||||||||||
3' CTCAAGGACGAGGCGTCAGA 5'
 
5' GAGTTCCGGCTCCGCAGTCT 3'
   ||||||| ||||||||||||
3' CTCAAGGACGAGGCGTCAGA 5'
 
5' GAGTTCCAGCTCCGCAGTCT 3'
   ||||||| ||||||||||||
3' CTCAAGGACGAGGCGTCAGA 5'
    
Genomic Alignments Position Identity Strand Target Genomic Target
laevis 10.1 Chr4L:9591459..9591478 19/20 Antisense target mdk.L
Chr4S:14528158..14528177 19/20 Sense target mdk.S
Chr6S:28217792..28217811 17/20 Antisense off-target thsd7a.S
Chr4S:25292658..25292677 16/20 Sense unknown
tropicalis 10.0 Chr4:13271655..13271674 20/20 Antisense target mdk
Chr5:112474663..112474682 17/20 Sense off-target mecom
Publications
???morpholino.displaySummary.papersFirst??? Maximizing CRISPR/Cas9 phenotype penetrance applying predictive modeling of editing outcomes in Xenopus and zebrafish embryos., Sci Rep 2020     
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