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Summary Attributions Wiki
XB-GRNA-23659121
gRNA Name: tpcn1 gRNA2
Synonyms: tpcn_g2
Genomic Target: tpcn1

PAM Sequence: AGG
Sequence: 5' GGGGATCACATTAGAGATGGAGG 3'



Genomic Target tropicalis laevis.L
Identities 22/23 22/23
genome
gRNA
 
5' CCTCCATCTCTAAGGTGATCCCC 3'
   ||||||||||||| |||||||||
3' GGAGGTAGAGATTACACTAGGGG 5'
 
5' CCTCCATCTCTAAGGTGATCCCC 3'
   ||||||||||||| |||||||||
3' GGAGGTAGAGATTACACTAGGGG 5'
     
Genomic Alignments Position Identity Strand Target Genomic Target
laevis 10.1 Chr1L:157177463..157177485 22/23 Sense target tpcn1.L
Chr6L:42233116..42233138 20/23 Sense off-target osbpl3.L
Chr9_10S:94188700..94188722 19/23 Sense off-target adap1.S
Chr6L:157828103..157828125 19/23 Sense off-target trappc9.L
Chr4L:120338064..120338086 19/23 Sense off-target baiap2l2.L
Chr8S:80754488..80754510 19/23 Sense unknown
Chr9_10L:53307722..53307744 19/23 Antisense unknown
tropicalis 10.0 Chr1:145311773..145311795 22/23 Sense target tpcn1
Chr8:105216070..105216092 19/23 Antisense off-target tyro3
Chr4:121428117..121428139 19/23 Antisense off-target cacna1i
Publications
???morpholino.displaySummary.papersFirst??? Maximizing CRISPR/Cas9 phenotype penetrance applying predictive modeling of editing outcomes in Xenopus and zebrafish embryos., Sci Rep 2020     
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