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Summary Attributions Wiki
XB-GRNA-23659127
gRNA Name: fbxw7 gRNA1
Synonyms:
Genomic Target: fbxw7

PAM Sequence: GGG
Sequence: 5' AGTGTCTGAGAACACTTGTT 3'



Genomic Target tropicalis laevis.L
Identities 20/20 18/20
genome
gRNA
 
5' AACAAGTGTTCTCAGACACT 3'
   ||||||||||||||||||||
3' TTGTTCACAAGAGTCTGTGA 5'
 
5' GACAAGTGTTCTTAGACACT 3'
    ||||||||||| |||||||
3' TTGTTCACAAGAGTCTGTGA 5'
     
Genomic Alignments Position Identity Strand Target Genomic Target
laevis 9.2 chr1L:48664207..48664226 18/20 Sense target fbxw7.L
chr1S:44336950..44336969 19/20 Sense off-target LOC108706660 [provisional:fbxw7]
chr4L:124577578..124577597 18/20 Sense off-target grm7.L
chr6S:68553191..68553210 17/20 Antisense off-target drosha.S
chr9_10S:68805106..68805125 17/20 Antisense off-target LOC108703039 [provisional:erbb4]
chr9_10S:1806441..1806460 16/20 Sense off-target ikzf3.S
chr4S:53392903..53392922 16/20 Antisense off-target adgrl2.S
chr9_10S:1806441..1806460 16/20 Sense off-target LOC108702488
chr6L:46683717..46683736 18/20 Antisense unknown
chr1S:66022662..66022681 16/20 Sense unknown
chr2L:58407785..58407804 16/20 Antisense unknown
chr6L:88452770..88452789 16/20 Antisense unknown
tropicalis 10.0 Chr1:55548634..55548653 20/20 Sense target fbxw7
Chr2:149456711..149456730 18/20 Sense off-target LOC108645818
Chr4:90418780..90418799 17/20 Antisense off-target LOC100492579
Chr7:51557877..51557896 17/20 Sense unknown
Chr1:121957977..121957996 16/20 Sense unknown
Publications
???morpholino.displaySummary.papersFirst??? Maximizing CRISPR/Cas9 phenotype penetrance applying predictive modeling of editing outcomes in Xenopus and zebrafish embryos., Sci Rep 2020     
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